Encapsulation of Lavandula austroapennina N.G. Passal., Tundis & Upson extracts: Focus on leaf and stem enriched liposome for cosmeceutical innovation

In the framework aimed at valorizing Lavandula austroapennina N.G. Passal., Tundis & Upson, an endemic species of the Apennine mountains of southern Italy, through the deep investigation of its organ chemical composition and bioactivity, innovative cosmeceutical applications are of interest. Thu...

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Detalles Bibliográficos
Autores: Gravina, Claudia, Piccolella, Simona, Alonso, Cristina, Martí, Meritxell, Formato, Marialuisa, Pacifico, Severina, Coderch Negra, M. Luisa, Esposito, Assunta
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/352600
Acceso en línea:http://hdl.handle.net/10261/352600
https://api.elsevier.com/content/abstract/scopus_id/85188534265
Access Level:acceso embargado
Palabra clave:UHPLC-QqTOF-HR-MS
ATR-FTIR
Cosmeceuticals
Encapsulation
Lavandula austroapennina
Percutaneous absorption
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
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spelling Encapsulation of Lavandula austroapennina N.G. Passal., Tundis & Upson extracts: Focus on leaf and stem enriched liposome for cosmeceutical innovationGravina, ClaudiaPiccolella, SimonaAlonso, CristinaMartí, MeritxellFormato, MarialuisaPacifico, SeverinaCoderch Negra, M. LuisaEsposito, AssuntaUHPLC-QqTOF-HR-MSATR-FTIRCosmeceuticalsEncapsulationLavandula austroapenninaPercutaneous absorptionhttp://metadata.un.org/sdg/3Ensure healthy lives and promote well-being for all at all agesIn the framework aimed at valorizing Lavandula austroapennina N.G. Passal., Tundis & Upson, an endemic species of the Apennine mountains of southern Italy, through the deep investigation of its organ chemical composition and bioactivity, innovative cosmeceutical applications are of interest. Thus, lipophilic and alcoholic extracts, at dose levels avoiding cytotoxicity in keratinocytes, were encapsulated into liposomes by applying thin-film hydration method. This was aimed to overcome known plant extract issues, such as poor solubility, high instability, and low skin permeation. The lipid-based vesicles were characterized by particle size dimension ≤300 nm, negative charge (-8 to −20 mV) and polydispersity index <0.3. Entrapment efficiency distinguished liposomes encapsulating alcoholic extracts from corolla- and calyx for their highest flavonoids, while those with leaf- and root alcoholic extracts excelled in phenolic acids and their conjugates. Among lipophilic extracts, those from corolla, calyx and stem provided liposomes entrapping the highest lipid content. These data, together with the greater bioactivity of alcoholic leaf, and lipophilic stem extract, allowed us to further investigate these two extracts for their cosmeceutical applicability. Accordingly, extracts-containing liposomes, preliminarily characterized by ATR-FTIR spectroscopy, underwent cytotoxic and healing capability assessment towards HaCaT cells. The extracts-enriched liposomes showed a healing capacity higher than the parental extracts from which they derived. Evaluating the percutaneous absorption by Franz Cells assay, followed by UHPLC-QqTOF-HR-MS-based quali-quantitative analysis, revealed that encapsulation in liposomes improved bioactive delivery through/into a specific skin layer. These results, while suggesting the effectiveness of liposome-based nanoparticles in improving the bioavailability and biological activity of specialized metabolites, reinforce the cosmeceutical use of L. austroapennina extracts.Peer reviewedElsevierConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/352600https://api.elsevier.com/content/abstract/scopus_id/85188534265reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésIndustrial Crops and Productshttps://doi.org/10.1016/j.indcrop.2024.118362Síinfo:eu-repo/semantics/embargoedAccessoai:digital.csic.es:10261/3526002026-05-22T06:33:51Z
dc.title.none.fl_str_mv Encapsulation of Lavandula austroapennina N.G. Passal., Tundis & Upson extracts: Focus on leaf and stem enriched liposome for cosmeceutical innovation
title Encapsulation of Lavandula austroapennina N.G. Passal., Tundis & Upson extracts: Focus on leaf and stem enriched liposome for cosmeceutical innovation
spellingShingle Encapsulation of Lavandula austroapennina N.G. Passal., Tundis & Upson extracts: Focus on leaf and stem enriched liposome for cosmeceutical innovation
