Dataset collection of proteomic data of the paper "Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment"

Sánchez-López, L.; Chico, B.; García-Alonso, M. C.; Lozano, R.M.

Dataset collection of proteomic data of the paper "Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment"

In this work, a sequential covalent co-immobilization of graphene oxide (GO) and hyaluronic acid (HA) is performed to obtain a biocompatible wear-resistant nanocoating on the surface of the biomedical grade Cobalt-Chrome (CoCr) alloy. Nanocoated CoCr surfaces were characterized by Raman spectroscopy...

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Detalles Bibliográficos
Autores:	Sánchez-López, L., Chico, B., García-Alonso, M. C., Lozano, R.M.
Tipo de recurso:	conjunto de datos
Estado:	Versión enviada para evaluación y publicación
Fecha de publicación:	2024
País:	España
Institución:	Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:	DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:	oai:digital.csic.es:10261/356967
Acceso en línea:	http://hdl.handle.net/10261/356967
Access Level:	acceso abierto
Palabra clave:	CoCr Proteomic analysis wear-corrosion macrophages graphene oxide hyaluronic acid tribocorrosion covalent immobilization http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages

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network_name_str	España
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spelling	Dataset collection of proteomic data of the paper "Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment"Sánchez-López, L.Chico, B.García-Alonso, M. C.Lozano, R.M.CoCrProteomic analysiswear-corrosionmacrophagesgraphene oxidehyaluronic acidtribocorrosioncovalent immobilizationhttp://metadata.un.org/sdg/3Ensure healthy lives and promote well-being for all at all agesIn this work, a sequential covalent co-immobilization of graphene oxide (GO) and hyaluronic acid (HA) is performed to obtain a biocompatible wear-resistant nanocoating on the surface of the biomedical grade Cobalt-Chrome (CoCr) alloy. Nanocoated CoCr surfaces were characterized by Raman spectroscopy and electrochemical impedance spectroscopy (EIS) in 3 g/L HA electrolyte. Tribocorrosion tests of the nanocoated CoCr surfaces were carried out in a pin on flat tribometer. The biological response of covalently HA/GO biofunctionalized CoCr surfaces with and without wear-corrosion processes was studied through the analysis of the proteome of macrophages. Raman spectra revealed characteristic bands of GO and HA on the functionalized CoCr surfaces. The electrochemical response by EIS showed a stable and protective behavior over 23 days in the simulated biological environment. HA/GO covalently immobilized on CoCr alloy is able to protect the surface and reduce the wear volume released under tribocorrosion tests. Unsupervised classification analysis of the macrophage proteome via Hierarchical clustering and Principal Component Analysis (PCA) revealed that the covalent functionalization on CoCr enhances the macrophage biocompatibility in vitro. On the other hand, disruption of the HA/GO nanocoating by tribocorrosion processes induced a macrophage proteome which was differently clustered and was distantly located in the PCA space. In addition, tribocorrosion induced an increase in the percentage of upregulated and downregulated proteins in the macrophage proteome, revealing that disruption of the covalent nanocoating impacts the macrophage proteome. Although macrophage inflammation induced by tribocorrosion of HA/GO/CoCr surfaces is observed, it is ameliorated by the covalently grafting of HA, which provides immunomodulation by eliciting downregulations in characteristic pro-inflammatory signaling involved in inflammation and aseptic loosening of CoCr joint arthroplasties. Covalent HA/GO nanocoating on CoCr provides potential applications for in vivo joint prostheses led a reduced metal-induced inflammation and degradation by wear-corrosion in vivo.Proteome Dataset: Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive EnvironmentPeer reviewedDIGITAL.CSICGarcía-Alonso, María CristinaLozano Puerto, Rosa MaríaMinisterio de Ciencia, Innovación y Universidades (España)López-Sánchez, Luna [0000-0002-5065-9239]Chico, Belén [0000-0001-8697-6298]García-Alonso, María Cristina [0000-0003-0275-4626]Lozano, Rosa María [0000-0003-2762-6938]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1Preprintinfo:eu-repo/semantics/submittedVersionExcelhttp://hdl.handle.net/10261/356967reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#RTI2018-101506-B-C33RTI2018-101506-B-C31Journal of Biomedical Material Research. Part-A. (2024). DOI: 10.1002/jbm.a.37751ExcelSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3569672026-05-22T06:33:51Z
dc.title.none.fl_str_mv	Dataset collection of proteomic data of the paper "Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment"
title	Dataset collection of proteomic data of the paper "Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment"
