Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing

Ratiometric fluorescent nanothermometers with near-infrared emission play an important role in in vivo sensing since they can be used as intracellular thermal sensing probes with high spatial resolution and high sensitivity, to investigate cellular functions of interest in diagnosis and therapy, whe...

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Autores: Blasi D, Gonzalez-Pato N, Rodriguez Rodriguez X, Diez-Zabala I, Srinivasan SY, Camarero N, Esquivias O, Roldán M, Guasch J, Laromaine A, Gorostiza P, Veciana J, Ratera I
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Fundació Sant Joan de Déu
Repositorio:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
OAI Identifier:oai:fsjd.fundanetsuite.com:p23426
Acceso en línea:https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=23426
Access Level:acceso abierto
Palabra clave:Caenorhabditis elegans
excimer emission
in vivo sensing
luminescence
organic radical nanoparticles
ratiometric nanothermometers
trityl radicals
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spelling Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo SensingBlasi DGonzalez-Pato NRodriguez Rodriguez XDiez-Zabala ISrinivasan SYCamarero NEsquivias ORoldán MGuasch JLaromaine AGorostiza PVeciana JRatera ICaenorhabditis elegansexcimer emissionin vivo sensingluminescenceorganic radical nanoparticlesratiometric nanothermometerstrityl radicalsRatiometric fluorescent nanothermometers with near-infrared emission play an important role in in vivo sensing since they can be used as intracellular thermal sensing probes with high spatial resolution and high sensitivity, to investigate cellular functions of interest in diagnosis and therapy, where current approaches are not effective. Herein, the temperature-dependent fluorescence of organic nanoparticles is designed, synthesized, and studied based on the dual emission, generated by monomer and excimer species, of the tris(2,4,6-trichlorophenyl)methyl radical (TTM) doping organic nanoparticles (TTMd-ONPs), made of optically neutral tris(2,4,6-trichlorophenyl)methane (TTM-aH), acting as a matrix. The excimer emission intensity of TTMd-ONPs decreases with increasing temperatures whereas the monomer emission is almost independent and can be used as an internal reference. TTMd-ONPs show a great temperature sensitivity (3.4% K-1 at 328 K) and a wide temperature response at ambient conditions with excellent reversibility and high colloidal stability. In addition, TTMd-ONPs are not cytotoxic and their ratiometric outputs are unaffected by changes in the environment. Individual TTMd-ONPs are able to sense temperature changes at the nano-microscale. In vivo thermometry experiments in Caenorhabditis elegans (C. elegans) worms show that TTMd-ONPs can locally monitor internal body temperature changes with spatio-temporal resolution and high sensitivity, offering multiple applications in the biological nanothermometry field.WILEY-V C H VERLAG GMBH2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=23426SMALLISSN: 16136810ISSNe: 16136829reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déuinstname:Fundació Sant Joan de DéuInglésinfo:eu-repo/semantics/openAccessoai:fsjd.fundanetsuite.com:p234262026-05-27T12:37:41Z
dc.title.none.fl_str_mv Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
title Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
spellingShingle Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
Blasi D
Caenorhabditis elegans
excimer emission
in vivo sensing
luminescence
organic radical nanoparticles
ratiometric nanothermometers
trityl radicals
title_short Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
title_full Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
title_fullStr Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
title_full_unstemmed Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
title_sort Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
dc.creator.none.fl_str_mv Blasi D
Gonzalez-Pato N
Rodriguez Rodriguez X
Diez-Zabala I
Srinivasan SY
Camarero N
Esquivias O
Roldán M
Guasch J
Laromaine A
Gorostiza P
Veciana J
Ratera I
author Blasi D
author_facet Blasi D
Gonzalez-Pato N
Rodriguez Rodriguez X
Diez-Zabala I
Srinivasan SY
Camarero N
Esquivias O
Roldán M
Guasch J
Laromaine A
Gorostiza P
Veciana J
Ratera I
author_role author
author2 Gonzalez-Pato N
Rodriguez Rodriguez X
Diez-Zabala I
Srinivasan SY
Camarero N
Esquivias O
Roldán M
Guasch J
Laromaine A
Gorostiza P
Veciana J
Ratera I
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Caenorhabditis elegans
excimer emission
in vivo sensing
luminescence
organic radical nanoparticles
ratiometric nanothermometers
trityl radicals
topic Caenorhabditis elegans
excimer emission
in vivo sensing
luminescence
organic radical nanoparticles
ratiometric nanothermometers
trityl radicals
description Ratiometric fluorescent nanothermometers with near-infrared emission play an important role in in vivo sensing since they can be used as intracellular thermal sensing probes with high spatial resolution and high sensitivity, to investigate cellular functions of interest in diagnosis and therapy, where current approaches are not effective. Herein, the temperature-dependent fluorescence of organic nanoparticles is designed, synthesized, and studied based on the dual emission, generated by monomer and excimer species, of the tris(2,4,6-trichlorophenyl)methyl radical (TTM) doping organic nanoparticles (TTMd-ONPs), made of optically neutral tris(2,4,6-trichlorophenyl)methane (TTM-aH), acting as a matrix. The excimer emission intensity of TTMd-ONPs decreases with increasing temperatures whereas the monomer emission is almost independent and can be used as an internal reference. TTMd-ONPs show a great temperature sensitivity (3.4% K-1 at 328 K) and a wide temperature response at ambient conditions with excellent reversibility and high colloidal stability. In addition, TTMd-ONPs are not cytotoxic and their ratiometric outputs are unaffected by changes in the environment. Individual TTMd-ONPs are able to sense temperature changes at the nano-microscale. In vivo thermometry experiments in Caenorhabditis elegans (C. elegans) worms show that TTMd-ONPs can locally monitor internal body temperature changes with spatio-temporal resolution and high sensitivity, offering multiple applications in the biological nanothermometry field.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=23426
url https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=23426
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv WILEY-V C H VERLAG GMBH
publisher.none.fl_str_mv WILEY-V C H VERLAG GMBH
dc.source.none.fl_str_mv SMALL
ISSN: 16136810
ISSNe: 16136829
reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
instname:Fundació Sant Joan de Déu
instname_str Fundació Sant Joan de Déu
reponame_str r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
collection r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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