β-Lactam TRPM8 Antagonist RGM8-51 Displays Antinociceptive Activity in Different Animal Models

Transient receptor potential melastatin subtype 8 (TRPM8) is a cation channel extensively expressed in sensory neurons and implicated in different painful states. However, the effectiveness of TRPM8 modulators for pain relief is still a matter of discussion, since structurally diverse mod ulators le...

Descripción completa

Detalles Bibliográficos
Autores: Martín Escura, Cristina, Medina Peris, Alicia, Spear, Luke, de la Torre-Martinez, Roberto, Olivos-Oré, Luis A., Barahona, M. Victoria, González-Rodríguez, Sara, Fernandez-Ballester, Gregorio, Fernandez-Carvajal, Asia, Artalejo, Antonio R., Ferrer-Montiel, Antonio, GONZALEZ-MUÑIZ, ROSARIO
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Miguel Hernández de Elche
Repositorio:REDIUMH. Depósito Digital de la UMH
OAI Identifier:oai:dspace.umh.es:11000/34395
Acceso en línea:https://hdl.handle.net/11000/34395
Access Level:acceso abierto
Palabra clave:TRPM8 channels
antagonist
β–lactam
oxaliplatin-induced peripheral neuropathy
CCI chronic neuropathic
nociception
NTG-induced hyperesthesia
CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica
id ES_6f67bc91c2b755542dd68ed6624aeddc
oai_identifier_str oai:dspace.umh.es:11000/34395
network_acronym_str ES
network_name_str España
repository_id_str
spelling β-Lactam TRPM8 Antagonist RGM8-51 Displays Antinociceptive Activity in Different Animal ModelsMartín Escura, CristinaMedina Peris, AliciaSpear, Lukede la Torre-Martinez, RobertoOlivos-Oré, Luis A.Barahona, M. VictoriaGonzález-Rodríguez, SaraFernandez-Ballester, GregorioFernandez-Carvajal, AsiaArtalejo, Antonio R.Ferrer-Montiel, AntonioGONZALEZ-MUÑIZ, ROSARIOTRPM8 channelsantagonistβ–lactamoxaliplatin-induced peripheral neuropathyCCI chronic neuropathicnociceptionNTG-induced hyperesthesiaCDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. BiofísicaTransient receptor potential melastatin subtype 8 (TRPM8) is a cation channel extensively expressed in sensory neurons and implicated in different painful states. However, the effectiveness of TRPM8 modulators for pain relief is still a matter of discussion, since structurally diverse mod ulators lead to different results, depending on the animal pain model. In this work, we described the antinociceptive activity of a β–lactam derivative, RGM8-51, showing good TRPM8 antagonist activity, and selectivity against related thermoTRP channels and other pain-mediating receptors. In primary cultures of rat dorsal root ganglion (DRG) neurons, RGM8-51 potently reduced menthol evoked neuronal firing without affecting the major ion conductances responsible for action potential generation. This compound has in vivo antinociceptive activity in response to cold, in a mouse model of oxaliplatin-induced peripheral neuropathy. In addition, it reduces cold, mechanical and heat hypersensitivity in a rat model of neuropathic pain arising after chronic constriction of the sciatic nerve. Furthermore, RGM8-51 exhibits mechanical hypersensitivity-relieving activity, in a mouse model of NTG-induced hyperesthesia. Taken together, these preclinical results substantiate that this TRPM8 antagonist is a promising pharmacological tool to study TRPM8-related diseases.MDPIDepartamentos de la UMH::Bioquímica y Biología Molecular202520252022info:eu-repo/semantics/articleapplication/pdf24application/pdfhttps://hdl.handle.net/11000/34395reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheInglés23https://doi.org/10.3390/ijms23052692info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:dspace.umh.es:11000/343952026-05-27T13:36:21Z
dc.title.none.fl_str_mv β-Lactam TRPM8 Antagonist RGM8-51 Displays Antinociceptive Activity in Different Animal Models
title β-Lactam TRPM8 Antagonist RGM8-51 Displays Antinociceptive Activity in Different Animal Models
spellingShingle β-Lactam TRPM8 Antagonist RGM8-51 Displays Antinociceptive Activity in Different Animal Models
Martín Escura, Cristina
TRPM8 channels
antagonist
β–lactam
oxaliplatin-induced peripheral neuropathy
