Screening system of cannabis sativa extracts based on their mitochondrial safety profile using cytochrome c oxidase activity as a biomarker

The development of Cannabis sativa strains with high cannabidiol (CBD) and low tetrahydrocannabinol (THC) content is a growing field of research, both for medical and recreational use. However, the mechanisms behind clinical actions of cannabinoids are still under investigation, although there is gr...

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Autores: Noskova, Ekaterina, Fernández, Roberto, García, Javier, Ochoa, Eneko, Domínguez-Fernández, Celtia, Adell Calduch, Albert, Laso, Antonio, Andrés, María Fe, González-Coloma, Azucena, Astigarraga, Egoitz, Barreda-Gómez, Gabriel
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad de Cantabria (UC)
Repositorio:UCrea Repositorio Abierto de la Universidad de Cantabria
Idioma:inglés
OAI Identifier:oai:repositorio.unican.es:10902/29924
Acceso en línea:https://hdl.handle.net/10902/29924
Access Level:acceso abierto
Palabra clave:Cannabinoids
Cannabis sativa extracts
THC
CBD
Cytochrome c oxidase
Mitochondrial activity
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spelling Screening system of cannabis sativa extracts based on their mitochondrial safety profile using cytochrome c oxidase activity as a biomarkerNoskova, EkaterinaFernández, RobertoGarcía, JavierOchoa, EnekoDomínguez-Fernández, CeltiaAdell Calduch, AlbertLaso, AntonioAndrés, María FeGonzález-Coloma, AzucenaAstigarraga, EgoitzBarreda-Gómez, GabrielCannabinoidsCannabis sativa extractsTHCCBDCytochrome c oxidaseMitochondrial activityThe development of Cannabis sativa strains with high cannabidiol (CBD) and low tetrahydrocannabinol (THC) content is a growing field of research, both for medical and recreational use. However, the mechanisms behind clinical actions of cannabinoids are still under investigation, although there is growing evidence that mitochondria play an important role in many of them. Numerous studies have described that cannabinoids modulate mitochondrial activity both through activation of mitochondrial cannabinoid receptors and through direct action on other proteins such as mitochondrial complexes involved in cellular respiration. Thus, the aim of this study was to determine the actions of a panel of extracts, isolated from high-CBD varieties of Cannabis sativa, on the activity of the mitochondrial electron transport chain complex IV, cytochrome c oxidase (CCO), in order to select those with a safer profile. After demonstrating that Cannabis sativa strains could be identified by cannabinoids content, concentration-response curves were performed with a collection of extracts from strains with high-CBD and low-THC content using bovine CCO. The CCO rate was clearly modified by specific extracts of Cannabis sativa plants compared to others. Half maximal inhibitory concentrations (IC50) of extracts and the inhibitory effects evoked at 1 × 10-⁴ g/mL displayed a significant correlation with the THC. Therefore, the screening of extracts based on CCO activity provides a powerful and rapid methodology to identify those plants with higher mitochondrial toxicity or even mito-protective actions.Funding: This work has been financed by grant PID 2019-106222RB-C31/SRA, the Spanish Government (Ayudas para contratos para la formación de doctores en empresas (Doctorados Industriales) 2020, DIN 2020-011349), and Instituto de Salud Carlos III (Grant number PI 19/00170).MDPIUniversidad de Cantabria20232023-01-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articlehttps://hdl.handle.net/10902/29924International Journal of Molecular Sciences, 2023, 24, 1315reponame:UCrea Repositorio Abierto de la Universidad de Cantabriainstname:Universidad de Cantabria (UC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositorio.unican.es:10902/299242026-06-02T12:39:31Z
dc.title.none.fl_str_mv Screening system of cannabis sativa extracts based on their mitochondrial safety profile using cytochrome c oxidase activity as a biomarker
title Screening system of cannabis sativa extracts based on their mitochondrial safety profile using cytochrome c oxidase activity as a biomarker
