Cellular and molecular events that occur in the oocyte during prolonged ovarian storage in sheep

This article belongs to the Special Issue The Era of Assisted Reproductive Technologies Tailored to the Specific Necessities of Species, Industry and Case Reports.

Detalles Bibliográficos
Autores: Martín-Maestro, Alicia, Sánchez-Ajofrín, Irene, Maside, Carolina, Peris-Frau, Patricia, Medina-Chávez, Daniela-Alejandra, Cardoso, Beatriz, Navarro, José Carlos, Fernández-Santos, M. Rocío, Garde, José Julián, Soler, Ana J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/238692
Acceso en línea:http://hdl.handle.net/10261/238692
Access Level:acceso abierto
Palabra clave:Sheep
In vitro embryo production
Ovary storage
Oocyte
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spelling Cellular and molecular events that occur in the oocyte during prolonged ovarian storage in sheepMartín-Maestro, AliciaSánchez-Ajofrín, IreneMaside, CarolinaPeris-Frau, PatriciaMedina-Chávez, Daniela-AlejandraCardoso, BeatrizNavarro, José CarlosFernández-Santos, M. RocíoGarde, José JuliánSoler, Ana J.SheepIn vitro embryo productionOvary storageOocyteThis article belongs to the Special Issue The Era of Assisted Reproductive Technologies Tailored to the Specific Necessities of Species, Industry and Case Reports.For the past two decades, there has been a growing interest in the application of in vitro embryo production (IVP) in small ruminants such as sheep. To improve efficiency, a large number abattoir-derived ovaries must be used, and long distances from the laboratory are usually inevitable when adult animals are used. In that scenario, prolonged sheep ovary transportation may negatively affect oocyte developmental competence. Here, we evaluated the effect of ovary storage time (3, 5, 7, 9, 11 and 13 h) and the medium in which they were transported (TCM199 and saline solution) on oocyte quality. Thus, live/dead status, early apoptosis, DNA fragmentation, reduced glutathione (GSH) and reactive oxygen species (ROS) content, caspase-3 activity, mitochondrial membrane potential and distribution, and relative abundance of mRNA transcript levels were assessed in oocytes. After in vitro maturation (IVM), cumulus cell viability and quality, meiotic and fertilization competence, embryo rates and blastocyst quality were also evaluated. The results revealed that, after 7 h of storage, oocyte quality and developmental potential were significantly impaired since higher rates of dead oocytes and DNA fragmentation and lower rates of viable, matured and fertilized oocytes were observed. The percentage of cleavage, blastocyst rates and cumulus cell parameters (viability, active mitochondria and GSH/ROS ratio) were also decreased. Moreover, the preservation of ovaries in medium TCM199 had a detrimental effect on cumulus cells and oocyte competence. In conclusion, ovary transport times up to 5 h in saline solution are the most adequate storage conditions to maintain oocyte quality as well as developmental capacity in sheep. A strategy to rescue the poor developmental potential of stored oocytes will be necessary for successful production of high-quality embryos when longer ovarian preservation times are necessary.This research was funded by the Spanish Ministry of Economy and Competitiveness (AGL2017-89017-R). A.M.-M. was supported by a Ministry of Economy and Competitiveness scholarship. P.P.-F. and D.-A.M.-C. were supported by a University of Castilla-La Mancha scholarship. B.C. was supported by ERASMUS+−.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Universidad de Castilla La ManchaEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/238692reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-89017-RAGL2017-89017-R/AEI/10.13039/501100011033https://doi.org/10.3390/ani10122414Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2386922026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cellular and molecular events that occur in the oocyte during prolonged ovarian storage in sheep
title Cellular and molecular events that occur in the oocyte during prolonged ovarian storage in sheep
spellingShingle Cellular and molecular events that occur in the oocyte during prolonged ovarian storage in sheep
Martín-Maestro, Alicia
Sheep
In vitro embryo production
Ovary storage
Oocyte
title_short Cellular and molecular events that occur in the oocyte during prolonged ovarian storage in sheep
title_full Cellular and molecular events that occur in the oocyte during prolonged ovarian storage in sheep
title_fullStr Cellular and molecular events that occur in the oocyte during prolonged ovarian storage in sheep
title_full_unstemmed Cellular and molecular events that occur in the oocyte during prolonged ovarian storage in sheep
title_sort Cellular and molecular events that occur in the oocyte during prolonged ovarian storage in sheep
dc.creator.none.fl_str_mv Martín-Maestro, Alicia
Sánchez-Ajofrín, Irene
Maside, Carolina
Peris-Frau, Patricia
Medina-Chávez, Daniela-Alejandra
Cardoso, Beatriz
Navarro, José Carlos
Fernández-Santos, M. Rocío
Garde, José Julián
Soler, Ana J.
author Martín-Maestro, Alicia
author_facet Martín-Maestro, Alicia
Sánchez-Ajofrín, Irene
Maside, Carolina
Peris-Frau, Patricia
Medina-Chávez, Daniela-Alejandra
Cardoso, Beatriz
Navarro, José Carlos
Fernández-Santos, M. Rocío
Garde, José Julián
Soler, Ana J.
author_role author
author2 Sánchez-Ajofrín, Irene
Maside, Carolina
Peris-Frau, Patricia
Medina-Chávez, Daniela-Alejandra
Cardoso, Beatriz
Navarro, José Carlos
Fernández-Santos, M. Rocío
Garde, José Julián
Soler, Ana J.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Universidad de Castilla La Mancha
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Sheep
In vitro embryo production
Ovary storage
Oocyte
topic Sheep
In vitro embryo production
Ovary storage
Oocyte
description This article belongs to the Special Issue The Era of Assisted Reproductive Technologies Tailored to the Specific Necessities of Species, Industry and Case Reports.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/238692
url http://hdl.handle.net/10261/238692
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-89017-R
AGL2017-89017-R/AEI/10.13039/501100011033
https://doi.org/10.3390/ani10122414
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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