Thermocaproicibacter melissae gen. nov., sp. nov., a thermophilic chain-elongating bacterium, producing n-caproate from polymeric carbohydrates

Strain MDTJ8T is a chain-elongating thermophilic bacterium isolated from a thermophilic acidogenic anaerobic digestor treating human waste while producing the high commodity chemical n-caproate. The strain grows and produces formate, acetate, n-butyrate, n-caproate and lactate from mono-, di- and po...

Descripción completa

Detalles Bibliográficos
Autores: Nguyen, Tinh van, Viver, Tomeu, Smets, Ilse, Bernaerts, Kristel, Faust, Karoline, Lavigne, Rob, Poughon, Laurent, Dussap, Claude-Gilles, Springael, Dirk
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/338729
Acceso en línea:http://hdl.handle.net/10261/338729
https://api.elsevier.com/content/abstract/scopus_id/85159715515
Access Level:acceso abierto
Palabra clave:Oscillospiraceae
Thermocaproicibacter melissae
Anaerobic digestion
n-caproate
Novel genus
Thermophilic chain elongating bacteria
id ES_71fc9170c6423b1ce4e8bc85a1fd6d69
oai_identifier_str oai:digital.csic.es:10261/338729
network_acronym_str ES
network_name_str España
repository_id_str
spelling Thermocaproicibacter melissae gen. nov., sp. nov., a thermophilic chain-elongating bacterium, producing n-caproate from polymeric carbohydratesNguyen, Tinh vanViver, TomeuSmets, IlseBernaerts, KristelFaust, KarolineLavigne, RobPoughon, LaurentDussap, Claude-GillesSpringael, DirkOscillospiraceaeThermocaproicibacter melissaeAnaerobic digestionn-caproateNovel genusThermophilic chain elongating bacteriaStrain MDTJ8T is a chain-elongating thermophilic bacterium isolated from a thermophilic acidogenic anaerobic digestor treating human waste while producing the high commodity chemical n-caproate. The strain grows and produces formate, acetate, n-butyrate, n-caproate and lactate from mono-, di- and polymeric saccharides at 37-60 °C (optimum, 50-55 °C) and at pH 5.0-7.0 (optimum, pH 6.5). The organism is an obligate anaerobe, is motile and its cells form rods (0.3-0.5×1.0-3.0 µm) that stain Gram-positive and occur primarily as chains. Phylogenetic analysis of both the 16S rRNA gene and full genome sequence shows that strain MDTJ8T belongs to a group that consists of mesophylic chain-elongating bacteria within the family Oscillospiraceae, being nearest to Caproicibacter fermentans EA1T (94.8 %) and Caproiciproducens galactitolivorans BS-1T (93.7 %). Its genome (1.96 Mbp) with a G+C content of 49.6 mol% is remarkably smaller than those of other chain-elongating bacteria of the family Oscillospiraceae. Pairwise average nucleotide identity and DNA-DNA hybridization values between strain MDJT8T and its mesophilic family members are less than 70 and 35 %, respectively, while pairwise average amino acid identity values are less than 68 %. In addition, strain MDJT8T uses far less carbohydrate and non-carbohydrate substrates compared to its nearest family members. The predominant cellular fatty acids of strain MDTJ8T are C14 : 0, C14 : 0 DMA (dimethyl acetal) and C16 : 0, while its polar lipid profile shows three unidentified glycophospholipids, 11 glycolipids, 13 phospholipids and six unidentified lipids. No respiratory quinones and polyamines are detected. Based on its phylogenetic, genotypic, morphological, physiological, biochemical and chemotaxonomic characteristics, strain MDTJ8T represents a novel species and novel genus of the family Oscillospiraceae and Thermocaproicibacter melissae gen. nov., sp. nov. is proposed as its name. The type strain is MDTJ8T (=DSM 114174T=LMG 32615T=NCCB 100883T).This project was supported by the MELiSSA PhD POMP programme (Grant no: 4000114262/15 /NL/JC).Peer reviewedMicrobiology SocietyMelissa FoundationConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/338729https://api.elsevier.com/content/abstract/scopus_id/85159715515reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésNguyen, Tinh van; Viver, Tomeu; Smets, Ilse; Bernaerts, Kristel; Faust, Karoline; Lavigne, Rob; Poughon, Laurent; Dussap, Claude-Gilles; Springael, Dirk; 2023; Supplementary material Thermocaproicibacter melissae gen. nov., sp. nov., a thermophilic chain elongating bacterium, producing n-caproate from polymeric carbohydrates [Dataset]; Microbiology Society; https://doi.org/10.1099/ijsem.0.005893https://doi.org/10.1099/ijsem.0.005893Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3387292026-05-22T06:33:51Z
dc.title.none.fl_str_mv Thermocaproicibacter melissae gen. nov., sp. nov., a thermophilic chain-elongating bacterium, producing n-caproate from polymeric carbohydrates
title Thermocaproicibacter melissae gen. nov., sp. nov., a thermophilic chain-elongating bacterium, producing n-caproate from polymeric carbohydrates
