Identification of diverse defense mechanisms in rainbow trout red blood cells in response to halted replication of VHS virus [version 2; referees: 2 approved, 1 approved with reservations]

Nombela Díaz, Iván; Puente Marín, Sara; CHICO GRAS, VERONICA; Villena, Alberto; Carracedo, Begoña; Ciordia, Sergio; Mena, María Carmen; Mercado, Luis; Pérez, Luis; Coll Morales, Julio; Estepa Pérez, Amparo; Ortega-Villaizan Romo, María del Mar

Identification of diverse defense mechanisms in rainbow trout red blood cells in response to halted replication of VHS virus [version 2; referees: 2 approved, 1 approved with reservations]

Background: It has been described that fish nucleated red blood cells (RBCs) generate a wide variety of immune-related gene transcripts when viruses highly replicate inside them and are their main target cell. The immune response and mechanisms of fish RBCs against viruses targeting other cells or t...

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Detalhes bibliográficos
Autores:	Nombela Díaz, Iván, Puente Marín, Sara, CHICO GRAS, VERONICA, Villena, Alberto, Carracedo, Begoña, Ciordia, Sergio, Mena, María Carmen, Mercado, Luis, Pérez, Luis, Coll Morales, Julio, Estepa Pérez, Amparo, Ortega-Villaizan Romo, María del Mar
Tipo de documento:	artigo
Data de publicação:	2018
País:	España
Recursos:	Universidad Miguel Hernández de Elche
Repositório:	REDIUMH. Depósito Digital de la UMH
OAI Identifier:	oai:dspace.umh.es:11000/6990
Acesso em linha:	http://hdl.handle.net/11000/6990
Access Level:	Acceso aberto
Palavra-chave:	nucleated red blood cells rainbow trout VHSV rhabdovirus immune response antiviral 577 - Bioquímica. Biología molecular. Biofísica

