Characterization of the interferon pathway in the teleost fish gonad against the vertically transmitted viral nervous necrosis virus

One of the most powerful innate immune responses against viruses is mediated by type I IFN. In teleost fish, it is known that virus infection triggers the expression of ifn and many IFN-stimulated genes, but the viral RNA sensors and mediators leading to IFN production are scarcely known. Thus, we h...

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Autores: Valero, Yulema, Morcillo, Patricia, Meseguer, José, Buonocore, Francesco, Esteban, María Ángeles, Chaves-Pozo, Elena, Cuesta, Alberto
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/313860
Acceso en línea:http://hdl.handle.net/10261/313860
Access Level:acceso abierto
Palabra clave:Nodavirus (VNNV)
Acuicultura
Centro Oceanográfico de Murcia
interferon (IFN) pathway
gonad
Gilthead seabream
European sea bass
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spelling Characterization of the interferon pathway in the teleost fish gonad against the vertically transmitted viral nervous necrosis virusValero, YulemaMorcillo, PatriciaMeseguer, JoséBuonocore, FrancescoEsteban, María ÁngelesChaves-Pozo, ElenaCuesta, AlbertoNodavirus (VNNV)AcuiculturaCentro Oceanográfico de Murciainterferon (IFN) pathwaygonadGilthead seabreamEuropean sea bassOne of the most powerful innate immune responses against viruses is mediated by type I IFN. In teleost fish, it is known that virus infection triggers the expression of ifn and many IFN-stimulated genes, but the viral RNA sensors and mediators leading to IFN production are scarcely known. Thus, we have searched for the presence of these genes in gilt-head sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax), and evaluated their expression after infection with viral nervous necrosis virus (VNNV) in the brain, the main viral target tissue, and the gonad, used to transmit the virus vertically. In sea bream, a fish species resistant to the VNNV strain used, we found an upregulation of the genes encoding MDA5 (melanoma differentiation-associated gene 5), TBK1 (TANK-binding kinase 1), IRF3 (IFN regulatory factor 3), IFN, Mx [myxovirus (influenza) resistance protein] and PKR (dsRNA-dependent protein kinase receptor) proteins in the brain, which were unaltered in the gonad and could favour the dissemination by gonad fluids or gametes. Strikingly, in European sea bass, a very susceptible species, we also identified, transcripts coding for LGP2 (Laboratory of Genetics and Physiology 2), MAVS (mitochondrial antiviral signalling), TRAF3 (TNF receptor-associated factor 3), TANK (TRAF family member-associated NFkappaB activator) and IRF7 (IFN regulatory factor 7), and found that all the genes analysed were upregulated in the gonad, but only mda5, lgp2, irf3, mx and pkr were upregulated in the brain. These findings supported the notion that the European sea bass brain innate immune response is unable to clear the virus and pointed to the importance of gonad immunity to control the dissemination of VNNV to the progeny - an aspect that is worth investigating in aquatic animals.SI202320232015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/313860reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCentro Oceanográfico de Murciahttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=25918238info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3138602026-05-22T06:33:51Z
dc.title.none.fl_str_mv Characterization of the interferon pathway in the teleost fish gonad against the vertically transmitted viral nervous necrosis virus
title Characterization of the interferon pathway in the teleost fish gonad against the vertically transmitted viral nervous necrosis virus
spellingShingle Characterization of the interferon pathway in the teleost fish gonad against the vertically transmitted viral nervous necrosis virus
Valero, Yulema
Nodavirus (VNNV)
Acuicultura
Centro Oceanográfico de Murcia
interferon (IFN) pathway
gonad
Gilthead seabream
European sea bass
title_short Characterization of the interferon pathway in the teleost fish gonad against the vertically transmitted viral nervous necrosis virus
title_full Characterization of the interferon pathway in the teleost fish gonad against the vertically transmitted viral nervous necrosis virus
title_fullStr Characterization of the interferon pathway in the teleost fish gonad against the vertically transmitted viral nervous necrosis virus
title_full_unstemmed Characterization of the interferon pathway in the teleost fish gonad against the vertically transmitted viral nervous necrosis virus
title_sort Characterization of the interferon pathway in the teleost fish gonad against the vertically transmitted viral nervous necrosis virus
dc.creator.none.fl_str_mv Valero, Yulema
Morcillo, Patricia
Meseguer, José
Buonocore, Francesco
Esteban, María Ángeles
Chaves-Pozo, Elena
Cuesta, Alberto
author Valero, Yulema
author_facet Valero, Yulema
Morcillo, Patricia
Meseguer, José
Buonocore, Francesco
Esteban, María Ángeles
Chaves-Pozo, Elena
Cuesta, Alberto
author_role author
author2 Morcillo, Patricia
Meseguer, José
Buonocore, Francesco
Esteban, María Ángeles
Chaves-Pozo, Elena
Cuesta, Alberto
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Nodavirus (VNNV)
Acuicultura
Centro Oceanográfico de Murcia
interferon (IFN) pathway
gonad
Gilthead seabream
European sea bass
topic Nodavirus (VNNV)
Acuicultura
Centro Oceanográfico de Murcia
interferon (IFN) pathway
gonad
Gilthead seabream
European sea bass
description One of the most powerful innate immune responses against viruses is mediated by type I IFN. In teleost fish, it is known that virus infection triggers the expression of ifn and many IFN-stimulated genes, but the viral RNA sensors and mediators leading to IFN production are scarcely known. Thus, we have searched for the presence of these genes in gilt-head sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax), and evaluated their expression after infection with viral nervous necrosis virus (VNNV) in the brain, the main viral target tissue, and the gonad, used to transmit the virus vertically. In sea bream, a fish species resistant to the VNNV strain used, we found an upregulation of the genes encoding MDA5 (melanoma differentiation-associated gene 5), TBK1 (TANK-binding kinase 1), IRF3 (IFN regulatory factor 3), IFN, Mx [myxovirus (influenza) resistance protein] and PKR (dsRNA-dependent protein kinase receptor) proteins in the brain, which were unaltered in the gonad and could favour the dissemination by gonad fluids or gametes. Strikingly, in European sea bass, a very susceptible species, we also identified, transcripts coding for LGP2 (Laboratory of Genetics and Physiology 2), MAVS (mitochondrial antiviral signalling), TRAF3 (TNF receptor-associated factor 3), TANK (TRAF family member-associated NFkappaB activator) and IRF7 (IFN regulatory factor 7), and found that all the genes analysed were upregulated in the gonad, but only mda5, lgp2, irf3, mx and pkr were upregulated in the brain. These findings supported the notion that the European sea bass brain innate immune response is unable to clear the virus and pointed to the importance of gonad immunity to control the dissemination of VNNV to the progeny - an aspect that is worth investigating in aquatic animals.
publishDate 2015
dc.date.none.fl_str_mv 2015
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/313860
url http://hdl.handle.net/10261/313860
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Centro Oceanográfico de Murcia
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=25918238
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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