Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2

13 páginas, 7 figuras

Detalles Bibliográficos
Autores: Pascual-García, Pau, Govind, Chhabi K ., Queralt, Ethel, Cuenca-Bono, Bernardo, Llopis, Ana, Chavez, Sebastián, Hinnebusch, Alan G., Rodríguez-Navarro, Susana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/345937
Acceso en línea:http://hdl.handle.net/10261/345937
Access Level:acceso abierto
Palabra clave:Sus1
SAGA
TREX2
Transcription elongation
mRNA export
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spelling Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2Pascual-García, PauGovind, Chhabi K .Queralt, EthelCuenca-Bono, BernardoLlopis, AnaChavez, SebastiánHinnebusch, Alan G.Rodríguez-Navarro, SusanaSus1SAGATREX2Transcription elongationmRNA export13 páginas, 7 figurasGene transcription, RNA biogenesis, and mRNA transport constitute a complicated process essential for all eukaryotic cells. The transcription/export factor Sus1 plays a key role in coupling transcription activation with mRNA export, and it resides in both the SAGA and TREX2 complexes. Moreover, Sus1 is responsible for GAL1 gene gating at the nuclear periphery, which is important for its transcriptional status. Here, we show that Sus1 is required during transcription elongation and is associated with the elongating form of RNA Polymerase II (RNAP II) phosphorylated on Ser5 and Ser2 of the C-terminal domain (CTD). In addition, Sus1 copurifies with the essential mRNA export factors Yra1 and Mex67, which bind to the mRNA cotranscriptionally. Consistently, ChIP analysis reveals that Sus1 is present at coding regions dependent on transcription in a manner stimulated by Kin28-dependent CTD phosphorylation. Strikingly, eliminating the TREX2 component Sac3 or the SAGA subunit Ubp8 partially impairs Sus1 targeting to coding sequences and upstream activating sequences (UAS). We found, unexpectedly, that Sgf73 is necessary for association of Sus1 with both SAGA and TREX2, and that its absence dramatically reduces Sus1 occupancy of UAS and ORF sequences. Our results reveal that Sus1 plays a key role in coordinating gene transcription and mRNA export by working at the interface between the SAGA and TREX2 complexes during transcription elongation.S.C. is supported by MEC (BFU2007-67575-C03-02) and the Andalusian Government (CVI-271). This work was supported partly by the Intramural Research Program of the NIH. S.R.-N. is supported by the Ramón y Cajal program and funded by the MEC (BFU2005-06856) and the GV (ACOMP06/165 and ACOMP07/013). P.P.- G. and B.C.-B. are holders of predoctoral fellowships from the CIPF and MEC, respectivellyPeer reviewedCold Spring Harbor Laboratory PressMinisterio de Economía y Competitividad (España)Junta de AndalucíaNational Institutes of Health (US)Generalitat ValencianaQueralt, Ethel [0000-0003-0045-0039]Rodríguez-Navarro, Susana [0000-0001-7472-3111]202420242008info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/345937reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MEC//BFU2007-67575-C03-02https://dx.doi.org/10.1101/gad.483308Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3459372026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2
title Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2
spellingShingle Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2
Pascual-García, Pau
Sus1
SAGA
TREX2
Transcription elongation
mRNA export
title_short Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2
title_full Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2
title_fullStr Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2
title_full_unstemmed Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2
title_sort Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2
dc.creator.none.fl_str_mv Pascual-García, Pau
Govind, Chhabi K .
Queralt, Ethel
Cuenca-Bono, Bernardo
Llopis, Ana
Chavez, Sebastián
Hinnebusch, Alan G.
Rodríguez-Navarro, Susana
author Pascual-García, Pau
author_facet Pascual-García, Pau
Govind, Chhabi K .
Queralt, Ethel
Cuenca-Bono, Bernardo
Llopis, Ana
Chavez, Sebastián
Hinnebusch, Alan G.
Rodríguez-Navarro, Susana
author_role author
author2 Govind, Chhabi K .
Queralt, Ethel
Cuenca-Bono, Bernardo
Llopis, Ana
Chavez, Sebastián
Hinnebusch, Alan G.
Rodríguez-Navarro, Susana
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Junta de Andalucía
National Institutes of Health (US)
Generalitat Valenciana
Queralt, Ethel [0000-0003-0045-0039]
Rodríguez-Navarro, Susana [0000-0001-7472-3111]
dc.subject.none.fl_str_mv Sus1
SAGA
TREX2
Transcription elongation
mRNA export
topic Sus1
SAGA
TREX2
Transcription elongation
mRNA export
description 13 páginas, 7 figuras
publishDate 2008
dc.date.none.fl_str_mv 2008
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/345937
url http://hdl.handle.net/10261/345937
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MEC//BFU2007-67575-C03-02
https://dx.doi.org/10.1101/gad.483308
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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