MicroRNA-200, associated with metastatic breast cancer, promotes traits of mammary luminal progenitor cells

Sánchez-Cid Pérez, Lourdes; Pons, Mònica; Lozano Salvatella, Juan José; Rubio, Nuria; Guerra-Rebollo, Marta; Soriano, Aroa; Paris-Coderch, Laia; Segura, Miguel F.; Fueyo, Raquel; Arguimbau, Judit; Zodda, Erika; Bermudo, Raquel; Alonso Vargas, María Inmaculada; Caparrós, Xavier; Cascante i Serratosa, Marta; Rafii, Arash; Kang, Yibin; Martínez Balbás, Marian; Weiss, Stephen J.; Blanco Fernández, Jerónimo; Muñoz, Montserrat; Fernández Ruiz, Pedro Luis; Thomsom, Timothy M.

MicroRNA-200, associated with metastatic breast cancer, promotes traits of mammary luminal progenitor cells

MicroRNAs are critical regulators of gene networks in normal and abnormal biological processes. Focusing on invasive ductal breast cancer (IDC), we have found dysregulated expression in tumor samples of several microRNAs, including the miR-200 family, along progression from primary tumors to distant...

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Autores:	Sánchez-Cid Pérez, Lourdes, Pons, Mònica, Lozano Salvatella, Juan José, Rubio, Nuria, Guerra-Rebollo, Marta, Soriano, Aroa, Paris-Coderch, Laia, Segura, Miguel F., Fueyo, Raquel, Arguimbau, Judit, Zodda, Erika, Bermudo, Raquel, Alonso Vargas, María Inmaculada, Caparrós, Xavier, Cascante i Serratosa, Marta, Rafii, Arash, Kang, Yibin, Martínez Balbás, Marian, Weiss, Stephen J., Blanco Fernández, Jerónimo, Muñoz, Montserrat, Fernández Ruiz, Pedro Luis, Thomsom, Timothy M.
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2017
País:	España
Institución:	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:	Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:	oai:recercat.cat:2445/127217
Acceso en línea:	https://hdl.handle.net/2445/127217
Access Level:	acceso abierto
Palabra clave:	Micro RNAs Càncer de mama Cèl·lules epitelials MicroRNAs Breast cancer Epithelial cells

