Increased synovial immunohistochemistry reactivity of TGF-?1 in erosive peripheral psoriatic arthritis

BACKGROUND: Immune and non-immune cells contribute to the pathology of chronic arthritis, and they can contribute to tissue remodeling and repair as well as disease pathogenesis. The present research aimed to analyze inflammation and bone destruction/regeneration biomarkers in patients with psoriati...

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Autores: Pinto Tasende, José Antonio, Fernández Moreno, Mercedes, Vázquez Mosquera, María Eugenia, Fernandez López, Jesus Carlos, Oreiro Villar, Natividad, De Toro Santos, Francisco Javier, Blanco García, Francisco
Formato: artículo
Fecha de publicación:2023
País:España
Recursos:Servizo Galego de Saúde (SERGAS)
Repositorio:RUNA. Repositorio da Consellería de Sanidade e Sergas
OAI Identifier:oai:runa.sergas.gal:20.500.11940/21634
Acesso em linha:https://portalcientifico.sergas.gal//documentos/6433d25fe8f2fa0e62f2b2df
http://hdl.handle.net/20.500.11940/21634
Access Level:acceso abierto
Palavra-chave:Humans
Arthritis, Psoriatic
Transforming Growth Factor beta1
Interleukin-17
Synovial Fluid
Immunohistochemistry
Synovial Membrane
RNA, Messenger
AS A Coruña
INIBIC
CHUAC
AS Santiago
CHUS
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spelling Increased synovial immunohistochemistry reactivity of TGF-?1 in erosive peripheral psoriatic arthritisPinto Tasende, José AntonioFernández Moreno, MercedesVázquez Mosquera, María EugeniaFernandez López, Jesus CarlosOreiro Villar, NatividadDe Toro Santos, Francisco JavierBlanco García, FranciscoHumansArthritis, PsoriaticTransforming Growth Factor beta1Interleukin-17Synovial FluidImmunohistochemistrySynovial MembraneRNA, MessengerAS A CoruñaINIBICAS A CoruñaCHUACAS SantiagoCHUSAS A CoruñaCHUACAS A CoruñaCHUACAS A CoruñaINIBICAS A CoruñaCHUACBACKGROUND: Immune and non-immune cells contribute to the pathology of chronic arthritis, and they can contribute to tissue remodeling and repair as well as disease pathogenesis. The present research aimed to analyze inflammation and bone destruction/regeneration biomarkers in patients with psoriatic arthritis (PsA), rheumatoid arthritis (RA), osteoarthritis (OA), and ankylosing spondylitis (AS). METHODS: Samples were obtained from the inflamed knee of patients with knee arthritis who had been referred for undergoing arthroscopies. The synovial membrane was processed for pathological description, IHC analysis, and quantification of mRNA expression ratio by qRT-PCR. Serum levels of TGF-?1, IL-23, IL-6, IL-17 A, IL-22, Dkk1, Sclerostin, BMP2, BMP4, Wnt1, and Wnt5a were measured by ELISA. All these data were analyzed and compared with the demographic, clinical, blood tests, and radiological characteristics of the patients. RESULTS: The synovial membrane samples were obtained from 42 patients for IHC, extraction, and purification of RNA for synovial mRNA expression analysis, and serum for measuring protein levels from 38 patients. IHC reactivity for TGF-?1 in the synovial tissue was higher in patients with psoriatic arthritis (p 0.036) and was positively correlated with IL-17 A (r = 0.389, p = 0.012), and Dkk1 (r = 0.388, p = 0.012). Gene expression of the IL-17 A was higher in PsA patients (p = 0.018) and was positively correlated with Dkk1 (r = 0.424, p = 0.022) and negatively correlated with BMP2 (r = -0.396, p = 0.033) and BMP4 (r = -0.472, p = 0.010). It was observed that IHC reactivity for TGF-?1 was higher in patients with erosive PsA (p = 0.024). CONCLUSIONS: The IHC reactivity of TGF-?1 in synovial tissue was higher in patients with erosive psoriatic arthritis, and TGF-?1 was in relation to higher levels of gene expression of IL-17 A and Dkk1.Grant (PI11/00390) from Plan Nacional de Investigacion Cientifica, Desarrollo e InnovacionTecnologica 2008-2011 and co-financed by the ISCIII-Subdireccion General de Evaluacion y Fomento de la Investigacion - Fondo Europeo de Desarrollo Regional (FEDER).2023info:eu-repo/semantics/articlehttps://portalcientifico.sergas.gal//documentos/6433d25fe8f2fa0e62f2b2dfhttp://hdl.handle.net/20.500.11940/21634reponame:RUNA. Repositorio da Consellería de Sanidade e Sergasinstname:Servizo Galego de Saúde (SERGAS)Ingléshttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:runa.sergas.gal:20.500.11940/216342026-06-12T08:40:47Z
dc.title.none.fl_str_mv Increased synovial immunohistochemistry reactivity of TGF-?1 in erosive peripheral psoriatic arthritis
title Increased synovial immunohistochemistry reactivity of TGF-?1 in erosive peripheral psoriatic arthritis
spellingShingle Increased synovial immunohistochemistry reactivity of TGF-?1 in erosive peripheral psoriatic arthritis
Pinto Tasende, José Antonio
