Metabolic engineering of Saccharomyces cerevisiae for hydroxytyrosol overproduction directly from glucose

Hydroxytyrosol (HT) is one of the most powerful dietary antioxidants with numerous applications in different areas, including cosmetics, nutraceuticals and food. In the present work, heterologous hydroxylase complex HpaBC from Escherichia coli was integrated into the Saccharomyces cerevisiae genome...

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Autores: Bisquert, Ricardo, Planells-Cárcel, Andrés, Valera García, Elena, Guillamón, José Manuel, Muñiz Calvo, Sara
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/270796
Acceso en línea:http://hdl.handle.net/10261/270796
https://api.elsevier.com/content/abstract/scopus_id/85117724410
Access Level:acceso abierto
Palabra clave:Hydroxytyrosol
Antioxidants
HpaBC
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spelling Metabolic engineering of Saccharomyces cerevisiae for hydroxytyrosol overproduction directly from glucoseBisquert, RicardoPlanells-Cárcel, AndrésValera García, ElenaGuillamón, José ManuelMuñiz Calvo, SaraHydroxytyrosolAntioxidantsHpaBCHydroxytyrosol (HT) is one of the most powerful dietary antioxidants with numerous applications in different areas, including cosmetics, nutraceuticals and food. In the present work, heterologous hydroxylase complex HpaBC from Escherichia coli was integrated into the Saccharomyces cerevisiae genome in multiple copies. HT productivity was increased by redirecting the metabolic flux towards tyrosol synthesis to avoid exogenous tyrosol or tyrosine supplementation. After evaluating the potential of our selected strain as an HT producer from glucose, we adjusted the medium composition for HT production. The combination of the selected modifications in our engineered strain, combined with culture conditions optimization, resulted in a titre of approximately 375 mg l-1 of HT obtained from shake-flask fermentation using a minimal synthetic-defined medium with 160 g l-1 glucose as the sole carbon source. To the best of our knowledge, this is the highest HT concentration produced by an engineered S. cerevisiae strain.This work was supported by the Spanish Ministry of Science, Innovation and Universities through the grants AGL2016-77505-C3-1-R and PID2019-108722RB-C31, awarded to JMG.Peer reviewedSociety for Applied MicrobiologyMinisterio de Ciencia, Innovación y Universidades (España)0000-0001-6381-88520000-0001-5414-07870000-0003-4689-6589Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/270796https://api.elsevier.com/content/abstract/scopus_id/85117724410reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108722RB-C31Microbial biotechnologyhttps://doi.org/10.1111/1751-7915.13957Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2707962026-05-22T06:33:51Z
dc.title.none.fl_str_mv Metabolic engineering of Saccharomyces cerevisiae for hydroxytyrosol overproduction directly from glucose
title Metabolic engineering of Saccharomyces cerevisiae for hydroxytyrosol overproduction directly from glucose
spellingShingle Metabolic engineering of Saccharomyces cerevisiae for hydroxytyrosol overproduction directly from glucose
Bisquert, Ricardo
Hydroxytyrosol
Antioxidants
HpaBC
title_short Metabolic engineering of Saccharomyces cerevisiae for hydroxytyrosol overproduction directly from glucose
title_full Metabolic engineering of Saccharomyces cerevisiae for hydroxytyrosol overproduction directly from glucose
title_fullStr Metabolic engineering of Saccharomyces cerevisiae for hydroxytyrosol overproduction directly from glucose
title_full_unstemmed Metabolic engineering of Saccharomyces cerevisiae for hydroxytyrosol overproduction directly from glucose
title_sort Metabolic engineering of Saccharomyces cerevisiae for hydroxytyrosol overproduction directly from glucose
dc.creator.none.fl_str_mv Bisquert, Ricardo
Planells-Cárcel, Andrés
Valera García, Elena
Guillamón, José Manuel
Muñiz Calvo, Sara
author Bisquert, Ricardo
author_facet Bisquert, Ricardo
Planells-Cárcel, Andrés
Valera García, Elena
Guillamón, José Manuel
Muñiz Calvo, Sara
author_role author
author2 Planells-Cárcel, Andrés
Valera García, Elena
Guillamón, José Manuel
Muñiz Calvo, Sara
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
0000-0001-6381-8852
0000-0001-5414-0787
0000-0003-4689-6589
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Hydroxytyrosol
Antioxidants
HpaBC
topic Hydroxytyrosol
Antioxidants
HpaBC
description Hydroxytyrosol (HT) is one of the most powerful dietary antioxidants with numerous applications in different areas, including cosmetics, nutraceuticals and food. In the present work, heterologous hydroxylase complex HpaBC from Escherichia coli was integrated into the Saccharomyces cerevisiae genome in multiple copies. HT productivity was increased by redirecting the metabolic flux towards tyrosol synthesis to avoid exogenous tyrosol or tyrosine supplementation. After evaluating the potential of our selected strain as an HT producer from glucose, we adjusted the medium composition for HT production. The combination of the selected modifications in our engineered strain, combined with culture conditions optimization, resulted in a titre of approximately 375 mg l-1 of HT obtained from shake-flask fermentation using a minimal synthetic-defined medium with 160 g l-1 glucose as the sole carbon source. To the best of our knowledge, this is the highest HT concentration produced by an engineered S. cerevisiae strain.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/270796
https://api.elsevier.com/content/abstract/scopus_id/85117724410
url http://hdl.handle.net/10261/270796
https://api.elsevier.com/content/abstract/scopus_id/85117724410
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108722RB-C31
Microbial biotechnology
https://doi.org/10.1111/1751-7915.13957
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Society for Applied Microbiology
publisher.none.fl_str_mv Society for Applied Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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