Drought stress causes a reduction in the biosynthesis of ascorbic acid in soybean plants

Drought provokes a number of physiological changes in plants including oxidative damage. Ascorbic acid (AsA), also known as vitamin C, is one of the most abundant water-soluble antioxidant compound present in plant tissues. However, little is known on the regulation of AsA biosynthesis under drought...

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Autores: Seminario Huárriz, Amaia, Song, Li, Zulet González, Amaia, Nguyen, Henry T., González García, Esther, Larrainzar Rodríguez, Estíbaliz
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2017
País:España
Recursos:Universidad Pública de Navarra
Repositório:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/30445
Acesso em linha:https://hdl.handle.net/2454/30445
Access Level:Acceso aberto
Palavra-chave:Ascorbic acid
Drought stress
Legumes
Smirnoff-Wheeler pathway
Soybean
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spelling Drought stress causes a reduction in the biosynthesis of ascorbic acid in soybean plantsSeminario Huárriz, AmaiaSong, LiZulet González, AmaiaNguyen, Henry T.González García, EstherLarrainzar Rodríguez, EstíbalizAscorbic acidDrought stressLegumesSmirnoff-Wheeler pathwaySoybeanDrought provokes a number of physiological changes in plants including oxidative damage. Ascorbic acid (AsA), also known as vitamin C, is one of the most abundant water-soluble antioxidant compound present in plant tissues. However, little is known on the regulation of AsA biosynthesis under drought stress conditions. In the current work we analyze the effects of water deficit on the biosynthesis of AsA by measuring its content, in vivo biosynthesis and the expression level of genes in the Smirnoff-Wheeler pathway in one of the major legume crop, soybean (Glycine max L. Merr). Since the pathway has not been described in legumes, we first searched for the putative orthologous genes in the soybean genome. We observed a significant genetic redundancy, with multiple genes encoding each step in the pathway. Based on RNA-seq analysis, expression of the complete pathway was detected not only in leaves but also in root tissue. Putative paralogous genes presented differential expression patterns in response to drought, suggesting the existence of functional specialization mechanisms. We found a correlation between the levels of AsA and GalLDH biosynthetic rates in leaves of drought-stressed soybean plants. However, the levels of GalLDH transcripts did not show significant differences under water deficit conditions. Among the other known regulators of the pathway, only the expression of VTC1 genes correlated with the observed decline in AsA in leaves.This work was supported by the Spanish Ministry of Economy and Competitiveness (AGL 2011–23738 to EG and a “Juan de la Cierva” JCI-2012-13175 post-doctoral contract to EL) the Fundación Caja Navarra project FCN2016-7442 to EG and the project 2016/PI013 LEGUMINOSAS from the Government of Navarra to EL. AS received a Ph.D. fellowship from the Public University of Navarra (1287/2011).Frontiers MediaCiencias del Medio NaturalNatura Ingurunearen ZientziakGobierno de Navarra / Nafarroako Gobernua, 2016/PI013Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 1287/20112017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/ziphttps://hdl.handle.net/2454/30445reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/MICINN//AGL2011-23738© 2017 Seminario, Song, Zulet, Nguyen, González and Larrainzar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.https://creativecommons.org/licenses/by/3.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/304452026-06-17T12:41:47Z
dc.title.none.fl_str_mv Drought stress causes a reduction in the biosynthesis of ascorbic acid in soybean plants
title Drought stress causes a reduction in the biosynthesis of ascorbic acid in soybean plants
spellingShingle Drought stress causes a reduction in the biosynthesis of ascorbic acid in soybean plants
Seminario Huárriz, Amaia
Ascorbic acid
Drought stress
Legumes
Smirnoff-Wheeler pathway
Soybean
title_short Drought stress causes a reduction in the biosynthesis of ascorbic acid in soybean plants
title_full Drought stress causes a reduction in the biosynthesis of ascorbic acid in soybean plants
title_fullStr Drought stress causes a reduction in the biosynthesis of ascorbic acid in soybean plants
title_full_unstemmed Drought stress causes a reduction in the biosynthesis of ascorbic acid in soybean plants
title_sort Drought stress causes a reduction in the biosynthesis of ascorbic acid in soybean plants
dc.creator.none.fl_str_mv Seminario Huárriz, Amaia
Song, Li
Zulet González, Amaia
Nguyen, Henry T.
González García, Esther
Larrainzar Rodríguez, Estíbaliz
author Seminario Huárriz, Amaia
author_facet Seminario Huárriz, Amaia
Song, Li
Zulet González, Amaia
Nguyen, Henry T.
González García, Esther
Larrainzar Rodríguez, Estíbaliz
author_role author
author2 Song, Li
Zulet González, Amaia
Nguyen, Henry T.
González García, Esther
Larrainzar Rodríguez, Estíbaliz
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ciencias del Medio Natural
Natura Ingurunearen Zientziak
Gobierno de Navarra / Nafarroako Gobernua, 2016/PI013
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 1287/2011
dc.subject.none.fl_str_mv Ascorbic acid
Drought stress
Legumes
Smirnoff-Wheeler pathway
Soybean
topic Ascorbic acid
Drought stress
Legumes
Smirnoff-Wheeler pathway
Soybean
description Drought provokes a number of physiological changes in plants including oxidative damage. Ascorbic acid (AsA), also known as vitamin C, is one of the most abundant water-soluble antioxidant compound present in plant tissues. However, little is known on the regulation of AsA biosynthesis under drought stress conditions. In the current work we analyze the effects of water deficit on the biosynthesis of AsA by measuring its content, in vivo biosynthesis and the expression level of genes in the Smirnoff-Wheeler pathway in one of the major legume crop, soybean (Glycine max L. Merr). Since the pathway has not been described in legumes, we first searched for the putative orthologous genes in the soybean genome. We observed a significant genetic redundancy, with multiple genes encoding each step in the pathway. Based on RNA-seq analysis, expression of the complete pathway was detected not only in leaves but also in root tissue. Putative paralogous genes presented differential expression patterns in response to drought, suggesting the existence of functional specialization mechanisms. We found a correlation between the levels of AsA and GalLDH biosynthetic rates in leaves of drought-stressed soybean plants. However, the levels of GalLDH transcripts did not show significant differences under water deficit conditions. Among the other known regulators of the pathway, only the expression of VTC1 genes correlated with the observed decline in AsA in leaves.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/30445
url https://hdl.handle.net/2454/30445
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN//AGL2011-23738
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/3.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/3.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/zip
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
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