An Auxin-Mediated Regulatory Framework for Wound-Induced Adventitious Root Formation in Tomato Shoot Explants

Adventitious roots (ARs) are produced from non-root tissues in response to different environmental signals, such as abiotic stresses, or after wounding, in a complex developmental process that requires hormonal crosstalk. Here, we characterized AR formation in young seedlings of Solanum lycopersicum...

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Detalles Bibliográficos
Autores: Alaguero Cordovilla, Aurora, Sánchez García, Ana Belén, Ibáñez, Sergio, Albacete, Alfonso, Cano, Antonio, Acosta, Manuel, Pérez Pérez, José Manuel
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Miguel Hernández de Elche
Repositorio:REDIUMH. Depósito Digital de la UMH
OAI Identifier:oai:dspace.umh.es:11000/35297
Acceso en línea:https://hdl.handle.net/11000/35297
Access Level:acceso abierto
Palabra clave:solanum lycopersicum
cell reprogramming
auxin biosynthesis
polar auxin transport
de novo root formation
auxin response
hormone regulation
mechanical damage
adventitious rooting
tissue regeneration
CDU::5 - Ciencias puras y naturales::57 - Biología
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spelling An Auxin-Mediated Regulatory Framework for Wound-Induced Adventitious Root Formation in Tomato Shoot ExplantsAlaguero Cordovilla, AuroraSánchez García, Ana BelénIbáñez, SergioAlbacete, AlfonsoCano, AntonioAcosta, ManuelPérez Pérez, José Manuelsolanum lycopersicumcell reprogrammingauxin biosynthesispolar auxin transportde novo root formationauxin responsehormone regulationmechanical damageadventitious rootingtissue regenerationCDU::5 - Ciencias puras y naturales::57 - BiologíaAdventitious roots (ARs) are produced from non-root tissues in response to different environmental signals, such as abiotic stresses, or after wounding, in a complex developmental process that requires hormonal crosstalk. Here, we characterized AR formation in young seedlings of Solanum lycopersicum cv.‘Micro-Tom’ after whole root excision by means of physiological, geneticand molecular approaches. We found that a regulated basipetal auxin transport from the shoot and local auxin biosynthesis triggered by wounding are both required for the re-establishment of internal auxin gradients within the vasculature. This promotes cell proliferation at the distal cambium near the wound in well-defined positions of the basal hypocotyl and during a narrow developmental window. In addition, a pre-established pattern of differential auxin responses along the apical-basal axis of the hypocotyl and an as of yet unknown cell-autonomous inhibitory pathway contribute to the temporal and spatial patterning of the newly formed ARs on isolated hypocotyl explants. Our work provides an experimental outline for the dissection of wound-induced AR formation in tomato, a species that is suitable for molecular identification of gene regulatory networks via forward and reverse genetics approaches.WileyDepartamentos de la UMH::Biología Aplicada202520252021info:eu-repo/semantics/articleapplication/pdf60application/pdfhttps://hdl.handle.net/11000/35297reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheIngléshttps://doi.org/10.1111/pce.14001info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:dspace.umh.es:11000/352972026-05-27T13:36:21Z
dc.title.none.fl_str_mv An Auxin-Mediated Regulatory Framework for Wound-Induced Adventitious Root Formation in Tomato Shoot Explants
title An Auxin-Mediated Regulatory Framework for Wound-Induced Adventitious Root Formation in Tomato Shoot Explants
spellingShingle An Auxin-Mediated Regulatory Framework for Wound-Induced Adventitious Root Formation in Tomato Shoot Explants
Alaguero Cordovilla, Aurora
solanum lycopersicum
cell reprogramming
auxin biosynthesis
polar auxin transport
de novo root formation
auxin response
hormone regulation
mechanical damage
adventitious rooting
tissue regeneration
CDU::5 - Ciencias puras y naturales::57 - Biología
title_short An Auxin-Mediated Regulatory Framework for Wound-Induced Adventitious Root Formation in Tomato Shoot Explants
title_full An Auxin-Mediated Regulatory Framework for Wound-Induced Adventitious Root Formation in Tomato Shoot Explants
title_fullStr An Auxin-Mediated Regulatory Framework for Wound-Induced Adventitious Root Formation in Tomato Shoot Explants
title_full_unstemmed An Auxin-Mediated Regulatory Framework for Wound-Induced Adventitious Root Formation in Tomato Shoot Explants
title_sort An Auxin-Mediated Regulatory Framework for Wound-Induced Adventitious Root Formation in Tomato Shoot Explants
dc.creator.none.fl_str_mv Alaguero Cordovilla, Aurora
Sánchez García, Ana Belén
Ibáñez, Sergio
Albacete, Alfonso
Cano, Antonio
Acosta, Manuel
Pérez Pérez, José Manuel
author Alaguero Cordovilla, Aurora
author_facet Alaguero Cordovilla, Aurora
Sánchez García, Ana Belén
Ibáñez, Sergio
Albacete, Alfonso
Cano, Antonio
Acosta, Manuel
Pérez Pérez, José Manuel
author_role author
author2 Sánchez García, Ana Belén
Ibáñez, Sergio
Albacete, Alfonso
Cano, Antonio
Acosta, Manuel
Pérez Pérez, José Manuel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamentos de la UMH::Biología Aplicada
dc.subject.none.fl_str_mv solanum lycopersicum
cell reprogramming
auxin biosynthesis
polar auxin transport
de novo root formation
auxin response
hormone regulation
mechanical damage
adventitious rooting
tissue regeneration
CDU::5 - Ciencias puras y naturales::57 - Biología
topic solanum lycopersicum
cell reprogramming
auxin biosynthesis
polar auxin transport
de novo root formation
auxin response
hormone regulation
mechanical damage
adventitious rooting
tissue regeneration
CDU::5 - Ciencias puras y naturales::57 - Biología
description Adventitious roots (ARs) are produced from non-root tissues in response to different environmental signals, such as abiotic stresses, or after wounding, in a complex developmental process that requires hormonal crosstalk. Here, we characterized AR formation in young seedlings of Solanum lycopersicum cv.‘Micro-Tom’ after whole root excision by means of physiological, geneticand molecular approaches. We found that a regulated basipetal auxin transport from the shoot and local auxin biosynthesis triggered by wounding are both required for the re-establishment of internal auxin gradients within the vasculature. This promotes cell proliferation at the distal cambium near the wound in well-defined positions of the basal hypocotyl and during a narrow developmental window. In addition, a pre-established pattern of differential auxin responses along the apical-basal axis of the hypocotyl and an as of yet unknown cell-autonomous inhibitory pathway contribute to the temporal and spatial patterning of the newly formed ARs on isolated hypocotyl explants. Our work provides an experimental outline for the dissection of wound-induced AR formation in tomato, a species that is suitable for molecular identification of gene regulatory networks via forward and reverse genetics approaches.
publishDate 2021
dc.date.none.fl_str_mv 2021
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/11000/35297
url https://hdl.handle.net/11000/35297
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1111/pce.14001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
60
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:REDIUMH. Depósito Digital de la UMH
instname:Universidad Miguel Hernández de Elche
instname_str Universidad Miguel Hernández de Elche
reponame_str REDIUMH. Depósito Digital de la UMH
collection REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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