MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration

Membrane organization into condensed domains or rafts provides molecular platforms for selective recruitment of proteins. Cell migration is a general process that requires spatiotemporal targeting of Rac1 to membrane rafts. The protein machinery responsible for making rafts competent to recruit Rac1...

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Autores: Aranda, Juan Francisco, Reglero-Real, Natalia, Kremer, Leonor, Marcos-Ramiro, Beatriz, Ruiz-Sáenz, Ana, Calvo Ademuz, Maria, Enrich Bastús, Carles, Correas, Isabel, Millán, Jaime, Alonso, Miguel Ángel
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/25226
Acesso em linha:https://hdl.handle.net/2445/25226
Access Level:acceso abierto
Palavra-chave:Membranes (Biologia)
Citologia
Cytology
Membranes (Biology)
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spelling MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migrationAranda, Juan FranciscoReglero-Real, NataliaKremer, LeonorMarcos-Ramiro, BeatrizRuiz-Sáenz, AnaCalvo Ademuz, MariaEnrich Bastús, CarlesCorreas, IsabelMillán, JaimeAlonso, Miguel ÁngelMembranes (Biologia)CitologiaCytologyMembranes (Biology)Membrane organization into condensed domains or rafts provides molecular platforms for selective recruitment of proteins. Cell migration is a general process that requires spatiotemporal targeting of Rac1 to membrane rafts. The protein machinery responsible for making rafts competent to recruit Rac1 remains elusive. Some members of the MAL family of proteins are involved in specialized processes dependent on this type of membrane. Because condensed membrane domains are a general feature of the plasma membrane of all mammalian cells, we hypothesized that MAL family members with ubiquitous expression and plasma membrane distribution could be involved in the organization of membranes for cell migration. We show that myeloid-associated differentiation marker (MYADM), a protein with unique features within the MAL family, colocalizes with Rac1 in membrane protrusions at the cell surface and distributes in condensed membranes. MYADM knockdown (KD) cells had altered membrane condensation and showed deficient incorporation of Rac1 to membrane raft fractions and, similar to Rac1 KD cells, exhibited reduced cell spreading and migration. Results of rescue-of-function experiments by expression of MYADM or active Rac1L61 in cells knocked down for Rac1 or MYADM, respectively, are consistent with the idea that MYADM and Rac1 act on parallel pathways that lead to similar functional outcomes.American Society for Cell Biology2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/25226Articles publicats en revistes (Biomedicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1091/mbc.E10-11-0910Molecular Biology of the Cell, 2011, vol. 22, núm. 8, p. 1252-1262http://dx.doi.org/10.1091/mbc.E10-11-0910cc-by-nc-sa, (c) Aranda et al., 2011http://creativecommons.org/licenses/cc-by-nc-sa/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/252262026-05-27T06:46:51Z
dc.title.none.fl_str_mv MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration
title MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration
spellingShingle MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration
Aranda, Juan Francisco
Membranes (Biologia)
Citologia
Cytology
Membranes (Biology)
title_short MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration
title_full MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration
title_fullStr MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration
title_full_unstemmed MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration
title_sort MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration
dc.creator.none.fl_str_mv Aranda, Juan Francisco
Reglero-Real, Natalia
Kremer, Leonor
Marcos-Ramiro, Beatriz
Ruiz-Sáenz, Ana
Calvo Ademuz, Maria
Enrich Bastús, Carles
Correas, Isabel
Millán, Jaime
Alonso, Miguel Ángel
author Aranda, Juan Francisco
author_facet Aranda, Juan Francisco
Reglero-Real, Natalia
Kremer, Leonor
Marcos-Ramiro, Beatriz
Ruiz-Sáenz, Ana
Calvo Ademuz, Maria
Enrich Bastús, Carles
Correas, Isabel
Millán, Jaime
Alonso, Miguel Ángel
author_role author
author2 Reglero-Real, Natalia
Kremer, Leonor
Marcos-Ramiro, Beatriz
Ruiz-Sáenz, Ana
Calvo Ademuz, Maria
Enrich Bastús, Carles
Correas, Isabel
Millán, Jaime
Alonso, Miguel Ángel
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Membranes (Biologia)
Citologia
Cytology
Membranes (Biology)
topic Membranes (Biologia)
Citologia
Cytology
Membranes (Biology)
description Membrane organization into condensed domains or rafts provides molecular platforms for selective recruitment of proteins. Cell migration is a general process that requires spatiotemporal targeting of Rac1 to membrane rafts. The protein machinery responsible for making rafts competent to recruit Rac1 remains elusive. Some members of the MAL family of proteins are involved in specialized processes dependent on this type of membrane. Because condensed membrane domains are a general feature of the plasma membrane of all mammalian cells, we hypothesized that MAL family members with ubiquitous expression and plasma membrane distribution could be involved in the organization of membranes for cell migration. We show that myeloid-associated differentiation marker (MYADM), a protein with unique features within the MAL family, colocalizes with Rac1 in membrane protrusions at the cell surface and distributes in condensed membranes. MYADM knockdown (KD) cells had altered membrane condensation and showed deficient incorporation of Rac1 to membrane raft fractions and, similar to Rac1 KD cells, exhibited reduced cell spreading and migration. Results of rescue-of-function experiments by expression of MYADM or active Rac1L61 in cells knocked down for Rac1 or MYADM, respectively, are consistent with the idea that MYADM and Rac1 act on parallel pathways that lead to similar functional outcomes.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/25226
url https://hdl.handle.net/2445/25226
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1091/mbc.E10-11-0910
Molecular Biology of the Cell, 2011, vol. 22, núm. 8, p. 1252-1262
http://dx.doi.org/10.1091/mbc.E10-11-0910
dc.rights.none.fl_str_mv cc-by-nc-sa, (c) Aranda et al., 2011
http://creativecommons.org/licenses/cc-by-nc-sa/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-sa, (c) Aranda et al., 2011
http://creativecommons.org/licenses/cc-by-nc-sa/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Cell Biology
publisher.none.fl_str_mv American Society for Cell Biology
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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