spellingShingle Screening system of cannabis sativa extracts based on their mitochondrial safety profile using cytochrome c oxidase activity as a biomarker
Noskova, Ekaterina
Cannabinoids
Cannabis sativa extracts
THC
CBD
Cytochrome c oxidase
Mitochondrial activity
title_short Screening system of cannabis sativa extracts based on their mitochondrial safety profile using cytochrome c oxidase activity as a biomarker
title_full Screening system of cannabis sativa extracts based on their mitochondrial safety profile using cytochrome c oxidase activity as a biomarker
title_fullStr Screening system of cannabis sativa extracts based on their mitochondrial safety profile using cytochrome c oxidase activity as a biomarker
title_full_unstemmed Screening system of cannabis sativa extracts based on their mitochondrial safety profile using cytochrome c oxidase activity as a biomarker
title_sort Screening system of cannabis sativa extracts based on their mitochondrial safety profile using cytochrome c oxidase activity as a biomarker
dc.creator.none.fl_str_mv Noskova, Ekaterina
Fernández, Roberto
García, Javier
Ochoa, Eneko
Domínguez-Fernández, Celtia
Adell Calduch, Albert
Laso, Antonio
Andrés, María Fe
González-Coloma, Azucena
Astigarraga, Egoitz
Barreda-Gómez, Gabriel
author Noskova, Ekaterina
author_facet Noskova, Ekaterina
Fernández, Roberto
García, Javier
Ochoa, Eneko
Domínguez-Fernández, Celtia
Adell Calduch, Albert
Laso, Antonio
Andrés, María Fe
González-Coloma, Azucena
Astigarraga, Egoitz
Barreda-Gómez, Gabriel
author_role author
author2 Fernández, Roberto
García, Javier
Ochoa, Eneko
Domínguez-Fernández, Celtia
Adell Calduch, Albert
Laso, Antonio
Andrés, María Fe
González-Coloma, Azucena
Astigarraga, Egoitz
Barreda-Gómez, Gabriel
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad de Cantabria
dc.subject.none.fl_str_mv Cannabinoids
Cannabis sativa extracts
THC
CBD
Cytochrome c oxidase
Mitochondrial activity
topic Cannabinoids
Cannabis sativa extracts
THC
CBD
Cytochrome c oxidase
Mitochondrial activity
description The development of Cannabis sativa strains with high cannabidiol (CBD) and low tetrahydrocannabinol (THC) content is a growing field of research, both for medical and recreational use. However, the mechanisms behind clinical actions of cannabinoids are still under investigation, although there is growing evidence that mitochondria play an important role in many of them. Numerous studies have described that cannabinoids modulate mitochondrial activity both through activation of mitochondrial cannabinoid receptors and through direct action on other proteins such as mitochondrial complexes involved in cellular respiration. Thus, the aim of this study was to determine the actions of a panel of extracts, isolated from high-CBD varieties of Cannabis sativa, on the activity of the mitochondrial electron transport chain complex IV, cytochrome c oxidase (CCO), in order to select those with a safer profile. After demonstrating that Cannabis sativa strains could be identified by cannabinoids content, concentration-response curves were performed with a collection of extracts from strains with high-CBD and low-THC content using bovine CCO. The CCO rate was clearly modified by specific extracts of Cannabis sativa plants compared to others. Half maximal inhibitory concentrations (IC50) of extracts and the inhibitory effects evoked at 1 × 10-⁴ g/mL displayed a significant correlation with the THC. Therefore, the screening of extracts based on CCO activity provides a powerful and rapid methodology to identify those plants with higher mitochondrial toxicity or even mito-protective actions.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10902/29924
url https://hdl.handle.net/10902/29924
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv International Journal of Molecular Sciences, 2023, 24, 1315
reponame:UCrea Repositorio Abierto de la Universidad de Cantabria
instname:Universidad de Cantabria (UC)
instname_str Universidad de Cantabria (UC)
reponame_str UCrea Repositorio Abierto de la Universidad de Cantabria
collection UCrea Repositorio Abierto de la Universidad de Cantabria
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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