Morphometric signatures of exposure to endocrine disrupting chemicals in zebrafish eleutheroembryos

Understanding the mode of action of the different pollutants in human and wildlife health is a key step in environmental risk assessment. The aim of this study was to determine signatures that could link morphological phenotypes to the toxicity mechanisms of four Endocrine Disrupting Chemicals (EDCs...

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Detalles Bibliográficos
Autores: Martínez, Rubén, Herrero-Nogareda, Laia, Van Antro, Morgane, Campos, María Pilar, Casado, Marta, Barata Martí, Carlos, Piña, Benjamín, Navarro-Martín, Laia
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/187291
Acceso en línea:http://hdl.handle.net/10261/187291
Access Level:acceso abierto
Palabra clave:EDCs
Dose-response
Morphology
Endocrine disruption
Zebrafish
Eleutheroembryo
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spelling Morphometric signatures of exposure to endocrine disrupting chemicals in zebrafish eleutheroembryosMartínez, RubénHerrero-Nogareda, LaiaVan Antro, MorganeCampos, María PilarCasado, MartaBarata Martí, CarlosPiña, BenjamínNavarro-Martín, LaiaEDCsDose-responseMorphologyEndocrine disruptionZebrafishEleutheroembryoUnderstanding the mode of action of the different pollutants in human and wildlife health is a key step in environmental risk assessment. The aim of this study was to determine signatures that could link morphological phenotypes to the toxicity mechanisms of four Endocrine Disrupting Chemicals (EDCs): bisphenol A (BPA), perfluorooctanesulfonate potassium salt (PFOS), tributyltin chloride (TBT), and 17-ß-estradiol (E2). Zebrafish (Danio rerio) eleutheroembryos were exposed from 2 to 5 dpf to a wide range of BPA, PFOS, TBT and E2 concentrations. At the end of the exposures several morphometric features were assessed. Common and non-specific effects on larvae pigmentation or swim bladder area were observed after exposures to all compounds. BPA specifically induced yolk sac malabsorption syndrome and altered craniofacial parameters, whereas PFOS had specific effects on the notochord formation presenting higher rates of scoliosis and kyphosis. The main effect of E2 was an increase in the body length of the exposed eleutheroembryos. In the case of TBT, main alterations on the morphological traits were related to developmental delays. When integrating all morphometrical parameters, BPA showed the highest rates of malformations in terms of equilethality, followed by PFOS and, distantly, by TBT and E2. In the case of BPA and PFOS, we were able to relate our results with effects on the transcriptome and metabolome, previously reported. We propose that methodized morphometric analyses in zebrafish embryo model can be used as an inexpensive and easy screening tool to predict modes of action of a wide-range number of contaminants.This work was supported by a grant from the Spanish Ministry of Economy and Competitiveness (ref. CTQ2014-56777-R). LNM was supported by a H2020-Marie Skłodowska-Curie Action MSCA-IF-RI-2017 awarded by the European Commission (ref. 797725-EpiSTOX). RM was supported by an FPU predoctoral fellow from the Spanish Ministry of Education and Science (ref. FPU15/03332). We thank Chiara Luccarelli for helping with eleutheroembryos measurements.Peer reviewedElsevierMinisterio de Economía y Competitividad (España)European CommissionBarata Marti, Carlos [0000-0002-3360-0729]PIÑA CAPO, BENJAMIN CAYETANO [0000-0001-9216-2768]Navarro-Martín, Laia [0000-0001-6554-8833]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/187291reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/797725info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-56777-Rhttps://doi.org/10.1016/j.aquatox.2019.105232Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1872912026-05-22T06:33:51Z
dc.title.none.fl_str_mv Morphometric signatures of exposure to endocrine disrupting chemicals in zebrafish eleutheroembryos
title Morphometric signatures of exposure to endocrine disrupting chemicals in zebrafish eleutheroembryos
spellingShingle Morphometric signatures of exposure to endocrine disrupting chemicals in zebrafish eleutheroembryos
Martínez, Rubén
EDCs
Dose-response
Morphology
Endocrine disruption
Zebrafish
Eleutheroembryo
title_short Morphometric signatures of exposure to endocrine disrupting chemicals in zebrafish eleutheroembryos
title_full Morphometric signatures of exposure to endocrine disrupting chemicals in zebrafish eleutheroembryos
title_fullStr Morphometric signatures of exposure to endocrine disrupting chemicals in zebrafish eleutheroembryos
title_full_unstemmed Morphometric signatures of exposure to endocrine disrupting chemicals in zebrafish eleutheroembryos
title_sort Morphometric signatures of exposure to endocrine disrupting chemicals in zebrafish eleutheroembryos
dc.creator.none.fl_str_mv Martínez, Rubén
Herrero-Nogareda, Laia
Van Antro, Morgane
Campos, María Pilar
Casado, Marta
Barata Martí, Carlos
Piña, Benjamín
Navarro-Martín, Laia
author Martínez, Rubén
author_facet Martínez, Rubén
Herrero-Nogareda, Laia
Van Antro, Morgane
Campos, María Pilar
Casado, Marta
Barata Martí, Carlos
Piña, Benjamín
Navarro-Martín, Laia
author_role author
author2 Herrero-Nogareda, Laia
Van Antro, Morgane
Campos, María Pilar
Casado, Marta
Barata Martí, Carlos
Piña, Benjamín
Navarro-Martín, Laia
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Barata Marti, Carlos [0000-0002-3360-0729]
PIÑA CAPO, BENJAMIN CAYETANO [0000-0001-9216-2768]
Navarro-Martín, Laia [0000-0001-6554-8833]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv EDCs
Dose-response
Morphology
Endocrine disruption
Zebrafish
Eleutheroembryo
topic EDCs
Dose-response
Morphology
Endocrine disruption
Zebrafish
Eleutheroembryo
description Understanding the mode of action of the different pollutants in human and wildlife health is a key step in environmental risk assessment. The aim of this study was to determine signatures that could link morphological phenotypes to the toxicity mechanisms of four Endocrine Disrupting Chemicals (EDCs): bisphenol A (BPA), perfluorooctanesulfonate potassium salt (PFOS), tributyltin chloride (TBT), and 17-ß-estradiol (E2). Zebrafish (Danio rerio) eleutheroembryos were exposed from 2 to 5 dpf to a wide range of BPA, PFOS, TBT and E2 concentrations. At the end of the exposures several morphometric features were assessed. Common and non-specific effects on larvae pigmentation or swim bladder area were observed after exposures to all compounds. BPA specifically induced yolk sac malabsorption syndrome and altered craniofacial parameters, whereas PFOS had specific effects on the notochord formation presenting higher rates of scoliosis and kyphosis. The main effect of E2 was an increase in the body length of the exposed eleutheroembryos. In the case of TBT, main alterations on the morphological traits were related to developmental delays. When integrating all morphometrical parameters, BPA showed the highest rates of malformations in terms of equilethality, followed by PFOS and, distantly, by TBT and E2. In the case of BPA and PFOS, we were able to relate our results with effects on the transcriptome and metabolome, previously reported. We propose that methodized morphometric analyses in zebrafish embryo model can be used as an inexpensive and easy screening tool to predict modes of action of a wide-range number of contaminants.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/187291
url http://hdl.handle.net/10261/187291
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/797725
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-56777-R
https://doi.org/10.1016/j.aquatox.2019.105232
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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