Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters

β-nerve growth factor (βNGF) plays a crucial role in reproductive physiology and sperm quality. Enzymatic activity of seminal plasma and vaginal fluids reduces available βNGF and it has been demonstrated that chitosan microspheres could protect rrβNGF from degradation. This study examined the effect...

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Detalles Bibliográficos
Autores: Gimeno Martos, Silvia, Bosa, Luigia, Lorenzo González, Pedro Luis, Arias Álvarez, María, Castellini, Cesare, García Rebollar, Pilar, García García, Rosa María
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/109346
Acceso en línea:https://hdl.handle.net/20.500.14352/109346
Access Level:acceso abierto
Palabra clave:636.082
Acrosome reaction
Capacitation
NGF
Rabbit sperm
Sperm motility
Producción animal
3104.11 Reproducción
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oai_identifier_str oai:docta.ucm.es:20.500.14352/109346
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repository_id_str
spelling Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parametersGimeno Martos, SilviaBosa, LuigiaLorenzo González, Pedro LuisArias Álvarez, MaríaCastellini, CesareGarcía Rebollar, PilarGarcía García, Rosa María636.082Acrosome reactionCapacitationNGFRabbit spermSperm motilityProducción animal3104.11 Reproducciónβ-nerve growth factor (βNGF) plays a crucial role in reproductive physiology and sperm quality. Enzymatic activity of seminal plasma and vaginal fluids reduces available βNGF and it has been demonstrated that chitosan microspheres could protect rrβNGF from degradation. This study examined the effects of microencapsulated rrbNGF with chitosan on rabbit sperm viability, motility and capacitation status. Results showed that 0.5 and 1 μg/mL of microencapsulated rrβNGF, as well as free rrβNGF or empty microspheres, did not adversely affect sperm viability or total motility after 2 h of incubation. However, the highest progressivity kinetic parameters were observed with 1 μg/mL free rrβNGF, while the highest curvilinear velocity (VCL) occurred with 0.5 μg/mL microencapsulated rrβNGF. Empty chitosan microspheres did not induce acrosome reaction (AR), but both concentrations of free and rrβNGFch favoured AR during in vitro incubation. The study suggests that using chitosan spheres did not show any adverse effects on sperm traits, unlike free rβNGF and rrβNGFch promoted capacitation and AR. Further research is needed to explore the potential of rrβNGFch in modifying in vitro capacitation and inducing ovulation during artificial insemination.WileyUniversidad Complutense de Madrid20242024-01-0120242024-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/109346reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-094404-B-C22 FUNCIONES FISIOLOGICAS DEL BETA NGF RECOMBINANTE DE CONEJO SOBRE LOS OOCITOS Y EMBRIONES IN VIVO E IN VITROopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1093462026-06-02T12:44:21Z
dc.title.none.fl_str_mv Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters
title Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters
spellingShingle Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters
Gimeno Martos, Silvia
636.082
Acrosome reaction
Capacitation
NGF
Rabbit sperm
Sperm motility
Producción animal
3104.11 Reproducción
title_short Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters
title_full Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters
title_fullStr Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters
title_full_unstemmed Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters
title_sort Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters
dc.creator.none.fl_str_mv Gimeno Martos, Silvia
Bosa, Luigia
Lorenzo González, Pedro Luis
Arias Álvarez, María
Castellini, Cesare
García Rebollar, Pilar
García García, Rosa María
author Gimeno Martos, Silvia
author_facet Gimeno Martos, Silvia
Bosa, Luigia
Lorenzo González, Pedro Luis
Arias Álvarez, María
Castellini, Cesare
García Rebollar, Pilar
García García, Rosa María
author_role author
author2 Bosa, Luigia
Lorenzo González, Pedro Luis
Arias Álvarez, María
Castellini, Cesare
García Rebollar, Pilar
García García, Rosa María
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 636.082
Acrosome reaction
Capacitation
NGF
Rabbit sperm
Sperm motility
Producción animal
3104.11 Reproducción
topic 636.082
Acrosome reaction
Capacitation
NGF
Rabbit sperm
Sperm motility
Producción animal
3104.11 Reproducción
description β-nerve growth factor (βNGF) plays a crucial role in reproductive physiology and sperm quality. Enzymatic activity of seminal plasma and vaginal fluids reduces available βNGF and it has been demonstrated that chitosan microspheres could protect rrβNGF from degradation. This study examined the effects of microencapsulated rrbNGF with chitosan on rabbit sperm viability, motility and capacitation status. Results showed that 0.5 and 1 μg/mL of microencapsulated rrβNGF, as well as free rrβNGF or empty microspheres, did not adversely affect sperm viability or total motility after 2 h of incubation. However, the highest progressivity kinetic parameters were observed with 1 μg/mL free rrβNGF, while the highest curvilinear velocity (VCL) occurred with 0.5 μg/mL microencapsulated rrβNGF. Empty chitosan microspheres did not induce acrosome reaction (AR), but both concentrations of free and rrβNGFch favoured AR during in vitro incubation. The study suggests that using chitosan spheres did not show any adverse effects on sperm traits, unlike free rβNGF and rrβNGFch promoted capacitation and AR. Further research is needed to explore the potential of rrβNGFch in modifying in vitro capacitation and inducing ovulation during artificial insemination.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-01-01
2024
2024-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/109346
url https://hdl.handle.net/20.500.14352/109346
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-094404-B-C22 FUNCIONES FISIOLOGICAS DEL BETA NGF RECOMBINANTE DE CONEJO SOBRE LOS OOCITOS Y EMBRIONES IN VIVO E IN VITRO
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410601428582400
score 15,81155

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