Minor Splicing Factors Zrsr1 and Zrsr2 Are Essential for Early Embryo Development and 2-Cell-Like Conversion

15 Pág.

Detalles Bibliográficos
Autores: Gómez-Redondo, Isabel, Ramos Ibeas, Priscila, Pericuesta Camacho, Eva, Fernández González, Raúl, Laguna-Barraza, Ricardo, Gutiérrez-Adán, Alfonso
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/340397
Acceso en línea:http://hdl.handle.net/10261/340397
https://api.elsevier.com/content/abstract/scopus_id/85086221685
Access Level:acceso abierto
Palabra clave:2C-like cells
U12 introns
Zrsr1
Zrsr2
Zygotic gene activation
id ES_6e6f03b4bb6099aaffc0d4851cecd5e7
oai_identifier_str oai:digital.csic.es:10261/340397
network_acronym_str ES
network_name_str España
repository_id_str
spelling Minor Splicing Factors Zrsr1 and Zrsr2 Are Essential for Early Embryo Development and 2-Cell-Like ConversionGómez-Redondo, IsabelRamos Ibeas, PriscilaPericuesta Camacho, EvaFernández González, RaúlLaguna-Barraza, RicardoGutiérrez-Adán, Alfonso2C-like cellsU12 intronsZrsr1Zrsr2Zygotic gene activation15 Pág.Minor splicing plays an important role in vertebrate development. Zrsr1 and Zrsr2 paralog genes have essential roles in alternative splicing, mainly participating in the recognition of minor (U12) introns. To further explore their roles during early embryo development, we produced Zrsr1mu and Zrsr2mu mutant mice, containing truncating mutations within the second zinc finger domain. Both homozygous mutant mice were viable with a normal lifespan. When we crossed a homozygous Zrsr2mu/mu female with Zrsr1mu/mu male, the double heterozygotes were non-viable, giving rise to embryos that stopped developing mainly between the 2- and 4-cell stages, just after zygotic gene activation. RNA-seq analysis of Zrsr1/2mu 2-cell embryos showed altered gene and isoform expression of thousands of genes enriched in gene ontology terms and biological pathways related to ribosome, RNA transport, spliceosome, and essential zygotic gene activation steps. Alternative splicing was analyzed, showing a significant increase in intron retention in both U2 and U12 intron-containing genes related to cell cycle and mitotic nuclear division. Remarkably, both Zrsr1 and Zrsr2 were required for the conversion of mouse-induced pluripotent stem cells into 2C-like cells. According to our results, Zrsr1 or Zrsr2 are necessary for ZGA and both are indispensable for the conversion of induced pluripotent stem cells into 2C-like cells.This work was funded by grants AGL2015-66145-R and RTI2018-093548-B-I00 from the Spanish Ministry of Science and Innovation. I.G.-R. was supported by a predoctoral fellowship from the same ministry (BES-2016- 077794). P.R.-I. was supported by a Talent Attraction Postdoctoral Fellowship from the Madrid Community (2017-T2/BIO-5182) and by a Ramón y Cajal contract from the Spanish Ministry of Science and Innovation (RYC2018-025666-I).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Agencia Estatal de Investigación (España)Comunidad de MadridMinisterio de Ciencia, Innovación y Universidades (España)Gómez-Redondo, Isabel [0000-0002-9319-7580]Ramos Ibeas, Priscila [0000-0003-4578-0189]Pericuesta Camacho, Eva [0000-0001-9387-3270]Fernández González, Raúl [0000-0003-1989-2945]Gutiérrez-Adán, Alfonso [0000-0001-9893-9179]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/340397https://api.elsevier.com/content/abstract/scopus_id/85086221685reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//AGL2015-66145-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093548-B-I00info:eu-repo/grantAgreement/MINECO//BES-2016-077794S2017-T2/BIO-5182info:eu-repo/grantAgreement/MICIU//RYC2018-025666-IDepartamento de Reproducción Animal​ https://doi.org/10.3390/ijms21114115Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3403972026-05-22T06:33:51Z
dc.title.none.fl_str_mv Minor Splicing Factors Zrsr1 and Zrsr2 Are Essential for Early Embryo Development and 2-Cell-Like Conversion
title Minor Splicing Factors Zrsr1 and Zrsr2 Are Essential for Early Embryo Development and 2-Cell-Like Conversion
spellingShingle Minor Splicing Factors Zrsr1 and Zrsr2 Are Essential for Early Embryo Development and 2-Cell-Like Conversion
Gómez-Redondo, Isabel
2C-like cells
U12 introns
Zrsr1
Zrsr2
Zygotic gene activation
title_short Minor Splicing Factors Zrsr1 and Zrsr2 Are Essential for Early Embryo Development and 2-Cell-Like Conversion
title_full Minor Splicing Factors Zrsr1 and Zrsr2 Are Essential for Early Embryo Development and 2-Cell-Like Conversion
title_fullStr Minor Splicing Factors Zrsr1 and Zrsr2 Are Essential for Early Embryo Development and 2-Cell-Like Conversion
title_full_unstemmed Minor Splicing Factors Zrsr1 and Zrsr2 Are Essential for Early Embryo Development and 2-Cell-Like Conversion
title_sort Minor Splicing Factors Zrsr1 and Zrsr2 Are Essential for Early Embryo Development and 2-Cell-Like Conversion
dc.creator.none.fl_str_mv Gómez-Redondo, Isabel
Ramos Ibeas, Priscila
Pericuesta Camacho, Eva
Fernández González, Raúl
Laguna-Barraza, Ricardo
Gutiérrez-Adán, Alfonso
author Gómez-Redondo, Isabel
author_facet Gómez-Redondo, Isabel
Ramos Ibeas, Priscila
Pericuesta Camacho, Eva
Fernández González, Raúl
Laguna-Barraza, Ricardo
Gutiérrez-Adán, Alfonso
author_role author
author2 Ramos Ibeas, Priscila
Pericuesta Camacho, Eva
Fernández González, Raúl
Laguna-Barraza, Ricardo
Gutiérrez-Adán, Alfonso
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
Comunidad de Madrid
Ministerio de Ciencia, Innovación y Universidades (España)
Gómez-Redondo, Isabel [0000-0002-9319-7580]
Ramos Ibeas, Priscila [0000-0003-4578-0189]
Pericuesta Camacho, Eva [0000-0001-9387-3270]
Fernández González, Raúl [0000-0003-1989-2945]
Gutiérrez-Adán, Alfonso [0000-0001-9893-9179]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv 2C-like cells
U12 introns
Zrsr1
Zrsr2
Zygotic gene activation
topic 2C-like cells
U12 introns
Zrsr1
Zrsr2
Zygotic gene activation
description 15 Pág.
publishDate 2020
dc.date.none.fl_str_mv 2020
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/340397
https://api.elsevier.com/content/abstract/scopus_id/85086221685
url http://hdl.handle.net/10261/340397
https://api.elsevier.com/content/abstract/scopus_id/85086221685
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//AGL2015-66145-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093548-B-I00
info:eu-repo/grantAgreement/MINECO//BES-2016-077794
S2017-T2/BIO-5182
info:eu-repo/grantAgreement/MICIU//RYC2018-025666-I
Departamento de Reproducción Animal​ 
https://doi.org/10.3390/ijms21114115
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410423694950400
score 15,811543

Similares en LA Referencia