1H Nuclear Magnetic Resonance of Pig Seminal Plasma Reveals Intra-Ejaculate Variation in Metabolites

Este artículo contiene 16 páginas, 5 figuras, 1 tabla.

Detalles Bibliográficos
Autores: Mateo-Otero, Yentel, Fernández-López, Pol, Gil-Caballero, Sergio, Fernández-Fuertes, Beatriz, Bonet, Sergi, Barranco, Isabel, Yeste, Marc
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/215417
Acceso en línea:http://hdl.handle.net/10261/215417
Access Level:acceso abierto
Palabra clave:Pig
Intra-ejaculate variability
Nuclear magnetic resonance
Seminal plasma
Metabolites
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spelling 1H Nuclear Magnetic Resonance of Pig Seminal Plasma Reveals Intra-Ejaculate Variation in MetabolitesMateo-Otero, YentelFernández-López, PolGil-Caballero, SergioFernández-Fuertes, BeatrizBonet, SergiBarranco, IsabelYeste, MarcPigIntra-ejaculate variabilityNuclear magnetic resonanceSeminal plasmaMetabolitesEste artículo contiene 16 páginas, 5 figuras, 1 tabla.In pigs, ejaculate is expelled in fractions, mainly the sperm-rich fraction (SRF) and the post-SRF (PSRF), which differ in both sperm content and origin. In addition, intra-ejaculate variability between fractions in terms of sperm reproductive characteristics has been previously reported, the highest sperm quality being observed in the first 10 mL of the SRF (SRF-P1). As seminal plasma (SP) composition has been purported to influence sperm physiology, the aim of this study was to profile pig SP metabolite composition and to find putative differences between the ejaculate portions (SRF-P1, the rest of SRF [SRF-P2], PSRF) and entire ejaculate (EE). To this end, ejaculates (n = 8, one per boar) were collected in fractions and SP was analyzed using 1H Nuclear Magnetic Resonance spectroscopy. We identified 19 metabolites present in all ejaculate portions and the EE, and reported correlations between the metabolites. Additionally, and for the first time in mammals, we found intra-ejaculate variability in the SP metabolites, observing different relative abundances in choline, glycerophosphocholine and glycine. Regarding their influence in sperm physiology, we hypothesize that these metabolites may explain the specific reproductive characteristics of each ejaculate portion. Finally, the reported SP metabolites could serve as a first steppingstone in the study of quality, functionality, and fertility biomarkersThe present study has been funded by the Ministry of Science and Innovation, Spain (Grants: RYC-2014-15581, AGL2017-88329-R and FJCI-2017-31689) and the Regional Government of Catalonia (Grants: 2019-SGR-1229 and 2020-FI-B-00412).Peer reviewedMultidisciplinary Digital Publishing InstituteConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/215417reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/biom10060906Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2154172026-05-22T06:33:51Z
dc.title.none.fl_str_mv 1H Nuclear Magnetic Resonance of Pig Seminal Plasma Reveals Intra-Ejaculate Variation in Metabolites
title 1H Nuclear Magnetic Resonance of Pig Seminal Plasma Reveals Intra-Ejaculate Variation in Metabolites
spellingShingle 1H Nuclear Magnetic Resonance of Pig Seminal Plasma Reveals Intra-Ejaculate Variation in Metabolites
Mateo-Otero, Yentel
Pig
Intra-ejaculate variability
Nuclear magnetic resonance
Seminal plasma
Metabolites
title_short 1H Nuclear Magnetic Resonance of Pig Seminal Plasma Reveals Intra-Ejaculate Variation in Metabolites
title_full 1H Nuclear Magnetic Resonance of Pig Seminal Plasma Reveals Intra-Ejaculate Variation in Metabolites
title_fullStr 1H Nuclear Magnetic Resonance of Pig Seminal Plasma Reveals Intra-Ejaculate Variation in Metabolites
title_full_unstemmed 1H Nuclear Magnetic Resonance of Pig Seminal Plasma Reveals Intra-Ejaculate Variation in Metabolites
title_sort 1H Nuclear Magnetic Resonance of Pig Seminal Plasma Reveals Intra-Ejaculate Variation in Metabolites
dc.creator.none.fl_str_mv Mateo-Otero, Yentel
Fernández-López, Pol
Gil-Caballero, Sergio
Fernández-Fuertes, Beatriz
Bonet, Sergi
Barranco, Isabel
Yeste, Marc
author Mateo-Otero, Yentel
author_facet Mateo-Otero, Yentel
Fernández-López, Pol
Gil-Caballero, Sergio
Fernández-Fuertes, Beatriz
Bonet, Sergi
Barranco, Isabel
Yeste, Marc
author_role author
author2 Fernández-López, Pol
Gil-Caballero, Sergio
Fernández-Fuertes, Beatriz
Bonet, Sergi
Barranco, Isabel
Yeste, Marc
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Pig
Intra-ejaculate variability
Nuclear magnetic resonance
Seminal plasma
Metabolites
topic Pig
Intra-ejaculate variability
Nuclear magnetic resonance
Seminal plasma
Metabolites
description Este artículo contiene 16 páginas, 5 figuras, 1 tabla.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/215417
url http://hdl.handle.net/10261/215417
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/biom10060906
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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