Production of cecropin A antimicrobial peptide in rice seed endosperm

Bundó Barberà, Mireia|||0000-0002-2765-1909; Montesinos Barreda, Laura|||0000-0003-4291-1227; Izquierdo, Esther; Campo, Sonia|||0000-0001-5477-3162; Mieulet, Delphine|||0000-0001-6220-0372; Guiderdoni, Emmanuel|||0000-0003-2760-2864; Rossignol, Michel; Badosa Romañó, Esther|||0000-0001-7776-8286; Montesinos Seguí, Emilio|||0000-0003-4455-8332; San Segundo, Blanca|||0000-0001-7409-3172; Coca López, María|||0000-0002-5578-3175

Production of cecropin A antimicrobial peptide in rice seed endosperm

Background: Cecropin A is a natural antimicrobial peptide that exhibits rapid, potent and long-lasting lytic activity against a broad spectrum of pathogens, thus having great biotechnological potential. Here, we report a system for producing bioactive cecropin A in rice seeds. - Results: transgenic...

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Detalles Bibliográficos
Autores:	Bundó Barberà, Mireia\|\|\|0000-0002-2765-1909, Montesinos Barreda, Laura\|\|\|0000-0003-4291-1227, Izquierdo, Esther, Campo, Sonia\|\|\|0000-0001-5477-3162, Mieulet, Delphine\|\|\|0000-0001-6220-0372, Guiderdoni, Emmanuel\|\|\|0000-0003-2760-2864, Rossignol, Michel, Badosa Romañó, Esther\|\|\|0000-0001-7776-8286, Montesinos Seguí, Emilio\|\|\|0000-0003-4455-8332, San Segundo, Blanca\|\|\|0000-0001-7409-3172, Coca López, María\|\|\|0000-0002-5578-3175
Tipo de recurso:	artículo
Fecha de publicación:	2014
País:	España
Institución:	Universitat Autònoma de Barcelona
Repositorio:	Dipòsit Digital de Documents de la UAB
Idioma:	inglés
OAI Identifier:	oai:ddd.uab.cat:175933
Acceso en línea:	https://ddd.uab.cat/record/175933 https://dx.doi.org/urn:doi:10.1186/1471-2229-14-102
Access Level:	acceso abierto
Palabra clave:	Rice Antimicrobial peptides Cecropin A Endosperm Protein bodies Pathogen resistance Fusarium verticillioides Dickeya dadantii Oryza sativa

