Overexpression of SepJ alters septal morphology and heterocyst pattern regulated by diffusible signals in Anabaena

Filamentous, N2 -fixing, heterocyst-forming cyanobacteria grow as chains of cells that are connected by septal junctions. In the model organism Anabaena sp. strain PCC 7120, the septal protein SepJ is required for filament integrity, normal intercellular molecular exchange, heterocyst differentiatio...

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Autores: Mariscal Romero, Vicente, Herrero Moreno, Antonia, Flores García, Enrique
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2016
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/68543
Acceso en línea:http://hdl.handle.net/11441/68543
https://doi.org/10.1111/mmi.13436.
Access Level:acceso abierto
Palabra clave:Bacterial development
Cyanobacteria
Heterocyst differentiation
Intercellular communication
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spelling Overexpression of SepJ alters septal morphology and heterocyst pattern regulated by diffusible signals in AnabaenaMariscal Romero, VicenteHerrero Moreno, AntoniaFlores García, EnriqueBacterial developmentCyanobacteriaHeterocyst differentiationIntercellular communicationFilamentous, N2 -fixing, heterocyst-forming cyanobacteria grow as chains of cells that are connected by septal junctions. In the model organism Anabaena sp. strain PCC 7120, the septal protein SepJ is required for filament integrity, normal intercellular molecular exchange, heterocyst differentiation, and diazotrophic growth. An Anabaena strain overexpressing SepJ made wider septa between vegetative cells than the wild type, which correlated with a more spread location of SepJ in the septa as observed with a SepJ-GFP fusion, and contained an increased number of nanopores, the septal peptidoglycan perforations that likely accommodate septal junctions. The septa between heterocysts and vegetative cells, which are narrow in wild-type Anabaena, were notably enlarged in the SepJ-overexpressing mutant. Intercellular molecular exchange tested with fluorescent tracers was increased for the SepJ-overexpressing strain specifically in the case of calcein transfer between vegetative cells and heterocysts. These results support an association between calcein transfer, SepJ-related septal junctions, and septal peptidoglycan nanopores. Under nitrogen deprivation, the SepJ-overexpressing strain produced an increased number of contiguous heterocysts but a decreased percentage of total heterocysts. These effects were lost or altered in patS and hetN mutant backgrounds, supporting a role of SepJ in the intercellular transfer of regulatory signals for heterocyst differentiationEspaña, Plan nacional de Investigación BFU2014-56757-PWileyBioquímica Vegetal y Biología Molecular2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/68543https://doi.org/10.1111/mmi.13436.reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMolecular Microbiology, 101 (6), 968-981.BFU2014-56757-Phttp://onlinelibrary.wiley.com/doi/10.1111/mmi.13436/epdfinfo:eu-repo/semantics/openAccessoai:idus.us.es:11441/685432026-06-17T12:51:07Z
dc.title.none.fl_str_mv Overexpression of SepJ alters septal morphology and heterocyst pattern regulated by diffusible signals in Anabaena
title Overexpression of SepJ alters septal morphology and heterocyst pattern regulated by diffusible signals in Anabaena
spellingShingle Overexpression of SepJ alters septal morphology and heterocyst pattern regulated by diffusible signals in Anabaena
Mariscal Romero, Vicente
Bacterial development
Cyanobacteria
Heterocyst differentiation
Intercellular communication
title_short Overexpression of SepJ alters septal morphology and heterocyst pattern regulated by diffusible signals in Anabaena
title_full Overexpression of SepJ alters septal morphology and heterocyst pattern regulated by diffusible signals in Anabaena
title_fullStr Overexpression of SepJ alters septal morphology and heterocyst pattern regulated by diffusible signals in Anabaena
title_full_unstemmed Overexpression of SepJ alters septal morphology and heterocyst pattern regulated by diffusible signals in Anabaena
title_sort Overexpression of SepJ alters septal morphology and heterocyst pattern regulated by diffusible signals in Anabaena
dc.creator.none.fl_str_mv Mariscal Romero, Vicente
Herrero Moreno, Antonia
Flores García, Enrique
author Mariscal Romero, Vicente
author_facet Mariscal Romero, Vicente
Herrero Moreno, Antonia
Flores García, Enrique
author_role author
author2 Herrero Moreno, Antonia
Flores García, Enrique
author2_role author
author
dc.contributor.none.fl_str_mv Bioquímica Vegetal y Biología Molecular
dc.subject.none.fl_str_mv Bacterial development
Cyanobacteria
Heterocyst differentiation
Intercellular communication
topic Bacterial development
Cyanobacteria
Heterocyst differentiation
Intercellular communication
description Filamentous, N2 -fixing, heterocyst-forming cyanobacteria grow as chains of cells that are connected by septal junctions. In the model organism Anabaena sp. strain PCC 7120, the septal protein SepJ is required for filament integrity, normal intercellular molecular exchange, heterocyst differentiation, and diazotrophic growth. An Anabaena strain overexpressing SepJ made wider septa between vegetative cells than the wild type, which correlated with a more spread location of SepJ in the septa as observed with a SepJ-GFP fusion, and contained an increased number of nanopores, the septal peptidoglycan perforations that likely accommodate septal junctions. The septa between heterocysts and vegetative cells, which are narrow in wild-type Anabaena, were notably enlarged in the SepJ-overexpressing mutant. Intercellular molecular exchange tested with fluorescent tracers was increased for the SepJ-overexpressing strain specifically in the case of calcein transfer between vegetative cells and heterocysts. These results support an association between calcein transfer, SepJ-related septal junctions, and septal peptidoglycan nanopores. Under nitrogen deprivation, the SepJ-overexpressing strain produced an increased number of contiguous heterocysts but a decreased percentage of total heterocysts. These effects were lost or altered in patS and hetN mutant backgrounds, supporting a role of SepJ in the intercellular transfer of regulatory signals for heterocyst differentiation
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/68543
https://doi.org/10.1111/mmi.13436.
url http://hdl.handle.net/11441/68543
https://doi.org/10.1111/mmi.13436.
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Molecular Microbiology, 101 (6), 968-981.
BFU2014-56757-P
http://onlinelibrary.wiley.com/doi/10.1111/mmi.13436/epdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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