Expression of the biofilm-associated protein interferes with host protein receptors of Staphylococcus aureus and alters the infective process

The adherence of Staphylococcus aureus to soluble proteins and extracellular-matrix components of the host is one of the key steps in the pathogenesis of staphylococcal infections. S. aureus presents a family of adhesins called MSCRAMMs (microbial surface components recognizing adhesive matrix molec...

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Autores: Cucarella, Carme, Tormo Más, María Ángeles, Knecht, Erwin, Amorena Zabalza, Beatriz, Lasa Uzcudun, Íñigo, Foster, Timothy J., Penadés, José R.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2002
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/32082
Acceso en línea:https://hdl.handle.net/2454/32082
Access Level:acceso abierto
Palabra clave:Biofilms
Staphylococcus aureus
Carrier Proteins
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spelling Expression of the biofilm-associated protein interferes with host protein receptors of Staphylococcus aureus and alters the infective processCucarella, CarmeTormo Más, María ÁngelesKnecht, ErwinAmorena Zabalza, BeatrizLasa Uzcudun, ÍñigoFoster, Timothy J.Penadés, José R.BiofilmsStaphylococcus aureusCarrier ProteinsThe adherence of Staphylococcus aureus to soluble proteins and extracellular-matrix components of the host is one of the key steps in the pathogenesis of staphylococcal infections. S. aureus presents a family of adhesins called MSCRAMMs (microbial surface components recognizing adhesive matrix molecules) that specifically recognize host matrix components. We examined the influence of biofilm-associated protein (Bap) expression on S. aureus adherence to host proteins, epithelial cell cultures, and mammary gland sections and on colonization of the mammary gland in an in vivo infection model. Bap-positive strain V329 showed lower adherence to immobilized fibrinogen and fibronectin than isogenic Bap-deficient strain m556. Bacterial adherence to histological sections of mammary gland and bacterial internalization into 293 cells were significantly lower in the Bap-positive strains. In addition, the Bap-negative strain showed significantly higher colonization in vivo of sheep mammary glands than the Bap-positive strain. Taken together, these results strongly suggest that the expression of the Bap protein interferes with functional properties of the MSCRAMM proteins, preventing initial bacterial attachment to host tissues and cellular internalization.This work was supported by grant BIO99-0285 from the Comisión Interministerial de Ciencia y Tecnología (C.I.C.Y.T.) and grants from the Cardenal Herrera-CEU University to J.R.P. and from the Wellcome Trust to T.J.F. Fellowship support for C. Cucarella and M. Á. Tormo from the Cardenal Herrera-CEU University is gratefully acknowledged. J. R. Penadés was partially supported by the “Conselleria de Cultura, Educació i Ciència” of the Generalitat Valenciana (Spain).American Society for MicrobiologyIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua2002info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/32082reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglés© 2002, American Society for Microbiology. All Rights Reserved.info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/320822026-06-17T12:41:47Z
dc.title.none.fl_str_mv Expression of the biofilm-associated protein interferes with host protein receptors of Staphylococcus aureus and alters the infective process
title Expression of the biofilm-associated protein interferes with host protein receptors of Staphylococcus aureus and alters the infective process
spellingShingle Expression of the biofilm-associated protein interferes with host protein receptors of Staphylococcus aureus and alters the infective process
Cucarella, Carme
Biofilms
Staphylococcus aureus
Carrier Proteins
title_short Expression of the biofilm-associated protein interferes with host protein receptors of Staphylococcus aureus and alters the infective process
title_full Expression of the biofilm-associated protein interferes with host protein receptors of Staphylococcus aureus and alters the infective process
title_fullStr Expression of the biofilm-associated protein interferes with host protein receptors of Staphylococcus aureus and alters the infective process
title_full_unstemmed Expression of the biofilm-associated protein interferes with host protein receptors of Staphylococcus aureus and alters the infective process
title_sort Expression of the biofilm-associated protein interferes with host protein receptors of Staphylococcus aureus and alters the infective process
dc.creator.none.fl_str_mv Cucarella, Carme
Tormo Más, María Ángeles
Knecht, Erwin
Amorena Zabalza, Beatriz
Lasa Uzcudun, Íñigo
Foster, Timothy J.
Penadés, José R.
author Cucarella, Carme
author_facet Cucarella, Carme
Tormo Más, María Ángeles
Knecht, Erwin
Amorena Zabalza, Beatriz
Lasa Uzcudun, Íñigo
Foster, Timothy J.
Penadés, José R.
author_role author
author2 Tormo Más, María Ángeles
Knecht, Erwin
Amorena Zabalza, Beatriz
Lasa Uzcudun, Íñigo
Foster, Timothy J.
Penadés, José R.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
dc.subject.none.fl_str_mv Biofilms
Staphylococcus aureus
Carrier Proteins
topic Biofilms
Staphylococcus aureus
Carrier Proteins
description The adherence of Staphylococcus aureus to soluble proteins and extracellular-matrix components of the host is one of the key steps in the pathogenesis of staphylococcal infections. S. aureus presents a family of adhesins called MSCRAMMs (microbial surface components recognizing adhesive matrix molecules) that specifically recognize host matrix components. We examined the influence of biofilm-associated protein (Bap) expression on S. aureus adherence to host proteins, epithelial cell cultures, and mammary gland sections and on colonization of the mammary gland in an in vivo infection model. Bap-positive strain V329 showed lower adherence to immobilized fibrinogen and fibronectin than isogenic Bap-deficient strain m556. Bacterial adherence to histological sections of mammary gland and bacterial internalization into 293 cells were significantly lower in the Bap-positive strains. In addition, the Bap-negative strain showed significantly higher colonization in vivo of sheep mammary glands than the Bap-positive strain. Taken together, these results strongly suggest that the expression of the Bap protein interferes with functional properties of the MSCRAMM proteins, preventing initial bacterial attachment to host tissues and cellular internalization.
publishDate 2002
dc.date.none.fl_str_mv 2002
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/32082
url https://hdl.handle.net/2454/32082
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv © 2002, American Society for Microbiology. All Rights Reserved.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2002, American Society for Microbiology. All Rights Reserved.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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