Citrus exocortis viroid causes ribosomal stress in tomato plants

[EN] Viroids are naked RNAs that do not code for any known protein and yet are able to infect plants causing severe diseases. Because of their RNA nature, many studies have focused on the involvement of viroids in RNA-mediated gene silencing as being their pathogenesis mechanism. Here, the alteratio...

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Autores: Cottilli, Patrick, Adkar-Purushothama, Charith Raj, Perreault, Jean-Pierre, Schleiff, Enrico, Belda Palazón, Borja, Rodrigo Bravo, Ismael|||0000-0002-8994-6112, Ferrando Monleón, Alejandro Ramón|||0000-0002-1903-9111, Lisón, Purificación|||0000-0002-1662-8084
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/159849
Acceso en línea:https://riunet.upv.es/handle/10251/159849
Access Level:acceso abierto
Palabra clave:BIOQUIMICA Y BIOLOGIA MOLECULAR
id ES_70ebd609fa47eb7665dff048ddf1a2d4
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network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Citrus exocortis viroid causes ribosomal stress in tomato plants
title Citrus exocortis viroid causes ribosomal stress in tomato plants
spellingShingle Citrus exocortis viroid causes ribosomal stress in tomato plants
Cottilli, Patrick
BIOQUIMICA Y BIOLOGIA MOLECULAR
title_short Citrus exocortis viroid causes ribosomal stress in tomato plants
title_full Citrus exocortis viroid causes ribosomal stress in tomato plants
title_fullStr Citrus exocortis viroid causes ribosomal stress in tomato plants
title_full_unstemmed Citrus exocortis viroid causes ribosomal stress in tomato plants
title_sort Citrus exocortis viroid causes ribosomal stress in tomato plants
dc.creator.none.fl_str_mv Cottilli, Patrick
Adkar-Purushothama, Charith Raj
Perreault, Jean-Pierre
Schleiff, Enrico
Belda Palazón, Borja
Rodrigo Bravo, Ismael|||0000-0002-8994-6112
Ferrando Monleón, Alejandro Ramón|||0000-0002-1903-9111
Lisón, Purificación|||0000-0002-1662-8084
author Cottilli, Patrick
author_facet Cottilli, Patrick
Adkar-Purushothama, Charith Raj
Perreault, Jean-Pierre
Schleiff, Enrico
Belda Palazón, Borja
Rodrigo Bravo, Ismael|||0000-0002-8994-6112
Ferrando Monleón, Alejandro Ramón|||0000-0002-1903-9111
Lisón, Purificación|||0000-0002-1662-8084
author_role author
author2 Adkar-Purushothama, Charith Raj
Perreault, Jean-Pierre
Schleiff, Enrico
Belda Palazón, Borja
Rodrigo Bravo, Ismael|||0000-0002-8994-6112
Ferrando Monleón, Alejandro Ramón|||0000-0002-1903-9111
Lisón, Purificación|||0000-0002-1662-8084
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Departamento de Biotecnología
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Ministerio de Economía y Competitividad
Generalitat Valenciana
Deutsche Forschungsgemeinschaft
Natural Sciences and Engineering Research Council of Canada
Ministerio de Ciencia e Innovación
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv BIOQUIMICA Y BIOLOGIA MOLECULAR
topic BIOQUIMICA Y BIOLOGIA MOLECULAR
description [EN] Viroids are naked RNAs that do not code for any known protein and yet are able to infect plants causing severe diseases. Because of their RNA nature, many studies have focused on the involvement of viroids in RNA-mediated gene silencing as being their pathogenesis mechanism. Here, the alterations caused by the Citrus exocortis viroid (CEVd) on the tomato translation machinery were studied as a new aspect of viroid pathogenesis. The presence of viroids in the ribosomal fractions of infected tomato plants was detected. More precisely, CEVd and its derived viroid small RNAs were found to co-sediment with tomato ribosomes in vivo, and to provoke changes in the global polysome profiles, particularly in the 40S ribosomal subunit accumulation. Additionally, the viroid caused alterations in ribosome biogenesis in the infected tomato plants, affecting the 18S rRNA maturation process. A higher expression level of the ribosomal stress mediator NAC082 was also detected in the CEVd-infected tomato leaves. Both the alterations in the rRNA processing and the induction of NAC082 correlate with the degree of viroid symptomatology. Taken together, these results suggest that CEVd is responsible for defective ribosome biogenesis in tomato, thereby interfering with the translation machinery and, therefore, causing ribosomal stress.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-09-19
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/159849
url https://riunet.upv.es/handle/10251/159849
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BIO2009-11818 Hipusinacion Del Factor Eif5A Y Muerte Celular Inducida Por Estres En Plantas
Natural Sciences and Engineering Research Council of Canada NSERC 155219-17
