Circulating Cell Biomarkers in Pulmonary Arterial Hypertension

Tura-Ceide, Olga|||0000-0003-4334-9790; Blanco, Isabel|||0000-0001-9452-3432; Garcia-Lucio, Jéssica; del Pozo, Roberto; García, Agustín Roberto; Ferrer, Elisabet|||0000-0002-0900-5436; Crespo García, Isabel; Rodríguez-Chiaradia, Diego A.|||0000-0002-8566-4011; Simeón-Aznar, Carmen Pilar|||0000-0003-3390-9029; López-Meseguer, Manuel|||0000-0003-2650-9238; Martín-Ontiyuelo, Clara; Peinado, Víctor I.; Barberà i Mir, Joan Albert|||0000-0003-1469-4990

Circulating Cell Biomarkers in Pulmonary Arterial Hypertension

Background: Endothelial dysfunction is central to PAH. In this study, we simultaneously analysed circulating levels of endothelial microvesicles (EMVs) and progenitor cells (PCs) in PAH and in controls, as biomarkers of pulmonary endothelial integrity and evaluated differences among PAH subtypes and...

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Autores:	Tura-Ceide, Olga\|\|\|0000-0003-4334-9790, Blanco, Isabel\|\|\|0000-0001-9452-3432, Garcia-Lucio, Jéssica, del Pozo, Roberto, García, Agustín Roberto, Ferrer, Elisabet\|\|\|0000-0002-0900-5436, Crespo García, Isabel, Rodríguez-Chiaradia, Diego A.\|\|\|0000-0002-8566-4011, Simeón-Aznar, Carmen Pilar\|\|\|0000-0003-3390-9029, López-Meseguer, Manuel\|\|\|0000-0003-2650-9238, Martín-Ontiyuelo, Clara, Peinado, Víctor I., Barberà i Mir, Joan Albert\|\|\|0000-0003-1469-4990
Tipo de recurso:	artículo
Fecha de publicación:	2021
País:	España
Institución:	Universitat Autònoma de Barcelona
Repositorio:	Dipòsit Digital de Documents de la UAB
Idioma:	inglés
OAI Identifier:	oai:ddd.uab.cat:294821
Acceso en línea:	https://ddd.uab.cat/record/294821 https://dx.doi.org/urn:doi:10.3390/cells10071688
Access Level:	acceso abierto
Palabra clave:	Pulmonary arterial hypertension Endothelial dysfunction Biomarkers Progenitor cells Endothelial extracellular vesicles PAH-specific treatment

