Characterization and identification of extracellular vesicles-coupled miRNA profiles in seminal plasma of fertile and subfertile rabbit bucks

13 Pág.

Detalhes bibliográficos
Autores: Sakr, O. G., Gad, Ahmed, Cañón-Beltrán, Karina, Cajas, Yulia N., Prochazka, Radek, Rizos, Dimitrios, Rebollar, P. G.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/331522
Acesso em linha:http://hdl.handle.net/10261/331522
https://api.elsevier.com/content/abstract/scopus_id/85163320764
Access Level:acceso abierto
Palavra-chave:Extracellular vesicles
Male fertility
Rabbit
Seminal plasma
Seminal quality
miRNA
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dc.title.none.fl_str_mv Characterization and identification of extracellular vesicles-coupled miRNA profiles in seminal plasma of fertile and subfertile rabbit bucks
title Characterization and identification of extracellular vesicles-coupled miRNA profiles in seminal plasma of fertile and subfertile rabbit bucks
spellingShingle Characterization and identification of extracellular vesicles-coupled miRNA profiles in seminal plasma of fertile and subfertile rabbit bucks
Sakr, O. G.
Extracellular vesicles
Male fertility
Rabbit
Seminal plasma
Seminal quality
miRNA
title_short Characterization and identification of extracellular vesicles-coupled miRNA profiles in seminal plasma of fertile and subfertile rabbit bucks
title_full Characterization and identification of extracellular vesicles-coupled miRNA profiles in seminal plasma of fertile and subfertile rabbit bucks
title_fullStr Characterization and identification of extracellular vesicles-coupled miRNA profiles in seminal plasma of fertile and subfertile rabbit bucks
title_full_unstemmed Characterization and identification of extracellular vesicles-coupled miRNA profiles in seminal plasma of fertile and subfertile rabbit bucks
title_sort Characterization and identification of extracellular vesicles-coupled miRNA profiles in seminal plasma of fertile and subfertile rabbit bucks
dc.creator.none.fl_str_mv Sakr, O. G.
Gad, Ahmed
Cañón-Beltrán, Karina
Cajas, Yulia N.
Prochazka, Radek
Rizos, Dimitrios
Rebollar, P. G.
author Sakr, O. G.
author_facet Sakr, O. G.
Gad, Ahmed
Cañón-Beltrán, Karina
Cajas, Yulia N.
Prochazka, Radek
Rizos, Dimitrios
Rebollar, P. G.
author_role author
author2 Gad, Ahmed
Cañón-Beltrán, Karina
Cajas, Yulia N.
Prochazka, Radek
Rizos, Dimitrios
Rebollar, P. G.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Ministry of Higher Education & Scientific Research (Egypt)
Ministry of Education, Youth and Sports (Czech Republic)
European Commission
Gad, Ahmed [0000-0001-9741-2105]
Cañón-Beltrán, Karina [0000-0002-0279-0857]
Cajas, Yulia N. [0000-0001-9791-6733]
Rizos, Dimitrios [0000-0001-6813-3940]
Rebollar, P. G. [0000-0003-4418-0157]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Extracellular vesicles
Male fertility
Rabbit
Seminal plasma
Seminal quality
miRNA
topic Extracellular vesicles
Male fertility
Rabbit
Seminal plasma
Seminal quality
miRNA
description 13 Pág.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/331522
https://api.elsevier.com/content/abstract/scopus_id/85163320764
url http://hdl.handle.net/10261/331522
https://api.elsevier.com/content/abstract/scopus_id/85163320764
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094404-B-C21
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111641RB-I00
Departamento de Reproducción Animal​ 
https://doi.org/10.1016/j.theriogenology.2023.06.020
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
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instname_str Consejo Superior de Investigaciones Científicas (CSIC)
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spelling Characterization and identification of extracellular vesicles-coupled miRNA profiles in seminal plasma of fertile and subfertile rabbit bucksSakr, O. G.Gad, AhmedCañón-Beltrán, KarinaCajas, Yulia N.Prochazka, RadekRizos, DimitriosRebollar, P. G.Extracellular vesiclesMale fertilityRabbitSeminal plasmaSeminal qualitymiRNA13 Pág.Seminal plasma (SP) provides essential nutrients, transport, and protection to the spermatozoa during their journey through the male and female reproductive tracts. Extracellular vesicles (EVs) are one of the main components of the SP with several biomolecular cargoes, including miRNAs, that can influence spermatozoa functions and interact with the cells of the female reproductive tract. This study aimed to isolate, characterize, and identify the miRNA expression profiles in the SP-EVs isolated from fertile (F) and subfertile (S) rabbit bucks that could serve as fertility biomarkers. In this study, the methods to isolate and identify EVs including exosomes, from SP of 3 F and S bucks have been developed. Ultracentrifugation and size exclusion chromatography analysis were using to isolate EVs from SP of F and S males that were qualitative and quantitively characterised using transmission electron microscopy, nanoparticle tracking analysis and western blotting. In addition, total RNA, including miRNA, was isolated, sequenced and identified from SP-EVs samples. Different SP-EVs concentrations (8.53 × 1011 ± 1.04 × 1011 and 1.84 × 1012 ± 1.75 × 1011 particles/mL of SP; P = 0.008), with a similar average size (143.9 ± 11.9 and 115.5 ± 2.4 nm; P = 0.7422) in F and S males, respectively was observed. Particle size was not significantly correlated with any kinetic parameter. The concentration of SP-EVs was positively correlated with the percentage of abnormal forms (r = 0.94; P < 0.05) and with the percentage of immotile spermatozoa (r = 0.88; P < 0.05). Small-RNA-seq analysis identified a total of 267 and 244 expressed miRNAs in the F and S groups, respectively. Two miRNAs (let-7b-5p and let-7a-5p) were the top most abundant miRNAs in both groups. Differential expression analysis revealed that 9 miRNAs including miR-190b-5p, miR-193b-5p, let-7b-3p, and miR-378-3p, and another 9 miRNAs including miR-7a-5p, miR-33a-5p, miR-449a-5p, and miR-146a-5p were significantly up- and downregulated in the F compared to the S group, respectively. The SP from F and S rabbit males contains EVs with different miRNA cargo correlated with spermatogenesis, homeostasis, and infertility, which could be used as biomarkers for male fertility and potential therapies for assisted reproductive technologies.Ministry of Science and Innovation (RTI 2018-094404-B-C-21 to PGR, PID2019-111641RB-I00 to DR) and the Ministry of Higher Education and Scientific Research of Egypt. The Ministry of Education, Youth and Sports of the Czech Republic, Operational Program Research, Development and Education, the project “EXCELLENCE in molecular aspects of the early development of vertebrates” Grant number: CZ.02.1.01/0.0/0.0/15_003/0000460. K C–B by a Maria Zambrano contract from European Union – NextGenerationEU.Peer reviewedElsevierMinisterio de Ciencia e Innovación (España)Ministry of Higher Education & Scientific Research (Egypt)Ministry of Education, Youth and Sports (Czech Republic)European CommissionGad, Ahmed [0000-0001-9741-2105]Cañón-Beltrán, Karina [0000-0002-0279-0857]Cajas, Yulia N. [0000-0001-9791-6733]Rizos, Dimitrios [0000-0001-6813-3940]Rebollar, P. G. [0000-0003-4418-0157]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/331522https://api.elsevier.com/content/abstract/scopus_id/85163320764reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094404-B-C21info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111641RB-I00Departamento de Reproducción Animal​ https://doi.org/10.1016/j.theriogenology.2023.06.020Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3315222026-05-22T06:33:51Z
score 15.812429

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