De Novo Design of Integrin a5ß1 Modulating Proteins to Enhance Biomaterial Properties.

Integrin a5ß1 is crucial for cell attachment and migration in development and tissue regeneration, and a5ß1 binding proteins can have considerable utility in regenerative medicine and next-generation therapeutics. We use computational protein design to create de novo a5ß1-specific modulating minipro...

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Detalhes bibliográficos
Autores: Wang X, Guillem-Marti J, Kumar S, Lee DS, Cabrerizo-Aguado D, Werther R, Alamo KAE, Zhao YT, Nguyen A, Kopyeva I, Huang B, Li J, Hao Y, Li X, Brizuela-Velasco A, Murray A, Gerben S, Roy A, DeForest CA, Springer T, Ruohola-Baker H, Cooper JA, Campbell MG, Manero JM, Ginebra MP, Baker D
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Fundació Sant Joan de Déu
Repositorio:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
OAI Identifier:oai:fsjd.fundanetsuite.com:p28729
Acesso em linha:https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=28729
Access Level:acceso abierto
Palavra-chave:RGD
biomaterial
de novo protein design
hydrogel
integrin a5ß1
regenerative medicine
titanium
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spelling De Novo Design of Integrin a5ß1 Modulating Proteins to Enhance Biomaterial Properties.Wang XGuillem-Marti JKumar SLee DSCabrerizo-Aguado DWerther RAlamo KAEZhao YTNguyen AKopyeva IHuang BLi JHao YLi XBrizuela-Velasco AMurray AGerben SRoy ADeForest CASpringer TRuohola-Baker HCooper JACampbell MGManero JMGinebra MPBaker DRGDbiomaterialde novo protein designhydrogelintegrin a5ß1regenerative medicinetitaniumIntegrin a5ß1 is crucial for cell attachment and migration in development and tissue regeneration, and a5ß1 binding proteins can have considerable utility in regenerative medicine and next-generation therapeutics. We use computational protein design to create de novo a5ß1-specific modulating miniprotein binders, called NeoNectins, that bind to and stabilize the open state of a5ß1. When immobilized onto titanium surfaces and throughout 3D hydrogels, the NeoNectins outperform native fibronectin (FN) and RGD peptides in enhancing cell attachment and spreading, and NeoNectin-grafted titanium implants outperformed FN- and RGD-grafted implants in animal models in promoting tissue integration and bone growth. NeoNectins should be broadly applicable for tissue engineering and biomedicine.WILEY-V C H VERLAG GMBH2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=28729ADVANCED MATERIALSISSN: 09359648ISSNe: 15214095reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déuinstname:Fundació Sant Joan de DéuInglésinfo:eu-repo/semantics/openAccessoai:fsjd.fundanetsuite.com:p287292026-05-27T12:37:41Z
dc.title.none.fl_str_mv De Novo Design of Integrin a5ß1 Modulating Proteins to Enhance Biomaterial Properties.
title De Novo Design of Integrin a5ß1 Modulating Proteins to Enhance Biomaterial Properties.
spellingShingle De Novo Design of Integrin a5ß1 Modulating Proteins to Enhance Biomaterial Properties.
Wang X
RGD
biomaterial
de novo protein design
hydrogel
integrin a5ß1
regenerative medicine
titanium
title_short De Novo Design of Integrin a5ß1 Modulating Proteins to Enhance Biomaterial Properties.
title_full De Novo Design of Integrin a5ß1 Modulating Proteins to Enhance Biomaterial Properties.
title_fullStr De Novo Design of Integrin a5ß1 Modulating Proteins to Enhance Biomaterial Properties.
title_full_unstemmed De Novo Design of Integrin a5ß1 Modulating Proteins to Enhance Biomaterial Properties.
title_sort De Novo Design of Integrin a5ß1 Modulating Proteins to Enhance Biomaterial Properties.
dc.creator.none.fl_str_mv Wang X
Guillem-Marti J
Kumar S
Lee DS
Cabrerizo-Aguado D
Werther R
Alamo KAE
Zhao YT
Nguyen A
Kopyeva I
Huang B
Li J
Hao Y
Li X
Brizuela-Velasco A
Murray A
Gerben S
Roy A
DeForest CA
Springer T
Ruohola-Baker H
Cooper JA
Campbell MG
Manero JM
Ginebra MP
Baker D
author Wang X
author_facet Wang X
Guillem-Marti J
Kumar S
Lee DS
Cabrerizo-Aguado D
Werther R
Alamo KAE
Zhao YT
Nguyen A
Kopyeva I
Huang B
Li J
Hao Y
Li X
Brizuela-Velasco A
Murray A
Gerben S
Roy A
DeForest CA
Springer T
Ruohola-Baker H
Cooper JA
Campbell MG
Manero JM
Ginebra MP
Baker D
author_role author
author2 Guillem-Marti J
Kumar S
Lee DS
Cabrerizo-Aguado D
Werther R
Alamo KAE
Zhao YT
Nguyen A
Kopyeva I
Huang B
Li J
Hao Y
Li X
Brizuela-Velasco A
Murray A
Gerben S
Roy A
DeForest CA
Springer T
Ruohola-Baker H
Cooper JA
Campbell MG
Manero JM
Ginebra MP
Baker D
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv RGD
biomaterial
de novo protein design
hydrogel
integrin a5ß1
regenerative medicine
titanium
topic RGD
biomaterial
de novo protein design
hydrogel
integrin a5ß1
regenerative medicine
titanium
description Integrin a5ß1 is crucial for cell attachment and migration in development and tissue regeneration, and a5ß1 binding proteins can have considerable utility in regenerative medicine and next-generation therapeutics. We use computational protein design to create de novo a5ß1-specific modulating miniprotein binders, called NeoNectins, that bind to and stabilize the open state of a5ß1. When immobilized onto titanium surfaces and throughout 3D hydrogels, the NeoNectins outperform native fibronectin (FN) and RGD peptides in enhancing cell attachment and spreading, and NeoNectin-grafted titanium implants outperformed FN- and RGD-grafted implants in animal models in promoting tissue integration and bone growth. NeoNectins should be broadly applicable for tissue engineering and biomedicine.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=28729
url https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=28729
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv WILEY-V C H VERLAG GMBH
publisher.none.fl_str_mv WILEY-V C H VERLAG GMBH
dc.source.none.fl_str_mv ADVANCED MATERIALS
ISSN: 09359648
ISSNe: 15214095
reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
instname:Fundació Sant Joan de Déu
instname_str Fundació Sant Joan de Déu
reponame_str r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
collection r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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