spellingShingle	Dataset collection of proteomic data of the paper "Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment" Sánchez-López, L. CoCr Proteomic analysis wear-corrosion macrophages graphene oxide hyaluronic acid tribocorrosion covalent immobilization http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages
title_short	Dataset collection of proteomic data of the paper "Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment"
title_full	Dataset collection of proteomic data of the paper "Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment"
title_fullStr	Dataset collection of proteomic data of the paper "Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment"
title_full_unstemmed	Dataset collection of proteomic data of the paper "Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment"
title_sort	Dataset collection of proteomic data of the paper "Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment"
dc.creator.none.fl_str_mv	Sánchez-López, L. Chico, B. García-Alonso, M. C. Lozano, R.M.
author	Sánchez-López, L.
author_facet	Sánchez-López, L. Chico, B. García-Alonso, M. C. Lozano, R.M.
author_role	author
author2	Chico, B. García-Alonso, M. C. Lozano, R.M.
author2_role	author author author
dc.contributor.none.fl_str_mv	García-Alonso, María Cristina Lozano Puerto, Rosa María Ministerio de Ciencia, Innovación y Universidades (España) López-Sánchez, Luna [0000-0002-5065-9239] Chico, Belén [0000-0001-8697-6298] García-Alonso, María Cristina [0000-0003-0275-4626] Lozano, Rosa María [0000-0003-2762-6938] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv	CoCr Proteomic analysis wear-corrosion macrophages graphene oxide hyaluronic acid tribocorrosion covalent immobilization http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages
topic	CoCr Proteomic analysis wear-corrosion macrophages graphene oxide hyaluronic acid tribocorrosion covalent immobilization http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages
description	In this work, a sequential covalent co-immobilization of graphene oxide (GO) and hyaluronic acid (HA) is performed to obtain a biocompatible wear-resistant nanocoating on the surface of the biomedical grade Cobalt-Chrome (CoCr) alloy. Nanocoated CoCr surfaces were characterized by Raman spectroscopy and electrochemical impedance spectroscopy (EIS) in 3 g/L HA electrolyte. Tribocorrosion tests of the nanocoated CoCr surfaces were carried out in a pin on flat tribometer. The biological response of covalently HA/GO biofunctionalized CoCr surfaces with and without wear-corrosion processes was studied through the analysis of the proteome of macrophages. Raman spectra revealed characteristic bands of GO and HA on the functionalized CoCr surfaces. The electrochemical response by EIS showed a stable and protective behavior over 23 days in the simulated biological environment. HA/GO covalently immobilized on CoCr alloy is able to protect the surface and reduce the wear volume released under tribocorrosion tests. Unsupervised classification analysis of the macrophage proteome via Hierarchical clustering and Principal Component Analysis (PCA) revealed that the covalent functionalization on CoCr enhances the macrophage biocompatibility in vitro. On the other hand, disruption of the HA/GO nanocoating by tribocorrosion processes induced a macrophage proteome which was differently clustered and was distantly located in the PCA space. In addition, tribocorrosion induced an increase in the percentage of upregulated and downregulated proteins in the macrophage proteome, revealing that disruption of the covalent nanocoating impacts the macrophage proteome. Although macrophage inflammation induced by tribocorrosion of HA/GO/CoCr surfaces is observed, it is ameliorated by the covalently grafting of HA, which provides immunomodulation by eliciting downregulations in characteristic pro-inflammatory signaling involved in inflammation and aseptic loosening of CoCr joint arthroplasties. Covalent HA/GO nanocoating on CoCr provides potential applications for in vivo joint prostheses led a reduced metal-induced inflammation and degradation by wear-corrosion in vivo.
publishDate	2024
dc.date.none.fl_str_mv	2024 2024 2024
dc.type.none.fl_str_mv	info:eu-repo/semantics/dataset http://purl.org/coar/resource_type/c_ddb1 Preprint info:eu-repo/semantics/submittedVersion
format	dataset
status_str	submittedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/10261/356967
url	http://hdl.handle.net/10261/356967
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	#PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# RTI2018-101506-B-C33 RTI2018-101506-B-C31 Journal of Biomedical Material Research. Part-A. (2024). DOI: 10.1002/jbm.a.37751 Excel Sí
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	Excel
dc.publisher.none.fl_str_mv	DIGITAL.CSIC
publisher.none.fl_str_mv	DIGITAL.CSIC
dc.source.none.fl_str_mv	reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str	Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str	DIGITAL.CSIC. Repositorio Institucional del CSIC
collection	DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,811543

Dataset collection of proteomic data of the paper "Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment"

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