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network_acronym_str	ES
network_name_str	España
repository_id_str
spelling	Identification of diverse defense mechanisms in rainbow trout red blood cells in response to halted replication of VHS virus [version 2; referees: 2 approved, 1 approved with reservations]Nombela Díaz, IvánPuente Marín, SaraCHICO GRAS, VERONICAVillena, AlbertoCarracedo, BegoñaCiordia, SergioMena, María CarmenMercado, LuisPérez, LuisColl Morales, JulioEstepa Pérez, AmparoOrtega-Villaizan Romo, María del Marnucleated red blood cellsrainbow troutVHSVrhabdovirusimmune responseantiviral577 - Bioquímica. Biología molecular. BiofísicaBackground: It has been described that fish nucleated red blood cells (RBCs) generate a wide variety of immune-related gene transcripts when viruses highly replicate inside them and are their main target cell. The immune response and mechanisms of fish RBCs against viruses targeting other cells or tissues has not yet been explored and is the objective of our study. Methods: Rainbow trout RBCs were obtained from peripheral blood, ficoll purified and exposed to Viral Haemorrhagic Septicaemia virus (VHSV). Immune response was evaluated by means of RT-qPCR, flow cytometry, immunofluorescence and isobaric tag for relative and absolute quantification (iTRAQ) protein profiling. Results: VHSV N gene transcripts incremented early postexposure and were drastically decreased after 6 hours postexposure (hpe). The expression of type I interferon ( ifn1) gene was significantly downregulated at early postexposure (3 hpe), together with a gradual downregulation of interferon-inducible mx and pkr genes until 72 hpe. Type I IFN protein was downregulated and interferon-inducible Mx protein was maintained at basal levels. Co-culture assays of RBCs, previously exposed to UV-inactivated VHSV, and TSS (stromal cell line from spleen) revealed IFN crosstalk between both cell types. On the other hand, anti-microbial peptide β-defensin 1 and neutrophil chemotactic factor interleukin 8 were slightly upregulated in VHSV-exposed RBCs. iTRAQ profiling revealed that VHSV exposure can induce a global protein downregulation in rainbow trout RBCs, mainly related to RNA stability and proteasome pathways. Antioxidant/antiviral response is also suggested to be involved in the response of rainbow trout RBCs to VHSV. Conclusions: A variety of mechanisms are proposed to be implicated in the antiviral response of rainbow trout RBCs against VHSV halted infection. Ongoing research is focused on understanding the mechanisms in detail.This work was supported by the European Research Council (ERC starting grant 2014 GA639249).Instituto de Biología Molecular y Celular2021202120182021info:eu-repo/semantics/articleapplication/pdf42application/pdfhttp://hdl.handle.net/11000/6990reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheIngléshttps://doi.org/ 10.12688/f1000research.12985.2info:eu-repo/semantics/openAccessoai:dspace.umh.es:11000/69902026-05-27T13:36:21Z
dc.title.none.fl_str_mv	Identification of diverse defense mechanisms in rainbow trout red blood cells in response to halted replication of VHS virus [version 2; referees: 2 approved, 1 approved with reservations]
title	Identification of diverse defense mechanisms in rainbow trout red blood cells in response to halted replication of VHS virus [version 2; referees: 2 approved, 1 approved with reservations]
spellingShingle	Identification of diverse defense mechanisms in rainbow trout red blood cells in response to halted replication of VHS virus [version 2; referees: 2 approved, 1 approved with reservations] Nombela Díaz, Iván nucleated red blood cells rainbow trout VHSV rhabdovirus immune response antiviral 577 - Bioquímica. Biología molecular. Biofísica
title_short	Identification of diverse defense mechanisms in rainbow trout red blood cells in response to halted replication of VHS virus [version 2; referees: 2 approved, 1 approved with reservations]
title_full	Identification of diverse defense mechanisms in rainbow trout red blood cells in response to halted replication of VHS virus [version 2; referees: 2 approved, 1 approved with reservations]
title_fullStr	Identification of diverse defense mechanisms in rainbow trout red blood cells in response to halted replication of VHS virus [version 2; referees: 2 approved, 1 approved with reservations]
title_full_unstemmed	Identification of diverse defense mechanisms in rainbow trout red blood cells in response to halted replication of VHS virus [version 2; referees: 2 approved, 1 approved with reservations]
title_sort	Identification of diverse defense mechanisms in rainbow trout red blood cells in response to halted replication of VHS virus [version 2; referees: 2 approved, 1 approved with reservations]
dc.creator.none.fl_str_mv	Nombela Díaz, Iván Puente Marín, Sara CHICO GRAS, VERONICA Villena, Alberto Carracedo, Begoña Ciordia, Sergio Mena, María Carmen Mercado, Luis Pérez, Luis Coll Morales, Julio Estepa Pérez, Amparo Ortega-Villaizan Romo, María del Mar
author	Nombela Díaz, Iván
author_facet	Nombela Díaz, Iván Puente Marín, Sara CHICO GRAS, VERONICA Villena, Alberto Carracedo, Begoña Ciordia, Sergio Mena, María Carmen Mercado, Luis Pérez, Luis Coll Morales, Julio Estepa Pérez, Amparo Ortega-Villaizan Romo, María del Mar
author_role	author
author2	Puente Marín, Sara CHICO GRAS, VERONICA Villena, Alberto Carracedo, Begoña Ciordia, Sergio Mena, María Carmen Mercado, Luis Pérez, Luis Coll Morales, Julio Estepa Pérez, Amparo Ortega-Villaizan Romo, María del Mar
author2_role	author author author author author author author author author author author
dc.contributor.none.fl_str_mv	Instituto de Biología Molecular y Celular
dc.subject.none.fl_str_mv	nucleated red blood cells rainbow trout VHSV rhabdovirus immune response antiviral 577 - Bioquímica. Biología molecular. Biofísica
topic	nucleated red blood cells rainbow trout VHSV rhabdovirus immune response antiviral 577 - Bioquímica. Biología molecular. Biofísica
description	Background: It has been described that fish nucleated red blood cells (RBCs) generate a wide variety of immune-related gene transcripts when viruses highly replicate inside them and are their main target cell. The immune response and mechanisms of fish RBCs against viruses targeting other cells or tissues has not yet been explored and is the objective of our study. Methods: Rainbow trout RBCs were obtained from peripheral blood, ficoll purified and exposed to Viral Haemorrhagic Septicaemia virus (VHSV). Immune response was evaluated by means of RT-qPCR, flow cytometry, immunofluorescence and isobaric tag for relative and absolute quantification (iTRAQ) protein profiling. Results: VHSV N gene transcripts incremented early postexposure and were drastically decreased after 6 hours postexposure (hpe). The expression of type I interferon ( ifn1) gene was significantly downregulated at early postexposure (3 hpe), together with a gradual downregulation of interferon-inducible mx and pkr genes until 72 hpe. Type I IFN protein was downregulated and interferon-inducible Mx protein was maintained at basal levels. Co-culture assays of RBCs, previously exposed to UV-inactivated VHSV, and TSS (stromal cell line from spleen) revealed IFN crosstalk between both cell types. On the other hand, anti-microbial peptide β-defensin 1 and neutrophil chemotactic factor interleukin 8 were slightly upregulated in VHSV-exposed RBCs. iTRAQ profiling revealed that VHSV exposure can induce a global protein downregulation in rainbow trout RBCs, mainly related to RNA stability and proteasome pathways. Antioxidant/antiviral response is also suggested to be involved in the response of rainbow trout RBCs to VHSV. Conclusions: A variety of mechanisms are proposed to be implicated in the antiviral response of rainbow trout RBCs against VHSV halted infection. Ongoing research is focused on understanding the mechanisms in detail.
publishDate	2018
dc.date.none.fl_str_mv	2018 2021 2021 2021
dc.type.none.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11000/6990
url	http://hdl.handle.net/11000/6990
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	https://doi.org/ 10.12688/f1000research.12985.2
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf 42 application/pdf
dc.source.none.fl_str_mv	reponame:REDIUMH. Depósito Digital de la UMH instname:Universidad Miguel Hernández de Elche
instname_str	Universidad Miguel Hernández de Elche
reponame_str	REDIUMH. Depósito Digital de la UMH
collection	REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15.300719

Identification of diverse defense mechanisms in rainbow trout red blood cells in response to halted replication of VHS virus [version 2; referees: 2 approved, 1 approved with reservations]

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