Quantitative analysis of the corneal collagen distribution after in vivo cross-linking with second harmonic microscopy

Producción Científica

Detalles Bibliográficos
Autores: Bueno, Juan M., Ávila, Francisco J., Martínez García, María del Carmen
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/45092
Acceso en línea:https://doi.org/10.1155/2019/3860498
http://uvadoc.uva.es/handle/10324/45092
Access Level:acceso abierto
Palabra clave:Cross-linking technique
Técnica de cross-linking
Corneal collagen
Colágeno corneal
2209.15 Optometría
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spelling Quantitative analysis of the corneal collagen distribution after in vivo cross-linking with second harmonic microscopyBueno, Juan M.Ávila, Francisco J.Martínez García, María del CarmenCross-linking techniqueTécnica de cross-linkingCorneal collagenColágeno corneal2209.15 OptometríaProducción CientíficaCorneal cross-linking (CXL) is a surgical procedure able to modify corneal biomechanics and stabilize keratoconus progression. Although it is known that CXL produces changes in corneal collagen distribution, these are still a topic of discussion. Here we quantitatively compare the corneal stroma architecture between two animal models four weeks after in vivo conventional CXL treatment, with second harmonic generation (SHG) imaging microscopy and the structure tensor (ST). The healing stage and the stroma recovery were also analyzed by means of histological sections. Results show that the CXL effects depend on the initial arrangement of the corneal collagen. While the treatment increases the order in corneas with a low level of initial organization, corneas presenting a fairly regular pattern are hardly affected. Histological samples showed active keratocytes in anterior and middle stroma, what means that the recovery is still in progress. The combination of SHG imaging and the ST is able to objectively discriminate the changes suffered by the collagen arrangement after the CXL treatment, whose effectiveness depends on the initial organization of the collagen fibers within the corneal stroma.Sociedad Española para la Investigación de las Diferencias Individuales (grant FIS2016-76163-R)Fundación Séneca (grant 19897/GERM/15)Hindawi2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1155/2019/3860498http://uvadoc.uva.es/handle/10324/45092reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://www.hindawi.com/journals/bmri/2019/3860498/info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:uvadoc.uva.es:10324/450922026-06-13T12:44:47Z
dc.title.none.fl_str_mv Quantitative analysis of the corneal collagen distribution after in vivo cross-linking with second harmonic microscopy
title Quantitative analysis of the corneal collagen distribution after in vivo cross-linking with second harmonic microscopy
spellingShingle Quantitative analysis of the corneal collagen distribution after in vivo cross-linking with second harmonic microscopy
Bueno, Juan M.
Cross-linking technique
Técnica de cross-linking
Corneal collagen
Colágeno corneal
2209.15 Optometría
title_short Quantitative analysis of the corneal collagen distribution after in vivo cross-linking with second harmonic microscopy
title_full Quantitative analysis of the corneal collagen distribution after in vivo cross-linking with second harmonic microscopy
title_fullStr Quantitative analysis of the corneal collagen distribution after in vivo cross-linking with second harmonic microscopy
title_full_unstemmed Quantitative analysis of the corneal collagen distribution after in vivo cross-linking with second harmonic microscopy
title_sort Quantitative analysis of the corneal collagen distribution after in vivo cross-linking with second harmonic microscopy
dc.creator.none.fl_str_mv Bueno, Juan M.
Ávila, Francisco J.
Martínez García, María del Carmen
author Bueno, Juan M.
author_facet Bueno, Juan M.
Ávila, Francisco J.
Martínez García, María del Carmen
author_role author
author2 Ávila, Francisco J.
Martínez García, María del Carmen
author2_role author
author
dc.subject.none.fl_str_mv Cross-linking technique
Técnica de cross-linking
Corneal collagen
Colágeno corneal
2209.15 Optometría
topic Cross-linking technique
Técnica de cross-linking
Corneal collagen
Colágeno corneal
2209.15 Optometría
description Producción Científica
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1155/2019/3860498
http://uvadoc.uva.es/handle/10324/45092
url https://doi.org/10.1155/2019/3860498
http://uvadoc.uva.es/handle/10324/45092
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.hindawi.com/journals/bmri/2019/3860498/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi
publisher.none.fl_str_mv Hindawi
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
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