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BIO2015-70483-R PAPEL DE LA ESPERMIDINA Y DE LA BIOSINTESIS DE PROTEINAS EN LA TOLERANCIA DEL POLEN A LAS ALTAS TEMPERATURAS
Generalitat Valenciana https://doi.org/10.13039/501100003359 APOSTD%2F2017%2F039
Deutsche Forschungsgemeinschaft https://doi.org/10.13039/501100001659 SFB 902
Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BFU2009-11958 Señalizacion Y Respuesta Defensiva De Las Plantas Frente A Patogenos
Generalitat Valenciana https://doi.org/10.13039/501100003359 AICO%2F2017%2F048
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial (by-nc)
http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial (by-nc)
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Citrus exocortis viroid causes ribosomal stress in tomato plantsCottilli, PatrickAdkar-Purushothama, Charith RajPerreault, Jean-PierreSchleiff, EnricoBelda Palazón, BorjaRodrigo Bravo, Ismael|||0000-0002-8994-6112Ferrando Monleón, Alejandro Ramón|||0000-0002-1903-9111Lisón, Purificación|||0000-0002-1662-8084BIOQUIMICA Y BIOLOGIA MOLECULAR[EN] Viroids are naked RNAs that do not code for any known protein and yet are able to infect plants causing severe diseases. Because of their RNA nature, many studies have focused on the involvement of viroids in RNA-mediated gene silencing as being their pathogenesis mechanism. Here, the alterations caused by the Citrus exocortis viroid (CEVd) on the tomato translation machinery were studied as a new aspect of viroid pathogenesis. The presence of viroids in the ribosomal fractions of infected tomato plants was detected. More precisely, CEVd and its derived viroid small RNAs were found to co-sediment with tomato ribosomes in vivo, and to provoke changes in the global polysome profiles, particularly in the 40S ribosomal subunit accumulation. Additionally, the viroid caused alterations in ribosome biogenesis in the infected tomato plants, affecting the 18S rRNA maturation process. A higher expression level of the ribosomal stress mediator NAC082 was also detected in the CEVd-infected tomato leaves. Both the alterations in the rRNA processing and the induction of NAC082 correlate with the degree of viroid symptomatology. Taken together, these results suggest that CEVd is responsible for defective ribosome biogenesis in tomato, thereby interfering with the translation machinery and, therefore, causing ribosomal stress.Spanish Ministry of Science, Innovation and Universities [BIO2009-11818, BIO2015-70483-R to A.F.]; Spanish Ministry of Science, Innovation and Universities [BFU2009-11958]; Generalitat Valenciana (Valencia, Spain) [AICO/2017/048]; Natural Sciences and Engineering Research Council of Canada [155219-17 to J.-P.P.]; The RNA group is supported by a grant from the Universite de Sherbrooke; J.-P.P. holds the Research Chair of the Universite de Sherbrooke in RNA Structure and Genomics, and is a member of the Centre de Recherche du CHUS; B.B.-P. was a recipient of a VALi+d postdoctoral contract of the Generalitat Valenciana [APOSTD/2017/039]; Schleiff group is funded through the Deutsche Forschungsgemeinschaft [SFB 902]. Funding for open access charge: Spanish Ministry of Science, Innovation and Universities.Oxford University PressInstituto Universitario Mixto de Biología Molecular y Celular de PlantasDepartamento de BiotecnologíaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalMinisterio de Economía y CompetitividadGeneralitat ValencianaDeutsche ForschungsgemeinschaftNatural Sciences and Engineering Research Council of CanadaMinisterio de Ciencia e InnovaciónRepositorio Institucional de la Universitat Politècnica de València Riunet20192019-09-19journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/159849reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BIO2009-11818 Hipusinacion Del Factor Eif5A Y Muerte Celular Inducida Por Estres En PlantasNatural Sciences and Engineering Research Council of Canada NSERC 155219-17Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BIO2015-70483-R PAPEL DE LA ESPERMIDINA Y DE LA BIOSINTESIS DE PROTEINAS EN LA TOLERANCIA DEL POLEN A LAS ALTAS TEMPERATURASGeneralitat Valenciana https://doi.org/10.13039/501100003359 APOSTD%2F2017%2F039Deutsche Forschungsgemeinschaft https://doi.org/10.13039/501100001659 SFB 902Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BFU2009-11958 Señalizacion Y Respuesta Defensiva De Las Plantas Frente A PatogenosGeneralitat Valenciana https://doi.org/10.13039/501100003359 AICO%2F2017%2F048open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial (by-nc) http://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1598492026-06-13T07:49:27Z
score 15.300719

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