Humans
Arthritis, Psoriatic
Transforming Growth Factor beta1
Interleukin-17
Synovial Fluid
Immunohistochemistry
Synovial Membrane
RNA, Messenger
AS A Coruña
INIBIC
AS A Coruña
CHUAC
AS Santiago
CHUS
AS A Coruña
CHUAC
AS A Coruña
CHUAC
AS A Coruña
INIBIC
AS A Coruña
CHUAC
title_short Increased synovial immunohistochemistry reactivity of TGF-?1 in erosive peripheral psoriatic arthritis
title_full Increased synovial immunohistochemistry reactivity of TGF-?1 in erosive peripheral psoriatic arthritis
title_fullStr Increased synovial immunohistochemistry reactivity of TGF-?1 in erosive peripheral psoriatic arthritis
title_full_unstemmed Increased synovial immunohistochemistry reactivity of TGF-?1 in erosive peripheral psoriatic arthritis
title_sort Increased synovial immunohistochemistry reactivity of TGF-?1 in erosive peripheral psoriatic arthritis
dc.creator.none.fl_str_mv Pinto Tasende, José Antonio
Fernández Moreno, Mercedes
Vázquez Mosquera, María Eugenia
Fernandez López, Jesus Carlos
Oreiro Villar, Natividad
De Toro Santos, Francisco Javier
Blanco García, Francisco
author Pinto Tasende, José Antonio
author_facet Pinto Tasende, José Antonio
Fernández Moreno, Mercedes
Vázquez Mosquera, María Eugenia
Fernandez López, Jesus Carlos
Oreiro Villar, Natividad
De Toro Santos, Francisco Javier
Blanco García, Francisco
author_role author
author2 Fernández Moreno, Mercedes
Vázquez Mosquera, María Eugenia
Fernandez López, Jesus Carlos
Oreiro Villar, Natividad
De Toro Santos, Francisco Javier
Blanco García, Francisco
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Humans
Arthritis, Psoriatic
Transforming Growth Factor beta1
Interleukin-17
Synovial Fluid
Immunohistochemistry
Synovial Membrane
RNA, Messenger
AS A Coruña
INIBIC
AS A Coruña
CHUAC
AS Santiago
CHUS
AS A Coruña
CHUAC
AS A Coruña
CHUAC
AS A Coruña
INIBIC
AS A Coruña
CHUAC
topic Humans
Arthritis, Psoriatic
Transforming Growth Factor beta1
Interleukin-17
Synovial Fluid
Immunohistochemistry
Synovial Membrane
RNA, Messenger
AS A Coruña
INIBIC
AS A Coruña
CHUAC
AS Santiago
CHUS
AS A Coruña
CHUAC
AS A Coruña
CHUAC
AS A Coruña
INIBIC
AS A Coruña
CHUAC
description BACKGROUND: Immune and non-immune cells contribute to the pathology of chronic arthritis, and they can contribute to tissue remodeling and repair as well as disease pathogenesis. The present research aimed to analyze inflammation and bone destruction/regeneration biomarkers in patients with psoriatic arthritis (PsA), rheumatoid arthritis (RA), osteoarthritis (OA), and ankylosing spondylitis (AS). METHODS: Samples were obtained from the inflamed knee of patients with knee arthritis who had been referred for undergoing arthroscopies. The synovial membrane was processed for pathological description, IHC analysis, and quantification of mRNA expression ratio by qRT-PCR. Serum levels of TGF-?1, IL-23, IL-6, IL-17 A, IL-22, Dkk1, Sclerostin, BMP2, BMP4, Wnt1, and Wnt5a were measured by ELISA. All these data were analyzed and compared with the demographic, clinical, blood tests, and radiological characteristics of the patients. RESULTS: The synovial membrane samples were obtained from 42 patients for IHC, extraction, and purification of RNA for synovial mRNA expression analysis, and serum for measuring protein levels from 38 patients. IHC reactivity for TGF-?1 in the synovial tissue was higher in patients with psoriatic arthritis (p 0.036) and was positively correlated with IL-17 A (r = 0.389, p = 0.012), and Dkk1 (r = 0.388, p = 0.012). Gene expression of the IL-17 A was higher in PsA patients (p = 0.018) and was positively correlated with Dkk1 (r = 0.424, p = 0.022) and negatively correlated with BMP2 (r = -0.396, p = 0.033) and BMP4 (r = -0.472, p = 0.010). It was observed that IHC reactivity for TGF-?1 was higher in patients with erosive PsA (p = 0.024). CONCLUSIONS: The IHC reactivity of TGF-?1 in synovial tissue was higher in patients with erosive psoriatic arthritis, and TGF-?1 was in relation to higher levels of gene expression of IL-17 A and Dkk1.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://portalcientifico.sergas.gal//documentos/6433d25fe8f2fa0e62f2b2df
http://hdl.handle.net/20.500.11940/21634
url https://portalcientifico.sergas.gal//documentos/6433d25fe8f2fa0e62f2b2df
http://hdl.handle.net/20.500.11940/21634
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:RUNA. Repositorio da Consellería de Sanidade e Sergas
instname:Servizo Galego de Saúde (SERGAS)
instname_str Servizo Galego de Saúde (SERGAS)
reponame_str RUNA. Repositorio da Consellería de Sanidade e Sergas
collection RUNA. Repositorio da Consellería de Sanidade e Sergas
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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