CCI chronic neuropathic
nociception
NTG-induced hyperesthesia
CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica
title_short β-Lactam TRPM8 Antagonist RGM8-51 Displays Antinociceptive Activity in Different Animal Models
title_full β-Lactam TRPM8 Antagonist RGM8-51 Displays Antinociceptive Activity in Different Animal Models
title_fullStr β-Lactam TRPM8 Antagonist RGM8-51 Displays Antinociceptive Activity in Different Animal Models
title_full_unstemmed β-Lactam TRPM8 Antagonist RGM8-51 Displays Antinociceptive Activity in Different Animal Models
title_sort β-Lactam TRPM8 Antagonist RGM8-51 Displays Antinociceptive Activity in Different Animal Models
dc.creator.none.fl_str_mv Martín Escura, Cristina
Medina Peris, Alicia
Spear, Luke
de la Torre-Martinez, Roberto
Olivos-Oré, Luis A.
Barahona, M. Victoria
González-Rodríguez, Sara
Fernandez-Ballester, Gregorio
Fernandez-Carvajal, Asia
Artalejo, Antonio R.
Ferrer-Montiel, Antonio
GONZALEZ-MUÑIZ, ROSARIO
author Martín Escura, Cristina
author_facet Martín Escura, Cristina
Medina Peris, Alicia
Spear, Luke
de la Torre-Martinez, Roberto
Olivos-Oré, Luis A.
Barahona, M. Victoria
González-Rodríguez, Sara
Fernandez-Ballester, Gregorio
Fernandez-Carvajal, Asia
Artalejo, Antonio R.
Ferrer-Montiel, Antonio
GONZALEZ-MUÑIZ, ROSARIO
author_role author
author2 Medina Peris, Alicia
Spear, Luke
de la Torre-Martinez, Roberto
Olivos-Oré, Luis A.
Barahona, M. Victoria
González-Rodríguez, Sara
Fernandez-Ballester, Gregorio
Fernandez-Carvajal, Asia
Artalejo, Antonio R.
Ferrer-Montiel, Antonio
GONZALEZ-MUÑIZ, ROSARIO
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamentos de la UMH::Bioquímica y Biología Molecular
dc.subject.none.fl_str_mv TRPM8 channels
antagonist
β–lactam
oxaliplatin-induced peripheral neuropathy
CCI chronic neuropathic
nociception
NTG-induced hyperesthesia
CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica
topic TRPM8 channels
antagonist
β–lactam
oxaliplatin-induced peripheral neuropathy
CCI chronic neuropathic
nociception
NTG-induced hyperesthesia
CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica
description Transient receptor potential melastatin subtype 8 (TRPM8) is a cation channel extensively expressed in sensory neurons and implicated in different painful states. However, the effectiveness of TRPM8 modulators for pain relief is still a matter of discussion, since structurally diverse mod ulators lead to different results, depending on the animal pain model. In this work, we described the antinociceptive activity of a β–lactam derivative, RGM8-51, showing good TRPM8 antagonist activity, and selectivity against related thermoTRP channels and other pain-mediating receptors. In primary cultures of rat dorsal root ganglion (DRG) neurons, RGM8-51 potently reduced menthol evoked neuronal firing without affecting the major ion conductances responsible for action potential generation. This compound has in vivo antinociceptive activity in response to cold, in a mouse model of oxaliplatin-induced peripheral neuropathy. In addition, it reduces cold, mechanical and heat hypersensitivity in a rat model of neuropathic pain arising after chronic constriction of the sciatic nerve. Furthermore, RGM8-51 exhibits mechanical hypersensitivity-relieving activity, in a mouse model of NTG-induced hyperesthesia. Taken together, these preclinical results substantiate that this TRPM8 antagonist is a promising pharmacological tool to study TRPM8-related diseases.
publishDate 2022
dc.date.none.fl_str_mv 2022
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/11000/34395
url https://hdl.handle.net/11000/34395
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv 23
https://doi.org/10.3390/ijms23052692
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
24
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:REDIUMH. Depósito Digital de la UMH
instname:Universidad Miguel Hernández de Elche
instname_str Universidad Miguel Hernández de Elche
reponame_str REDIUMH. Depósito Digital de la UMH
collection REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410493791207424
score 15,811543