Interactions of polystyrene nanoplastics with in vitro models of the human intestinal barrier

The universal presence of micro-nanoplastics (MNPLs) and its relative unknown effects on human health is a concern demanding reliable data to evaluate their safety. As ingestion is one of the main exposure routes for humans, we have assessed their hazard using two in vitro models that simulate the h...

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Autores: Domenech, Josefa|||0000-0003-1375-0604, Hernández Bonilla, Alba|||0000-0001-6938-1233, Rubio Lorente, Laura|||0000-0002-2088-3803, Marcos Dauder, Ricardo|||0000-0001-7891-357X, Cortes Crignola, Constanza|||0000-0001-7254-9607
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:326495
Acceso en línea:https://ddd.uab.cat/record/326495
https://dx.doi.org/urn:doi:10.1007/s00204-020-02805-3
Access Level:acceso abierto
Palabra clave:Caco-2/HT29/Raji-B cells
Intestinal barrier
Nanoplastics
Styrene nanoparticles
SDG 3 - Good Health and Well-being
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spelling Interactions of polystyrene nanoplastics with in vitro models of the human intestinal barrierDomenech, Josefa|||0000-0003-1375-0604Hernández Bonilla, Alba|||0000-0001-6938-1233Rubio Lorente, Laura|||0000-0002-2088-3803Marcos Dauder, Ricardo|||0000-0001-7891-357XCortes Crignola, Constanza|||0000-0001-7254-9607Caco-2/HT29/Raji-B cellsIntestinal barrierNanoplasticsStyrene nanoparticlesSDG 3 - Good Health and Well-beingThe universal presence of micro-nanoplastics (MNPLs) and its relative unknown effects on human health is a concern demanding reliable data to evaluate their safety. As ingestion is one of the main exposure routes for humans, we have assessed their hazard using two in vitro models that simulate the human intestinal barrier and its associated lymphoid system. Two different coculture models (differentiated Caco-2/HT29 intestinal cells and Caco-2/HT29 + Raji-B cells) were exposed to polystyrene nanoparticles (PSNPs) for 24 h. Endpoints such as viability, membrane integrity, NPS localization and translocation, ROS induction, and genotoxic damage were evaluated to have a comprehensive view of their potentially harmful effects. No significant cytotoxic effects were observed in any of the analyzed systems. In addition, no adverse effects were detected in the integrity or in the permeability of the barrier model. Nevertheless, confocal microscopy analysis showed that MNPLs were highly uptaken by both of the barrier model systems, and that translocation across the membrane occurred. Thus, MNPLs were detected into Raji-B cells, placed in the basolateral compartment of the insert. The internalization followed a dose-dependent pattern, as assessed by flow cytometry. Nonetheless, no genotoxic or oxidative DNA damage induction was detected in either case. Finally, no variations in the transcription of oxidative and stress genes could be detected in any of the in vitro barrier models. Our results show that MNPLs can enter and cross the epithelial barrier of the digestive system, as demonstrated when Raji-B cells were included in the model, but without exerting apparent hazardous effects. 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/326495https://dx.doi.org/urn:doi:10.1007/s00204-020-02805-3reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 SAF2015-63519-Ropen accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3264952026-06-06T12:50:31Z
dc.title.none.fl_str_mv Interactions of polystyrene nanoplastics with in vitro models of the human intestinal barrier
title Interactions of polystyrene nanoplastics with in vitro models of the human intestinal barrier
spellingShingle Interactions of polystyrene nanoplastics with in vitro models of the human intestinal barrier
Domenech, Josefa|||0000-0003-1375-0604
Caco-2/HT29/Raji-B cells
Intestinal barrier
Nanoplastics
Styrene nanoparticles
SDG 3 - Good Health and Well-being
title_short Interactions of polystyrene nanoplastics with in vitro models of the human intestinal barrier
title_full Interactions of polystyrene nanoplastics with in vitro models of the human intestinal barrier
title_fullStr Interactions of polystyrene nanoplastics with in vitro models of the human intestinal barrier
title_full_unstemmed Interactions of polystyrene nanoplastics with in vitro models of the human intestinal barrier
title_sort Interactions of polystyrene nanoplastics with in vitro models of the human intestinal barrier
dc.creator.none.fl_str_mv Domenech, Josefa|||0000-0003-1375-0604
Hernández Bonilla, Alba|||0000-0001-6938-1233
Rubio Lorente, Laura|||0000-0002-2088-3803
Marcos Dauder, Ricardo|||0000-0001-7891-357X
Cortes Crignola, Constanza|||0000-0001-7254-9607
author Domenech, Josefa|||0000-0003-1375-0604
author_facet Domenech, Josefa|||0000-0003-1375-0604
Hernández Bonilla, Alba|||0000-0001-6938-1233
Rubio Lorente, Laura|||0000-0002-2088-3803
Marcos Dauder, Ricardo|||0000-0001-7891-357X
Cortes Crignola, Constanza|||0000-0001-7254-9607
author_role author
author2 Hernández Bonilla, Alba|||0000-0001-6938-1233
Rubio Lorente, Laura|||0000-0002-2088-3803
Marcos Dauder, Ricardo|||0000-0001-7891-357X
Cortes Crignola, Constanza|||0000-0001-7254-9607
author2_role author
author
author
author
dc.subject.none.fl_str_mv Caco-2/HT29/Raji-B cells
Intestinal barrier
Nanoplastics
Styrene nanoparticles
SDG 3 - Good Health and Well-being
topic Caco-2/HT29/Raji-B cells
Intestinal barrier
Nanoplastics
Styrene nanoparticles
SDG 3 - Good Health and Well-being
description The universal presence of micro-nanoplastics (MNPLs) and its relative unknown effects on human health is a concern demanding reliable data to evaluate their safety. As ingestion is one of the main exposure routes for humans, we have assessed their hazard using two in vitro models that simulate the human intestinal barrier and its associated lymphoid system. Two different coculture models (differentiated Caco-2/HT29 intestinal cells and Caco-2/HT29 + Raji-B cells) were exposed to polystyrene nanoparticles (PSNPs) for 24 h. Endpoints such as viability, membrane integrity, NPS localization and translocation, ROS induction, and genotoxic damage were evaluated to have a comprehensive view of their potentially harmful effects. No significant cytotoxic effects were observed in any of the analyzed systems. In addition, no adverse effects were detected in the integrity or in the permeability of the barrier model. Nevertheless, confocal microscopy analysis showed that MNPLs were highly uptaken by both of the barrier model systems, and that translocation across the membrane occurred. Thus, MNPLs were detected into Raji-B cells, placed in the basolateral compartment of the insert. The internalization followed a dose-dependent pattern, as assessed by flow cytometry. Nonetheless, no genotoxic or oxidative DNA damage induction was detected in either case. Finally, no variations in the transcription of oxidative and stress genes could be detected in any of the in vitro barrier models. Our results show that MNPLs can enter and cross the epithelial barrier of the digestive system, as demonstrated when Raji-B cells were included in the model, but without exerting apparent hazardous effects.
publishDate 2020
dc.date.none.fl_str_mv 2
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/326495
https://dx.doi.org/urn:doi:10.1007/s00204-020-02805-3
url https://ddd.uab.cat/record/326495
https://dx.doi.org/urn:doi:10.1007/s00204-020-02805-3
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SAF2015-63519-R
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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