Gravina, Claudia
UHPLC-QqTOF-HR-MS
ATR-FTIR
Cosmeceuticals
Encapsulation
Lavandula austroapennina
Percutaneous absorption
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
title_short Encapsulation of Lavandula austroapennina N.G. Passal., Tundis & Upson extracts: Focus on leaf and stem enriched liposome for cosmeceutical innovation
title_full Encapsulation of Lavandula austroapennina N.G. Passal., Tundis & Upson extracts: Focus on leaf and stem enriched liposome for cosmeceutical innovation
title_fullStr Encapsulation of Lavandula austroapennina N.G. Passal., Tundis & Upson extracts: Focus on leaf and stem enriched liposome for cosmeceutical innovation
title_full_unstemmed Encapsulation of Lavandula austroapennina N.G. Passal., Tundis & Upson extracts: Focus on leaf and stem enriched liposome for cosmeceutical innovation
title_sort Encapsulation of Lavandula austroapennina N.G. Passal., Tundis & Upson extracts: Focus on leaf and stem enriched liposome for cosmeceutical innovation
dc.creator.none.fl_str_mv Gravina, Claudia
Piccolella, Simona
Alonso, Cristina
Martí, Meritxell
Formato, Marialuisa
Pacifico, Severina
Coderch Negra, M. Luisa
Esposito, Assunta
author Gravina, Claudia
author_facet Gravina, Claudia
Piccolella, Simona
Alonso, Cristina
Martí, Meritxell
Formato, Marialuisa
Pacifico, Severina
Coderch Negra, M. Luisa
Esposito, Assunta
author_role author
author2 Piccolella, Simona
Alonso, Cristina
Martí, Meritxell
Formato, Marialuisa
Pacifico, Severina
Coderch Negra, M. Luisa
Esposito, Assunta
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv UHPLC-QqTOF-HR-MS
ATR-FTIR
Cosmeceuticals
Encapsulation
Lavandula austroapennina
Percutaneous absorption
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
topic UHPLC-QqTOF-HR-MS
ATR-FTIR
Cosmeceuticals
Encapsulation
Lavandula austroapennina
Percutaneous absorption
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
description In the framework aimed at valorizing Lavandula austroapennina N.G. Passal., Tundis & Upson, an endemic species of the Apennine mountains of southern Italy, through the deep investigation of its organ chemical composition and bioactivity, innovative cosmeceutical applications are of interest. Thus, lipophilic and alcoholic extracts, at dose levels avoiding cytotoxicity in keratinocytes, were encapsulated into liposomes by applying thin-film hydration method. This was aimed to overcome known plant extract issues, such as poor solubility, high instability, and low skin permeation. The lipid-based vesicles were characterized by particle size dimension ≤300 nm, negative charge (-8 to −20 mV) and polydispersity index <0.3. Entrapment efficiency distinguished liposomes encapsulating alcoholic extracts from corolla- and calyx for their highest flavonoids, while those with leaf- and root alcoholic extracts excelled in phenolic acids and their conjugates. Among lipophilic extracts, those from corolla, calyx and stem provided liposomes entrapping the highest lipid content. These data, together with the greater bioactivity of alcoholic leaf, and lipophilic stem extract, allowed us to further investigate these two extracts for their cosmeceutical applicability. Accordingly, extracts-containing liposomes, preliminarily characterized by ATR-FTIR spectroscopy, underwent cytotoxic and healing capability assessment towards HaCaT cells. The extracts-enriched liposomes showed a healing capacity higher than the parental extracts from which they derived. Evaluating the percutaneous absorption by Franz Cells assay, followed by UHPLC-QqTOF-HR-MS-based quali-quantitative analysis, revealed that encapsulation in liposomes improved bioactive delivery through/into a specific skin layer. These results, while suggesting the effectiveness of liposome-based nanoparticles in improving the bioavailability and biological activity of specialized metabolites, reinforce the cosmeceutical use of L. austroapennina extracts.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/352600
https://api.elsevier.com/content/abstract/scopus_id/85188534265
url http://hdl.handle.net/10261/352600
https://api.elsevier.com/content/abstract/scopus_id/85188534265
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Industrial Crops and Products
https://doi.org/10.1016/j.indcrop.2024.118362
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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