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spelling	Production of cecropin A antimicrobial peptide in rice seed endospermBundó Barberà, Mireia\|\|\|0000-0002-2765-1909Montesinos Barreda, Laura\|\|\|0000-0003-4291-1227Izquierdo, EstherCampo, Sonia\|\|\|0000-0001-5477-3162Mieulet, Delphine\|\|\|0000-0001-6220-0372Guiderdoni, Emmanuel\|\|\|0000-0003-2760-2864Rossignol, MichelBadosa Romañó, Esther\|\|\|0000-0001-7776-8286Montesinos Seguí, Emilio\|\|\|0000-0003-4455-8332San Segundo, Blanca\|\|\|0000-0001-7409-3172Coca López, María\|\|\|0000-0002-5578-3175RiceAntimicrobial peptidesCecropin AEndospermProtein bodiesPathogen resistanceFusarium verticillioidesDickeya dadantiiOryza sativaBackground: Cecropin A is a natural antimicrobial peptide that exhibits rapid, potent and long-lasting lytic activity against a broad spectrum of pathogens, thus having great biotechnological potential. Here, we report a system for producing bioactive cecropin A in rice seeds. - Results: transgenic rice plants expressing a codon-optimized synthetic cecropin A gene drived by an endosperm-specific promoter, either the glutelin B1 or glutelin B4 promoter, were generated. The signal peptide sequence from either the glutelin B1 or the glutelin B4 were N-terminally fused to the coding sequence of the cecropin A. We also studied whether the presence of the KDEL endoplasmic reticulum retention signal at the C-terminal has an effect on cecropin A subcellular localization and accumulation. The transgenic rice plants showed stable transgene integration and inheritance. We show that cecropin A accumulates in protein storage bodies in the rice endosperm, particularly in type II protein bodies, supporting that the glutelin N-terminal signal peptides play a crucial role in directing the cecropin A to this organelle, independently of being tagged with the KDEL endoplasmic reticulum retention signal. The production of cecropin A in transgenic rice seeds did not affect seed viability or seedling growth. Furthermore, transgenic cecropin A seeds exhibited resistance to infection by fungal and bacterial pathogens (Fusarium verticillioides and Dickeya dadantii, respectively) indicating that the in planta-produced cecropin A is biologically active. - Conclusions: rice seeds can sustain bioactive cecropin A production and accumulation in protein bodies. The system might benefit the production of this antimicrobial agent for subsequent applications in crop protection and food preservation. 22014-01-0120142014-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/175933https://dx.doi.org/urn:doi:10.1186/1471-2229-14-102reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 EUI2008-03769Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 EUI2008-03572Ministerio de Educación y Ciencia https://doi.org/10.13039/501100004837 CSD2007-00036Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2008/SGR-812open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1759332026-06-06T12:50:31Z
dc.title.none.fl_str_mv	Production of cecropin A antimicrobial peptide in rice seed endosperm
title	Production of cecropin A antimicrobial peptide in rice seed endosperm
spellingShingle	Production of cecropin A antimicrobial peptide in rice seed endosperm Bundó Barberà, Mireia\|\|\|0000-0002-2765-1909 Rice Antimicrobial peptides Cecropin A Endosperm Protein bodies Pathogen resistance Fusarium verticillioides Dickeya dadantii Oryza sativa
title_short	Production of cecropin A antimicrobial peptide in rice seed endosperm
title_full	Production of cecropin A antimicrobial peptide in rice seed endosperm
title_fullStr	Production of cecropin A antimicrobial peptide in rice seed endosperm
title_full_unstemmed	Production of cecropin A antimicrobial peptide in rice seed endosperm
title_sort	Production of cecropin A antimicrobial peptide in rice seed endosperm
dc.creator.none.fl_str_mv	Bundó Barberà, Mireia\|\|\|0000-0002-2765-1909 Montesinos Barreda, Laura\|\|\|0000-0003-4291-1227 Izquierdo, Esther Campo, Sonia\|\|\|0000-0001-5477-3162 Mieulet, Delphine\|\|\|0000-0001-6220-0372 Guiderdoni, Emmanuel\|\|\|0000-0003-2760-2864 Rossignol, Michel Badosa Romañó, Esther\|\|\|0000-0001-7776-8286 Montesinos Seguí, Emilio\|\|\|0000-0003-4455-8332 San Segundo, Blanca\|\|\|0000-0001-7409-3172 Coca López, María\|\|\|0000-0002-5578-3175
author	Bundó Barberà, Mireia\|\|\|0000-0002-2765-1909
author_facet	Bundó Barberà, Mireia\|\|\|0000-0002-2765-1909 Montesinos Barreda, Laura\|\|\|0000-0003-4291-1227 Izquierdo, Esther Campo, Sonia\|\|\|0000-0001-5477-3162 Mieulet, Delphine\|\|\|0000-0001-6220-0372 Guiderdoni, Emmanuel\|\|\|0000-0003-2760-2864 Rossignol, Michel Badosa Romañó, Esther\|\|\|0000-0001-7776-8286 Montesinos Seguí, Emilio\|\|\|0000-0003-4455-8332 San Segundo, Blanca\|\|\|0000-0001-7409-3172 Coca López, María\|\|\|0000-0002-5578-3175
author_role	author
author2	Montesinos Barreda, Laura\|\|\|0000-0003-4291-1227 Izquierdo, Esther Campo, Sonia\|\|\|0000-0001-5477-3162 Mieulet, Delphine\|\|\|0000-0001-6220-0372 Guiderdoni, Emmanuel\|\|\|0000-0003-2760-2864 Rossignol, Michel Badosa Romañó, Esther\|\|\|0000-0001-7776-8286 Montesinos Seguí, Emilio\|\|\|0000-0003-4455-8332 San Segundo, Blanca\|\|\|0000-0001-7409-3172 Coca López, María\|\|\|0000-0002-5578-3175
author2_role	author author author author author author author author author author
dc.subject.none.fl_str_mv	Rice Antimicrobial peptides Cecropin A Endosperm Protein bodies Pathogen resistance Fusarium verticillioides Dickeya dadantii Oryza sativa
topic	Rice Antimicrobial peptides Cecropin A Endosperm Protein bodies Pathogen resistance Fusarium verticillioides Dickeya dadantii Oryza sativa
description	Background: Cecropin A is a natural antimicrobial peptide that exhibits rapid, potent and long-lasting lytic activity against a broad spectrum of pathogens, thus having great biotechnological potential. Here, we report a system for producing bioactive cecropin A in rice seeds. - Results: transgenic rice plants expressing a codon-optimized synthetic cecropin A gene drived by an endosperm-specific promoter, either the glutelin B1 or glutelin B4 promoter, were generated. The signal peptide sequence from either the glutelin B1 or the glutelin B4 were N-terminally fused to the coding sequence of the cecropin A. We also studied whether the presence of the KDEL endoplasmic reticulum retention signal at the C-terminal has an effect on cecropin A subcellular localization and accumulation. The transgenic rice plants showed stable transgene integration and inheritance. We show that cecropin A accumulates in protein storage bodies in the rice endosperm, particularly in type II protein bodies, supporting that the glutelin N-terminal signal peptides play a crucial role in directing the cecropin A to this organelle, independently of being tagged with the KDEL endoplasmic reticulum retention signal. The production of cecropin A in transgenic rice seeds did not affect seed viability or seedling growth. Furthermore, transgenic cecropin A seeds exhibited resistance to infection by fungal and bacterial pathogens (Fusarium verticillioides and Dickeya dadantii, respectively) indicating that the in planta-produced cecropin A is biologically active. - Conclusions: rice seeds can sustain bioactive cecropin A production and accumulation in protein bodies. The system might benefit the production of this antimicrobial agent for subsequent applications in crop protection and food preservation.
publishDate	2014
dc.date.none.fl_str_mv	2 2014-01-01 2014 2014-01-01
dc.type.none.fl_str_mv	Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://ddd.uab.cat/record/175933 https://dx.doi.org/urn:doi:10.1186/1471-2229-14-102
url	https://ddd.uab.cat/record/175933 https://dx.doi.org/urn:doi:10.1186/1471-2229-14-102
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 EUI2008-03769 Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 EUI2008-03572 Ministerio de Educación y Ciencia https://doi.org/10.13039/501100004837 CSD2007-00036 Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2008/SGR-812
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona
instname_str	Universitat Autònoma de Barcelona
reponame_str	Dipòsit Digital de Documents de la UAB
collection	Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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Production of cecropin A antimicrobial peptide in rice seed endosperm

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