Nitric oxide signalling in the root is required for MYB72-dependent systemic resistance induced by Trichoderma volatiles in Arabidopsis.

29 páginas, 6 figuras

Detalhes bibliográficos
Autores: Pescador Azofra, Leyre, Fernández López, Iván, Pozo Jiménez, María José, Romero-Puertas, María C., Pieterse, Corné M. J., Martínez Medina, Ainhoa
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/247138
Acesso em linha:http://hdl.handle.net/10261/247138
Access Level:acceso abierto
Palavra-chave:Arabidopsis
Defence priming
Induced systemic resistance (ISR)
Microbial volatile compounds (VCs)
MYB72
Nitric oxide (NO)
Trichoderma
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spelling Nitric oxide signalling in the root is required for MYB72-dependent systemic resistance induced by Trichoderma volatiles in Arabidopsis.Pescador Azofra, LeyreFernández López, IvánPozo Jiménez, María JoséRomero-Puertas, María C.Pieterse, Corné M. J.Martínez Medina, AinhoaArabidopsisDefence primingInduced systemic resistance (ISR)Microbial volatile compounds (VCs)MYB72Nitric oxide (NO)Trichoderma29 páginas, 6 figurasVolatile compounds (VCs) of Trichoderma fungi trigger an induced systemic resistance (ISR) in Arabidopsis that is effective against a broad spectrum of pathogens. The root-specific transcription factor MYB72 is an early regulator of ISR and also controls the activation of iron deficiency responses. Nitric oxide (NO) is involved in the regulation of MYB72-dependent iron deficiency responses in Arabidopsis roots, but the role of NO in the regulation of MYB72 and ISR by Trichoderma VCs remains unexplored. Using in vitro bioassays, Trichoderma VCs were applied to Arabidopsis seedlings. Plant perception of Trichoderma VCs triggered a burst of NO in Arabidopsis roots. By suppressing this burst using a NO scavenger, we show the involvement of NO in Trichoderma VCs-mediated regulation of MYB72. Using a NO scavenger and the Arabidopsis lines myb72 and nia1nia2 in in planta bioassays, we demonstrate that NO-signalling is required in the roots for the activation of Trichoderma VCs-mediated ISR against the leaf pathogen Botrytis cinerea. Analysis of the defence-related genes PR1 and PDF1.2 point to the involvement of root NO in priming leaves for enhanced defence. Our results support a key role of root NO-signalling in the regulation of MYB72 during the activation of ISR by Trichoderma VCs.This research was supported by the grants OTR04036 from Fundación Salamanca Ciudad de Cultura y Saberes and Ayuntamiento de Salamanca; P12BIO296 from Junta de Andalucía; RTI2018-094350-B-C31 and GC2018-098372-B-100 from the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund (MCIU/AEI/ERDF); and Marie Skłodowska-Curie Intra-European Fellowship FP7-PEOPLE-2011-IEF no. 301662 (to AMM). AMM and LP acknowledge the support of the German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig funded by the German Research Foundation (FZT 118). LP further acknowledges to the PhD International Mobility Programme from University of Granada.Peer reviewedOxford University PressMinisterio de Ciencia, Innovación y Universidades (España)Ayuntamiento de SalamancaMartínez Medina, Ainhoa [0000-0001-5008-9865]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/247138reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/GC2018-098372-B-100info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094350-B-C31https://doi.org/10.1093/jxb/erab294Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2471382026-05-22T06:33:51Z
dc.title.none.fl_str_mv Nitric oxide signalling in the root is required for MYB72-dependent systemic resistance induced by Trichoderma volatiles in Arabidopsis.
title Nitric oxide signalling in the root is required for MYB72-dependent systemic resistance induced by Trichoderma volatiles in Arabidopsis.
spellingShingle Nitric oxide signalling in the root is required for MYB72-dependent systemic resistance induced by Trichoderma volatiles in Arabidopsis.
Pescador Azofra, Leyre
Arabidopsis
Defence priming
Induced systemic resistance (ISR)
Microbial volatile compounds (VCs)
MYB72
Nitric oxide (NO)
Trichoderma
title_short Nitric oxide signalling in the root is required for MYB72-dependent systemic resistance induced by Trichoderma volatiles in Arabidopsis.
title_full Nitric oxide signalling in the root is required for MYB72-dependent systemic resistance induced by Trichoderma volatiles in Arabidopsis.
title_fullStr Nitric oxide signalling in the root is required for MYB72-dependent systemic resistance induced by Trichoderma volatiles in Arabidopsis.
title_full_unstemmed Nitric oxide signalling in the root is required for MYB72-dependent systemic resistance induced by Trichoderma volatiles in Arabidopsis.
title_sort Nitric oxide signalling in the root is required for MYB72-dependent systemic resistance induced by Trichoderma volatiles in Arabidopsis.
dc.creator.none.fl_str_mv Pescador Azofra, Leyre
Fernández López, Iván
Pozo Jiménez, María José
Romero-Puertas, María C.
Pieterse, Corné M. J.
Martínez Medina, Ainhoa
author Pescador Azofra, Leyre
author_facet Pescador Azofra, Leyre
Fernández López, Iván
Pozo Jiménez, María José
Romero-Puertas, María C.
Pieterse, Corné M. J.
Martínez Medina, Ainhoa
author_role author
author2 Fernández López, Iván
Pozo Jiménez, María José
Romero-Puertas, María C.
Pieterse, Corné M. J.
Martínez Medina, Ainhoa
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Ayuntamiento de Salamanca
Martínez Medina, Ainhoa [0000-0001-5008-9865]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Arabidopsis
Defence priming
Induced systemic resistance (ISR)
Microbial volatile compounds (VCs)
MYB72
Nitric oxide (NO)
Trichoderma
topic Arabidopsis
Defence priming
Induced systemic resistance (ISR)
Microbial volatile compounds (VCs)
MYB72
Nitric oxide (NO)
Trichoderma
description 29 páginas, 6 figuras
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/247138
url http://hdl.handle.net/10261/247138
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/GC2018-098372-B-100
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094350-B-C31
https://doi.org/10.1093/jxb/erab294
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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