spellingShingle Thermocaproicibacter melissae gen. nov., sp. nov., a thermophilic chain-elongating bacterium, producing n-caproate from polymeric carbohydrates
Nguyen, Tinh van
Oscillospiraceae
Thermocaproicibacter melissae
Anaerobic digestion
n-caproate
Novel genus
Thermophilic chain elongating bacteria
title_short Thermocaproicibacter melissae gen. nov., sp. nov., a thermophilic chain-elongating bacterium, producing n-caproate from polymeric carbohydrates
title_full Thermocaproicibacter melissae gen. nov., sp. nov., a thermophilic chain-elongating bacterium, producing n-caproate from polymeric carbohydrates
title_fullStr Thermocaproicibacter melissae gen. nov., sp. nov., a thermophilic chain-elongating bacterium, producing n-caproate from polymeric carbohydrates
title_full_unstemmed Thermocaproicibacter melissae gen. nov., sp. nov., a thermophilic chain-elongating bacterium, producing n-caproate from polymeric carbohydrates
title_sort Thermocaproicibacter melissae gen. nov., sp. nov., a thermophilic chain-elongating bacterium, producing n-caproate from polymeric carbohydrates
dc.creator.none.fl_str_mv Nguyen, Tinh van
Viver, Tomeu
Smets, Ilse
Bernaerts, Kristel
Faust, Karoline
Lavigne, Rob
Poughon, Laurent
Dussap, Claude-Gilles
Springael, Dirk
author Nguyen, Tinh van
author_facet Nguyen, Tinh van
Viver, Tomeu
Smets, Ilse
Bernaerts, Kristel
Faust, Karoline
Lavigne, Rob
Poughon, Laurent
Dussap, Claude-Gilles
Springael, Dirk
author_role author
author2 Viver, Tomeu
Smets, Ilse
Bernaerts, Kristel
Faust, Karoline
Lavigne, Rob
Poughon, Laurent
Dussap, Claude-Gilles
Springael, Dirk
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Melissa Foundation
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Oscillospiraceae
Thermocaproicibacter melissae
Anaerobic digestion
n-caproate
Novel genus
Thermophilic chain elongating bacteria
topic Oscillospiraceae
Thermocaproicibacter melissae
Anaerobic digestion
n-caproate
Novel genus
Thermophilic chain elongating bacteria
description Strain MDTJ8T is a chain-elongating thermophilic bacterium isolated from a thermophilic acidogenic anaerobic digestor treating human waste while producing the high commodity chemical n-caproate. The strain grows and produces formate, acetate, n-butyrate, n-caproate and lactate from mono-, di- and polymeric saccharides at 37-60 °C (optimum, 50-55 °C) and at pH 5.0-7.0 (optimum, pH 6.5). The organism is an obligate anaerobe, is motile and its cells form rods (0.3-0.5×1.0-3.0 µm) that stain Gram-positive and occur primarily as chains. Phylogenetic analysis of both the 16S rRNA gene and full genome sequence shows that strain MDTJ8T belongs to a group that consists of mesophylic chain-elongating bacteria within the family Oscillospiraceae, being nearest to Caproicibacter fermentans EA1T (94.8 %) and Caproiciproducens galactitolivorans BS-1T (93.7 %). Its genome (1.96 Mbp) with a G+C content of 49.6 mol% is remarkably smaller than those of other chain-elongating bacteria of the family Oscillospiraceae. Pairwise average nucleotide identity and DNA-DNA hybridization values between strain MDJT8T and its mesophilic family members are less than 70 and 35 %, respectively, while pairwise average amino acid identity values are less than 68 %. In addition, strain MDJT8T uses far less carbohydrate and non-carbohydrate substrates compared to its nearest family members. The predominant cellular fatty acids of strain MDTJ8T are C14 : 0, C14 : 0 DMA (dimethyl acetal) and C16 : 0, while its polar lipid profile shows three unidentified glycophospholipids, 11 glycolipids, 13 phospholipids and six unidentified lipids. No respiratory quinones and polyamines are detected. Based on its phylogenetic, genotypic, morphological, physiological, biochemical and chemotaxonomic characteristics, strain MDTJ8T represents a novel species and novel genus of the family Oscillospiraceae and Thermocaproicibacter melissae gen. nov., sp. nov. is proposed as its name. The type strain is MDTJ8T (=DSM 114174T=LMG 32615T=NCCB 100883T).
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/338729
https://api.elsevier.com/content/abstract/scopus_id/85159715515
url http://hdl.handle.net/10261/338729
https://api.elsevier.com/content/abstract/scopus_id/85159715515
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Nguyen, Tinh van; Viver, Tomeu; Smets, Ilse; Bernaerts, Kristel; Faust, Karoline; Lavigne, Rob; Poughon, Laurent; Dussap, Claude-Gilles; Springael, Dirk; 2023; Supplementary material Thermocaproicibacter melissae gen. nov., sp. nov., a thermophilic chain elongating bacterium, producing n-caproate from polymeric carbohydrates [Dataset]; Microbiology Society; https://doi.org/10.1099/ijsem.0.005893
https://doi.org/10.1099/ijsem.0.005893
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410691867213824
score 15,812455

Similares en LA Referencia