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spelling	Circulating Cell Biomarkers in Pulmonary Arterial HypertensionRelationship with Clinical Heterogeneity and Therapeutic ResponseTura-Ceide, Olga\|\|\|0000-0003-4334-9790Blanco, Isabel\|\|\|0000-0001-9452-3432Garcia-Lucio, Jéssicadel Pozo, RobertoGarcía, Agustín RobertoFerrer, Elisabet\|\|\|0000-0002-0900-5436Crespo García, IsabelRodríguez-Chiaradia, Diego A.\|\|\|0000-0002-8566-4011Simeón-Aznar, Carmen Pilar\|\|\|0000-0003-3390-9029López-Meseguer, Manuel\|\|\|0000-0003-2650-9238Martín-Ontiyuelo, ClaraPeinado, Víctor I.Barberà i Mir, Joan Albert\|\|\|0000-0003-1469-4990Pulmonary arterial hypertensionEndothelial dysfunctionBiomarkersProgenitor cellsEndothelial extracellular vesiclesPAH-specific treatmentBackground: Endothelial dysfunction is central to PAH. In this study, we simultaneously analysed circulating levels of endothelial microvesicles (EMVs) and progenitor cells (PCs) in PAH and in controls, as biomarkers of pulmonary endothelial integrity and evaluated differences among PAH subtypes and as a response to treatment. Methods: Forty-seven controls and 144 patients with PAH (52 idiopathic, 9 heritable, 31 associated with systemic sclerosis, 15 associated with other connective tissue diseases, 20 associated with HIV and 17 associated with portal hypertension) were evaluated. Forty-four patients with scleroderma and 22 with HIV infection, but without PAH, were also studied. Circulating levels of EMVs, total (CD31 + CD42b -) and activated (CD31 + CD42b - CD62E +), as well as circulating PCs (CD34 + CD133 + CD45 low) were measured by flow cytometry and the EMVs/PCs ratio was computed. In treatment-naïve patients, measurements were repeated after 3 months of PAH therapy. Results: Patients with PAH showed higher numbers of EMVs and a lower percentage of PCs, compared with healthy controls. The EMV/PC ratio was increased in PAH patients, and in patients with SSc or HIV without PAH. After starting PAH therapy, individual changes in EMVs and PCs were variable, without significant differences being observed as a group. Conclusion: PAH patients present disturbed vascular homeostasis, reflected in changes in circulating EMV and PC levels, which are not restored with PAH targeted therapy. Combined measurement of circulating EMVs and PCs could be foreseen as a potential biomarker of endothelial dysfunction in PAH. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/294821https://dx.doi.org/urn:doi:10.3390/cells10071688reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI12/00510Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI15/00582Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI18/00960Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 CP17/00114open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2948212026-06-06T12:50:31Z
dc.title.none.fl_str_mv	Circulating Cell Biomarkers in Pulmonary Arterial Hypertension Relationship with Clinical Heterogeneity and Therapeutic Response
title	Circulating Cell Biomarkers in Pulmonary Arterial Hypertension
spellingShingle	Circulating Cell Biomarkers in Pulmonary Arterial Hypertension Tura-Ceide, Olga\|\|\|0000-0003-4334-9790 Pulmonary arterial hypertension Endothelial dysfunction Biomarkers Progenitor cells Endothelial extracellular vesicles PAH-specific treatment
title_short	Circulating Cell Biomarkers in Pulmonary Arterial Hypertension
title_full	Circulating Cell Biomarkers in Pulmonary Arterial Hypertension
title_fullStr	Circulating Cell Biomarkers in Pulmonary Arterial Hypertension
title_full_unstemmed	Circulating Cell Biomarkers in Pulmonary Arterial Hypertension
title_sort	Circulating Cell Biomarkers in Pulmonary Arterial Hypertension
dc.creator.none.fl_str_mv	Tura-Ceide, Olga\|\|\|0000-0003-4334-9790 Blanco, Isabel\|\|\|0000-0001-9452-3432 Garcia-Lucio, Jéssica del Pozo, Roberto García, Agustín Roberto Ferrer, Elisabet\|\|\|0000-0002-0900-5436 Crespo García, Isabel Rodríguez-Chiaradia, Diego A.\|\|\|0000-0002-8566-4011 Simeón-Aznar, Carmen Pilar\|\|\|0000-0003-3390-9029 López-Meseguer, Manuel\|\|\|0000-0003-2650-9238 Martín-Ontiyuelo, Clara Peinado, Víctor I. Barberà i Mir, Joan Albert\|\|\|0000-0003-1469-4990
author	Tura-Ceide, Olga\|\|\|0000-0003-4334-9790
author_facet	Tura-Ceide, Olga\|\|\|0000-0003-4334-9790 Blanco, Isabel\|\|\|0000-0001-9452-3432 Garcia-Lucio, Jéssica del Pozo, Roberto García, Agustín Roberto Ferrer, Elisabet\|\|\|0000-0002-0900-5436 Crespo García, Isabel Rodríguez-Chiaradia, Diego A.\|\|\|0000-0002-8566-4011 Simeón-Aznar, Carmen Pilar\|\|\|0000-0003-3390-9029 López-Meseguer, Manuel\|\|\|0000-0003-2650-9238 Martín-Ontiyuelo, Clara Peinado, Víctor I. Barberà i Mir, Joan Albert\|\|\|0000-0003-1469-4990
author_role	author
author2	Blanco, Isabel\|\|\|0000-0001-9452-3432 Garcia-Lucio, Jéssica del Pozo, Roberto García, Agustín Roberto Ferrer, Elisabet\|\|\|0000-0002-0900-5436 Crespo García, Isabel Rodríguez-Chiaradia, Diego A.\|\|\|0000-0002-8566-4011 Simeón-Aznar, Carmen Pilar\|\|\|0000-0003-3390-9029 López-Meseguer, Manuel\|\|\|0000-0003-2650-9238 Martín-Ontiyuelo, Clara Peinado, Víctor I. Barberà i Mir, Joan Albert\|\|\|0000-0003-1469-4990
author2_role	author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Pulmonary arterial hypertension Endothelial dysfunction Biomarkers Progenitor cells Endothelial extracellular vesicles PAH-specific treatment
topic	Pulmonary arterial hypertension Endothelial dysfunction Biomarkers Progenitor cells Endothelial extracellular vesicles PAH-specific treatment
description	Background: Endothelial dysfunction is central to PAH. In this study, we simultaneously analysed circulating levels of endothelial microvesicles (EMVs) and progenitor cells (PCs) in PAH and in controls, as biomarkers of pulmonary endothelial integrity and evaluated differences among PAH subtypes and as a response to treatment. Methods: Forty-seven controls and 144 patients with PAH (52 idiopathic, 9 heritable, 31 associated with systemic sclerosis, 15 associated with other connective tissue diseases, 20 associated with HIV and 17 associated with portal hypertension) were evaluated. Forty-four patients with scleroderma and 22 with HIV infection, but without PAH, were also studied. Circulating levels of EMVs, total (CD31 + CD42b -) and activated (CD31 + CD42b - CD62E +), as well as circulating PCs (CD34 + CD133 + CD45 low) were measured by flow cytometry and the EMVs/PCs ratio was computed. In treatment-naïve patients, measurements were repeated after 3 months of PAH therapy. Results: Patients with PAH showed higher numbers of EMVs and a lower percentage of PCs, compared with healthy controls. The EMV/PC ratio was increased in PAH patients, and in patients with SSc or HIV without PAH. After starting PAH therapy, individual changes in EMVs and PCs were variable, without significant differences being observed as a group. Conclusion: PAH patients present disturbed vascular homeostasis, reflected in changes in circulating EMV and PC levels, which are not restored with PAH targeted therapy. Combined measurement of circulating EMVs and PCs could be foreseen as a potential biomarker of endothelial dysfunction in PAH.
publishDate	2021
dc.date.none.fl_str_mv	2 2021-01-01 2021 2021-01-01
dc.type.none.fl_str_mv	Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://ddd.uab.cat/record/294821 https://dx.doi.org/urn:doi:10.3390/cells10071688
url	https://ddd.uab.cat/record/294821 https://dx.doi.org/urn:doi:10.3390/cells10071688
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI12/00510 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI15/00582 Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI18/00960 Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 CP17/00114
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona
instname_str	Universitat Autònoma de Barcelona
reponame_str	Dipòsit Digital de Documents de la UAB
collection	Dipòsit Digital de Documents de la UAB
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Circulating Cell Biomarkers in Pulmonary Arterial Hypertension

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