Biofunctionalization of cork with moringa oleifera seeds and use of pma staining and qpcr to detect viability of escherichia coli

Cork matrices biofunctionalized with Moringa oleifera seed extracts (MoSe) have potential for use as a biofilter with antibacterial properties to reduce waterborne pathogens. The aim of this study was to evaluate the effect of cork biofunctionalized with active antimicrobial compounds of MoSe (f-cor...

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Autores: Infante, Nury, Rodríguez, Refugio, Bartolo, Yaneth, Sánchez Martinez, Olga, Sanz Sáez, Isabel, Bermeo, Lizeth, Morató Farreras, Jordi|||0000-0003-2588-8846
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/359099
Acceso en línea:https://hdl.handle.net/2117/359099
https://dx.doi.org/10.3390/w13192731
Access Level:acceso abierto
Palabra clave:Escherichia coli
Water -- Purification
Cork-based biofilter
Viability qPCR
Lac Z gene
Propidium monoazide
Disinfection
Antimicrobial activity
Escheríchia coli
Aigua -- Depuració
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Ciències de la terra i de la vida::Microbiologia
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repository_id_str
spelling Biofunctionalization of cork with moringa oleifera seeds and use of pma staining and qpcr to detect viability of escherichia coliInfante, NuryRodríguez, RefugioBartolo, YanethSánchez Martinez, OlgaSanz Sáez, IsabelBermeo, LizethMorató Farreras, Jordi|||0000-0003-2588-8846Escherichia coliWater -- PurificationCork-based biofilterEscherichia coliViability qPCRLac Z genePropidium monoazideDisinfectionAntimicrobial activityEscheríchia coliAigua -- DepuracióÀrees temàtiques de la UPC::Enginyeria agroalimentària::Ciències de la terra i de la vida::MicrobiologiaCork matrices biofunctionalized with Moringa oleifera seed extracts (MoSe) have potential for use as a biofilter with antibacterial properties to reduce waterborne pathogens. The aim of this study was to evaluate the effect of cork biofunctionalized with active antimicrobial compounds of MoSe (f-cork) on the inhibition of Escherichia coli (InhEc). The LacZ gene from a strain of E. coli was used as the target sequence using viability quantification Polymerase Chain Reaction (qPCR) and differentiation of viable and dead bacteria through selective cell viability PMA staining. To perform this, a 27-4 fractional factorial design and a biofiltration system were used to evaluate the effect of the active protein in MoSe immobilized in granulated cork on InhEc. We found that the potential for antimicrobial activity increased with f-cork for an effective maximal bacterial reduction (99.99%; p < 0.05). The effect of f-cork functionalized with MoSe on E. coli viability was of 0.024% and 0.005% for the cells exposed to PMA, respectively, being the relevant conditions in treatment 2: (0 L/min) without aeration, (5%) MoSe and (5 mm) cork particle. In conclusion, the f-cork functionalized with MoSe presented biosorbent and antibacterial properties that effectively reduced the E. coli growth.This research was funded by Mexico-CONACYT, grant number 3006060 and grant by the UNESCO Chair on Sustainability of the Polytechnic University of Catalonia (Spain).Peer Reviewed20212021-10-0120212021-12-23journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/359099https://dx.doi.org/10.3390/w13192731reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 3.0 Spainhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3590992026-05-27T15:37:01Z
dc.title.none.fl_str_mv Biofunctionalization of cork with moringa oleifera seeds and use of pma staining and qpcr to detect viability of escherichia coli
title Biofunctionalization of cork with moringa oleifera seeds and use of pma staining and qpcr to detect viability of escherichia coli
spellingShingle Biofunctionalization of cork with moringa oleifera seeds and use of pma staining and qpcr to detect viability of escherichia coli
Infante, Nury
Escherichia coli
Water -- Purification
Cork-based biofilter
Escherichia coli
Viability qPCR
Lac Z gene
Propidium monoazide
Disinfection
Antimicrobial activity
Escheríchia coli
Aigua -- Depuració
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Ciències de la terra i de la vida::Microbiologia
title_short Biofunctionalization of cork with moringa oleifera seeds and use of pma staining and qpcr to detect viability of escherichia coli
title_full Biofunctionalization of cork with moringa oleifera seeds and use of pma staining and qpcr to detect viability of escherichia coli
title_fullStr Biofunctionalization of cork with moringa oleifera seeds and use of pma staining and qpcr to detect viability of escherichia coli
title_full_unstemmed Biofunctionalization of cork with moringa oleifera seeds and use of pma staining and qpcr to detect viability of escherichia coli
title_sort Biofunctionalization of cork with moringa oleifera seeds and use of pma staining and qpcr to detect viability of escherichia coli
dc.creator.none.fl_str_mv Infante, Nury
Rodríguez, Refugio
Bartolo, Yaneth
Sánchez Martinez, Olga
Sanz Sáez, Isabel
Bermeo, Lizeth
Morató Farreras, Jordi|||0000-0003-2588-8846
author Infante, Nury
author_facet Infante, Nury
Rodríguez, Refugio
Bartolo, Yaneth
Sánchez Martinez, Olga
Sanz Sáez, Isabel
Bermeo, Lizeth
Morató Farreras, Jordi|||0000-0003-2588-8846
author_role author
author2 Rodríguez, Refugio
Bartolo, Yaneth
Sánchez Martinez, Olga
Sanz Sáez, Isabel
Bermeo, Lizeth
Morató Farreras, Jordi|||0000-0003-2588-8846
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Escherichia coli
Water -- Purification
Cork-based biofilter
Escherichia coli
Viability qPCR
Lac Z gene
Propidium monoazide
Disinfection
Antimicrobial activity
Escheríchia coli
Aigua -- Depuració
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Ciències de la terra i de la vida::Microbiologia
topic Escherichia coli
Water -- Purification
Cork-based biofilter
Escherichia coli
Viability qPCR
Lac Z gene
Propidium monoazide
Disinfection
Antimicrobial activity
Escheríchia coli
Aigua -- Depuració
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Ciències de la terra i de la vida::Microbiologia
description Cork matrices biofunctionalized with Moringa oleifera seed extracts (MoSe) have potential for use as a biofilter with antibacterial properties to reduce waterborne pathogens. The aim of this study was to evaluate the effect of cork biofunctionalized with active antimicrobial compounds of MoSe (f-cork) on the inhibition of Escherichia coli (InhEc). The LacZ gene from a strain of E. coli was used as the target sequence using viability quantification Polymerase Chain Reaction (qPCR) and differentiation of viable and dead bacteria through selective cell viability PMA staining. To perform this, a 27-4 fractional factorial design and a biofiltration system were used to evaluate the effect of the active protein in MoSe immobilized in granulated cork on InhEc. We found that the potential for antimicrobial activity increased with f-cork for an effective maximal bacterial reduction (99.99%; p < 0.05). The effect of f-cork functionalized with MoSe on E. coli viability was of 0.024% and 0.005% for the cells exposed to PMA, respectively, being the relevant conditions in treatment 2: (0 L/min) without aeration, (5%) MoSe and (5 mm) cork particle. In conclusion, the f-cork functionalized with MoSe presented biosorbent and antibacterial properties that effectively reduced the E. coli growth.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-10-01
2021
2021-12-23
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/359099
https://dx.doi.org/10.3390/w13192731
url https://hdl.handle.net/2117/359099
https://dx.doi.org/10.3390/w13192731
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 3.0 Spain
http://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 3.0 Spain
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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