Tubulin homolog TubZ in a phage-encodedpartition system

21 p.-4 fig.-2 tab.

Detalles Bibliográficos
Autores: Oliva, María A., Martín-Galiano, Antonio J., Shakaguchi, Yoshihiko, Andreu, José Manuel
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/345392
Acceso en línea:http://hdl.handle.net/10261/345392
Access Level:acceso abierto
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spelling Tubulin homolog TubZ in a phage-encodedpartition systemA TubZ tubulin homolog in a phage partition systemOliva, María A.Martín-Galiano, Antonio J.Shakaguchi, YoshihikoAndreu, José Manuel21 p.-4 fig.-2 tab.Partition systems are responsible for the process whereby large and essential plasmids are accurately positioned to daughter cells during bacterial division. They are typically made of three components: a centromere-like DNA zone, an adaptor protein, and an assembling protein that is either a Walker-box ATPase (type I) or an actin-like ATPase (type II). A recently described type III segregation system has a tubulin/FtsZ-like protein, called TubZ, for plasmid movement. Here, we present the 2.3 Å structure and dynamic assembly of a TubZ tubulin homolog from a bacteriophage and unravel the Clostridium botulinum phage c-st type III partition system. Using biochemical and biophysical approaches, we prove that a gene upstream from tubZ encodes the partner TubR and localize the centromeric region (tubS), both of which are essential for anchoring phage DNA to the motile TubZ filaments. Finally, we describe a conserved fourth component, TubY, which modulates the TubZ-R-S complex interaction.This work was supported by grants from Plan Nacional de Investigación BFU2008-00013, BFU2011-23416, and Comunidad de Madrid S2010/BMD-2353 (to J.M.A.).Peer reviewedNational Academy of Sciences (U.S.)Oliva, María A. [0000-0002-2215-4639]Martín-Galiano, Antonio J.[0000-0002-6662-329X]Andreu, José Manuel [0000-0001-8064-6933]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Preprintinfo:eu-repo/semantics/submittedVersionhttp://hdl.handle.net/10261/345392reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICINN//BFU2008-00013info:eu-repo/grantAgreement/MICINN//BFU2011-23416S2010/BMD-2353https://doi.org/10.1073/pnas.1121546109Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3453922026-05-22T06:33:51Z
dc.title.none.fl_str_mv Tubulin homolog TubZ in a phage-encodedpartition system
A TubZ tubulin homolog in a phage partition system
title Tubulin homolog TubZ in a phage-encodedpartition system
spellingShingle Tubulin homolog TubZ in a phage-encodedpartition system
Oliva, María A.
title_short Tubulin homolog TubZ in a phage-encodedpartition system
title_full Tubulin homolog TubZ in a phage-encodedpartition system
title_fullStr Tubulin homolog TubZ in a phage-encodedpartition system
title_full_unstemmed Tubulin homolog TubZ in a phage-encodedpartition system
title_sort Tubulin homolog TubZ in a phage-encodedpartition system
dc.creator.none.fl_str_mv Oliva, María A.
Martín-Galiano, Antonio J.
Shakaguchi, Yoshihiko
Andreu, José Manuel
author Oliva, María A.
author_facet Oliva, María A.
Martín-Galiano, Antonio J.
Shakaguchi, Yoshihiko
Andreu, José Manuel
author_role author
author2 Martín-Galiano, Antonio J.
Shakaguchi, Yoshihiko
Andreu, José Manuel
author2_role author
author
author
dc.contributor.none.fl_str_mv Oliva, María A. [0000-0002-2215-4639]
Martín-Galiano, Antonio J.[0000-0002-6662-329X]
Andreu, José Manuel [0000-0001-8064-6933]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description 21 p.-4 fig.-2 tab.
publishDate 2012
dc.date.none.fl_str_mv 2012
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Preprint
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/345392
url http://hdl.handle.net/10261/345392
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MICINN//BFU2008-00013
info:eu-repo/grantAgreement/MICINN//BFU2011-23416
S2010/BMD-2353
https://doi.org/10.1073/pnas.1121546109
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv National Academy of Sciences (U.S.)
publisher.none.fl_str_mv National Academy of Sciences (U.S.)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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