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spelling	MicroRNA-200, associated with metastatic breast cancer, promotes traits of mammary luminal progenitor cellsSánchez-Cid Pérez, LourdesPons, MònicaLozano Salvatella, Juan JoséRubio, NuriaGuerra-Rebollo, MartaSoriano, AroaParis-Coderch, LaiaSegura, Miguel F.Fueyo, RaquelArguimbau, JuditZodda, ErikaBermudo, RaquelAlonso Vargas, María InmaculadaCaparrós, XavierCascante i Serratosa, MartaRafii, ArashKang, YibinMartínez Balbás, MarianWeiss, Stephen J.Blanco Fernández, JerónimoMuñoz, MontserratFernández Ruiz, Pedro LuisThomsom, Timothy M.Micro RNAsCàncer de mamaCèl·lules epitelialsMicroRNAsBreast cancerEpithelial cellsMicroRNAs are critical regulators of gene networks in normal and abnormal biological processes. Focusing on invasive ductal breast cancer (IDC), we have found dysregulated expression in tumor samples of several microRNAs, including the miR-200 family, along progression from primary tumors to distant metastases, further reflected in higher blood levels of miR-200b and miR-7 in IDC patients with regional or distant metastases relative to patients with primary node-negative tumors. Forced expression of miR-200s in MCF10CA1h mammary cells induced an enhanced epithelial program, aldehyde dehydrogenase (ALDH) activity, mammosphere growth and ability to form branched tubuloalveolar structures while promoting orthotopic tumor growth and lung colonization in vivo. MiR-200s also induced the constitutive activation of the PI3K-Akt signaling through downregulation of PTEN, and the enhanced mammosphere growth and ALDH activity induced in MCF10CA1h cells by miR-200s required the activation of this signaling pathway. Interestingly, the morphology of tumors formed in vivo by cells expressing miR-200s was reminiscent of metaplastic breast cancer (MBC). Indeed, the epithelial components of MBC samples expressed significantly higher levels of miR-200s than their mesenchymal components and displayed a marker profile compatible with luminal progenitor cells. We propose that microRNAs of the miR-200 family promote traits of highly proliferative breast luminal progenitor cells, thereby exacerbating the growth and metastatic properties of transformed mammary epithelial cells.Impact Journals2019201920172019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion23 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/127217Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.18632/oncotarget.20698Oncotarget, 2017, vol. 8, num. 48https://doi.org/10.18632/oncotarget.20698cc-by (c) Sánchez-Cid Pérez, Lourdes et al., 2017http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1272172026-05-29T05:05:01Z
dc.title.none.fl_str_mv	MicroRNA-200, associated with metastatic breast cancer, promotes traits of mammary luminal progenitor cells
title	MicroRNA-200, associated with metastatic breast cancer, promotes traits of mammary luminal progenitor cells
spellingShingle	MicroRNA-200, associated with metastatic breast cancer, promotes traits of mammary luminal progenitor cells Sánchez-Cid Pérez, Lourdes Micro RNAs Càncer de mama Cèl·lules epitelials MicroRNAs Breast cancer Epithelial cells
title_short	MicroRNA-200, associated with metastatic breast cancer, promotes traits of mammary luminal progenitor cells
title_full	MicroRNA-200, associated with metastatic breast cancer, promotes traits of mammary luminal progenitor cells
title_fullStr	MicroRNA-200, associated with metastatic breast cancer, promotes traits of mammary luminal progenitor cells
title_full_unstemmed	MicroRNA-200, associated with metastatic breast cancer, promotes traits of mammary luminal progenitor cells
title_sort	MicroRNA-200, associated with metastatic breast cancer, promotes traits of mammary luminal progenitor cells
dc.creator.none.fl_str_mv	Sánchez-Cid Pérez, Lourdes Pons, Mònica Lozano Salvatella, Juan José Rubio, Nuria Guerra-Rebollo, Marta Soriano, Aroa Paris-Coderch, Laia Segura, Miguel F. Fueyo, Raquel Arguimbau, Judit Zodda, Erika Bermudo, Raquel Alonso Vargas, María Inmaculada Caparrós, Xavier Cascante i Serratosa, Marta Rafii, Arash Kang, Yibin Martínez Balbás, Marian Weiss, Stephen J. Blanco Fernández, Jerónimo Muñoz, Montserrat Fernández Ruiz, Pedro Luis Thomsom, Timothy M.
author	Sánchez-Cid Pérez, Lourdes
author_facet	Sánchez-Cid Pérez, Lourdes Pons, Mònica Lozano Salvatella, Juan José Rubio, Nuria Guerra-Rebollo, Marta Soriano, Aroa Paris-Coderch, Laia Segura, Miguel F. Fueyo, Raquel Arguimbau, Judit Zodda, Erika Bermudo, Raquel Alonso Vargas, María Inmaculada Caparrós, Xavier Cascante i Serratosa, Marta Rafii, Arash Kang, Yibin Martínez Balbás, Marian Weiss, Stephen J. Blanco Fernández, Jerónimo Muñoz, Montserrat Fernández Ruiz, Pedro Luis Thomsom, Timothy M.
author_role	author
author2	Pons, Mònica Lozano Salvatella, Juan José Rubio, Nuria Guerra-Rebollo, Marta Soriano, Aroa Paris-Coderch, Laia Segura, Miguel F. Fueyo, Raquel Arguimbau, Judit Zodda, Erika Bermudo, Raquel Alonso Vargas, María Inmaculada Caparrós, Xavier Cascante i Serratosa, Marta Rafii, Arash Kang, Yibin Martínez Balbás, Marian Weiss, Stephen J. Blanco Fernández, Jerónimo Muñoz, Montserrat Fernández Ruiz, Pedro Luis Thomsom, Timothy M.
author2_role	author author author author author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Micro RNAs Càncer de mama Cèl·lules epitelials MicroRNAs Breast cancer Epithelial cells
topic	Micro RNAs Càncer de mama Cèl·lules epitelials MicroRNAs Breast cancer Epithelial cells
description	MicroRNAs are critical regulators of gene networks in normal and abnormal biological processes. Focusing on invasive ductal breast cancer (IDC), we have found dysregulated expression in tumor samples of several microRNAs, including the miR-200 family, along progression from primary tumors to distant metastases, further reflected in higher blood levels of miR-200b and miR-7 in IDC patients with regional or distant metastases relative to patients with primary node-negative tumors. Forced expression of miR-200s in MCF10CA1h mammary cells induced an enhanced epithelial program, aldehyde dehydrogenase (ALDH) activity, mammosphere growth and ability to form branched tubuloalveolar structures while promoting orthotopic tumor growth and lung colonization in vivo. MiR-200s also induced the constitutive activation of the PI3K-Akt signaling through downregulation of PTEN, and the enhanced mammosphere growth and ALDH activity induced in MCF10CA1h cells by miR-200s required the activation of this signaling pathway. Interestingly, the morphology of tumors formed in vivo by cells expressing miR-200s was reminiscent of metaplastic breast cancer (MBC). Indeed, the epithelial components of MBC samples expressed significantly higher levels of miR-200s than their mesenchymal components and displayed a marker profile compatible with luminal progenitor cells. We propose that microRNAs of the miR-200 family promote traits of highly proliferative breast luminal progenitor cells, thereby exacerbating the growth and metastatic properties of transformed mammary epithelial cells.
publishDate	2017
dc.date.none.fl_str_mv	2017 2019 2019 2019
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://hdl.handle.net/2445/127217
url	https://hdl.handle.net/2445/127217
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Reproducció del document publicat a: https://doi.org/10.18632/oncotarget.20698 Oncotarget, 2017, vol. 8, num. 48 https://doi.org/10.18632/oncotarget.20698
dc.rights.none.fl_str_mv	cc-by (c) Sánchez-Cid Pérez, Lourdes et al., 2017 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess
rights_invalid_str_mv	cc-by (c) Sánchez-Cid Pérez, Lourdes et al., 2017 http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	23 p. application/pdf application/pdf
dc.publisher.none.fl_str_mv	Impact Journals
publisher.none.fl_str_mv	Impact Journals
dc.source.none.fl_str_mv	Articles publicats en revistes (Bioquímica i Biomedicina Molecular) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str	Recercat. Dipósit de la Recerca de Catalunya
collection	Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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MicroRNA-200, associated with metastatic breast cancer, promotes traits of mammary luminal progenitor cells

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