Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA Transcripts

[EN] Sperm capacitation is a stepwise complex biochemical process towards fertilization. It in-cludes a crucial early calcium (Ca2+) transport mediated by CatSper channels and Canonical Transient Potential Channels (TRPC). We studied the relative abundance of mRNA transcripts changes of the CatSper...

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Autores: Lacalle Fernández, Estíbaliz, Consuegra González, César, Martínez Serrano, Cristina Alicia, Hidalgo Prieto, Manuel, Dorado Martín, Jesús M., Martínez Pastor, Felipe, Álvarez Rodríguez, Manuel, Rodríguez Martínez, Heriberto
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/21322
Acceso en línea:https://www.mdpi.com/2076-2615/12/8/1012
https://hdl.handle.net/10612/21322
Access Level:acceso abierto
Palabra clave:Biología
Veterinaria
Calcium channels
CatSper subunits
TRPC
Pig
Capacitation
2407 Biología Celular
3104.11 Reproducción
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repository_id_str
spelling Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA TranscriptsLacalle Fernández, EstíbalizConsuegra González, CésarMartínez Serrano, Cristina AliciaHidalgo Prieto, ManuelDorado Martín, Jesús M.Martínez Pastor, FelipeÁlvarez Rodríguez, ManuelRodríguez Martínez, HeribertoBiologíaVeterinariaCalcium channelsCatSper subunitsTRPCPigCapacitation2407 Biología Celular3104.11 Reproducción[EN] Sperm capacitation is a stepwise complex biochemical process towards fertilization. It in-cludes a crucial early calcium (Ca2+) transport mediated by CatSper channels and Canonical Transient Potential Channels (TRPC). We studied the relative abundance of mRNA transcripts changes of the CatSper β, γ and δ subunits and TRPC-channels 1, 3, 4, 6 and 7 in pig spermatozoa, after trigger-ing in vitro capacitation by bicarbonate ions at levels present in vivo at the fertilization site. For this purpose, we analyzedfive5 ejaculate pools (from three fertile adult boars) before (control-fresh sam-ples) and after in vitro exposure to capacitation conditions (37 mM NaHCO3, 2.25 mM CaCl2, 2 mM caffeine, 0.5% bovine serum albumin and 310 mM lactose) at 38 °C, 5% CO2 for 30 min. In vitro capacitation using bicarbonate elicits an increase in the relative abundance of mRNA transcripts of almost all studied Ca2+ channels, except CatSper-δ and TRPC1 (significantly reduced). These findings open new avenues of research to identify the specific role of each channel in boar sperm capacitation and elucidate the physiological meaning of the changes on sperm mRNA cargoSIThis research was funded by the Research Council FORMAS, Stockholm (Project 2017-00946 and Project 2019-00288) and by the Grant PID2019-108320RJ-I00 and IJCI-2015-24380 funded by MCIN/AEI/10.13039/501100011033 (Spain) and FEDER funds (EU). César Consuegra was supported by FPU Spanish fellowship (16/05745) from MECD (“Ministerio de Educación, Cultura y Deporte”), Madrid, Spain. Estíbaliz Lacalle was supported by the Spanish Ministry of Science, Innovation and Universities (MICINN Research “Retos” RTI2018-095183-B-I00), Madrid, SpainTo QualiSperm (AKYmed, Cheseaux-sur-Lausanne, Switzerland) for assisting with computerized sperm motility and kinetics assessment. Estíbaliz Lacalle was supported by a travel grant from University of León. César Consuegra was supported by FPU travel grant (FPU (EST19/00092) from MECD (“Ministerio de Educación, Cultura y Deporte”), Madrid, Spain.MDPIBiologia CelularFacultad de Ciencias Biologicas y Ambientales2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.mdpi.com/2076-2615/12/8/1012https://hdl.handle.net/10612/21322reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/Swedish Research Council FORMAS, Stockholm Sweden//Projects 2017-00946 and 2019-00288info:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/PID2019-108320RJ-I00info:eu-repo/grantAgreement/MINECO/Programa Estatal de Promoción del Talento y su Empleabilidad/IJCI-2015-24380info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018-095183-B-I00http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/213222026-06-24T12:43:27Z
dc.title.none.fl_str_mv Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA Transcripts
title Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA Transcripts
spellingShingle Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA Transcripts
Lacalle Fernández, Estíbaliz
Biología
Veterinaria
Calcium channels
CatSper subunits
TRPC
Pig
Capacitation
2407 Biología Celular
3104.11 Reproducción
title_short Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA Transcripts
title_full Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA Transcripts
title_fullStr Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA Transcripts
title_full_unstemmed Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA Transcripts
title_sort Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA Transcripts
dc.creator.none.fl_str_mv Lacalle Fernández, Estíbaliz
Consuegra González, César
Martínez Serrano, Cristina Alicia
Hidalgo Prieto, Manuel
Dorado Martín, Jesús M.
Martínez Pastor, Felipe
Álvarez Rodríguez, Manuel
Rodríguez Martínez, Heriberto
author Lacalle Fernández, Estíbaliz
author_facet Lacalle Fernández, Estíbaliz
Consuegra González, César
Martínez Serrano, Cristina Alicia
Hidalgo Prieto, Manuel
Dorado Martín, Jesús M.
Martínez Pastor, Felipe
Álvarez Rodríguez, Manuel
Rodríguez Martínez, Heriberto
author_role author
author2 Consuegra González, César
Martínez Serrano, Cristina Alicia
Hidalgo Prieto, Manuel
Dorado Martín, Jesús M.
Martínez Pastor, Felipe
Álvarez Rodríguez, Manuel
Rodríguez Martínez, Heriberto
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biologia Celular
Facultad de Ciencias Biologicas y Ambientales
dc.subject.none.fl_str_mv Biología
Veterinaria
Calcium channels
CatSper subunits
TRPC
Pig
Capacitation
2407 Biología Celular
3104.11 Reproducción
topic Biología
Veterinaria
Calcium channels
CatSper subunits
TRPC
Pig
Capacitation
2407 Biología Celular
3104.11 Reproducción
description [EN] Sperm capacitation is a stepwise complex biochemical process towards fertilization. It in-cludes a crucial early calcium (Ca2+) transport mediated by CatSper channels and Canonical Transient Potential Channels (TRPC). We studied the relative abundance of mRNA transcripts changes of the CatSper β, γ and δ subunits and TRPC-channels 1, 3, 4, 6 and 7 in pig spermatozoa, after trigger-ing in vitro capacitation by bicarbonate ions at levels present in vivo at the fertilization site. For this purpose, we analyzedfive5 ejaculate pools (from three fertile adult boars) before (control-fresh sam-ples) and after in vitro exposure to capacitation conditions (37 mM NaHCO3, 2.25 mM CaCl2, 2 mM caffeine, 0.5% bovine serum albumin and 310 mM lactose) at 38 °C, 5% CO2 for 30 min. In vitro capacitation using bicarbonate elicits an increase in the relative abundance of mRNA transcripts of almost all studied Ca2+ channels, except CatSper-δ and TRPC1 (significantly reduced). These findings open new avenues of research to identify the specific role of each channel in boar sperm capacitation and elucidate the physiological meaning of the changes on sperm mRNA cargo
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.mdpi.com/2076-2615/12/8/1012
https://hdl.handle.net/10612/21322
url https://www.mdpi.com/2076-2615/12/8/1012
https://hdl.handle.net/10612/21322
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/Swedish Research Council FORMAS, Stockholm Sweden//Projects 2017-00946 and 2019-00288
info:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/PID2019-108320RJ-I00
info:eu-repo/grantAgreement/MINECO/Programa Estatal de Promoción del Talento y su Empleabilidad/IJCI-2015-24380
info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018-095183-B-I00
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300724

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