Physiological Translocation of Lactic Acid Bacteria during Pregnancy Contributes to the Composition of the Milk Microbiota in Mice

The human milk microbiota is a complex and diverse ecosystem that seems to play a relevant role in the mother-to-infant transmission of microorganisms during early life. Bacteria present in human milk may arise from different sources, and recent studies suggest that at least some of them may be orig...

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Detalhes bibliográficos
Autores: Andrés Leo, Javier de, Jiménez Quintana, Esther Antonia, Chico-Calero, Isabel, Fresno, Manuel, Fernández Álvarez, Leonides, Rodríguez Gómez, Juan Miguel
Formato: artículo
Fecha de publicación:2017
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/19146
Acesso em linha:https://hdl.handle.net/20.500.14352/19146
Access Level:acceso abierto
Palavra-chave:human milk
translocation
Lactobacillus salivarius
lux
bioluminescence
pregnancy
lactation
Ginecología y obstetricia
3201.08 Ginecología
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spelling Physiological Translocation of Lactic Acid Bacteria during Pregnancy Contributes to the Composition of the Milk Microbiota in MiceAndrés Leo, Javier deJiménez Quintana, Esther AntoniaChico-Calero, IsabelFresno, ManuelFernández Álvarez, LeonidesRodríguez Gómez, Juan Miguelhuman milktranslocationLactobacillus salivariusluxbioluminescencepregnancylactationGinecología y obstetricia3201.08 GinecologíaThe human milk microbiota is a complex and diverse ecosystem that seems to play a relevant role in the mother-to-infant transmission of microorganisms during early life. Bacteria present in human milk may arise from different sources, and recent studies suggest that at least some of them may be originally present in the maternal digestive tract and may reach the mammary gland through an endogenous route during pregnancy and lactation. The objective of this work was to elucidate whether some lactic acid bacteria are able to translocate and colonize the mammary gland and milk. For this purpose, two lactic acid bacteria strains (Lactococcus lactis MG1614 and Lactobacillus salivarius PS2) were transformed with a plasmid containing the lux genes; subsequently, the transformed strains were orally administered to pregnant mice. The murine model allowed the visualization, isolation, and Polymerase Chain Reaction (PCR)-detection of the transformed bacteria in different body locations, including mammary tissue and milk, reinforcing the hypothesis that physiological translocation of maternal bacteria during pregnancy and lactation may contribute to the composition of the mammary and milk microbiota.MDPIUniversidad Complutense de Madrid20172017-12-2320172017-12-23journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/19146reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/191462026-06-02T12:44:21Z
dc.title.none.fl_str_mv Physiological Translocation of Lactic Acid Bacteria during Pregnancy Contributes to the Composition of the Milk Microbiota in Mice
title Physiological Translocation of Lactic Acid Bacteria during Pregnancy Contributes to the Composition of the Milk Microbiota in Mice
spellingShingle Physiological Translocation of Lactic Acid Bacteria during Pregnancy Contributes to the Composition of the Milk Microbiota in Mice
Andrés Leo, Javier de
human milk
translocation
Lactobacillus salivarius
lux
bioluminescence
pregnancy
lactation
Ginecología y obstetricia
3201.08 Ginecología
title_short Physiological Translocation of Lactic Acid Bacteria during Pregnancy Contributes to the Composition of the Milk Microbiota in Mice
title_full Physiological Translocation of Lactic Acid Bacteria during Pregnancy Contributes to the Composition of the Milk Microbiota in Mice
title_fullStr Physiological Translocation of Lactic Acid Bacteria during Pregnancy Contributes to the Composition of the Milk Microbiota in Mice
title_full_unstemmed Physiological Translocation of Lactic Acid Bacteria during Pregnancy Contributes to the Composition of the Milk Microbiota in Mice
title_sort Physiological Translocation of Lactic Acid Bacteria during Pregnancy Contributes to the Composition of the Milk Microbiota in Mice
dc.creator.none.fl_str_mv Andrés Leo, Javier de
Jiménez Quintana, Esther Antonia
Chico-Calero, Isabel
Fresno, Manuel
Fernández Álvarez, Leonides
Rodríguez Gómez, Juan Miguel
author Andrés Leo, Javier de
author_facet Andrés Leo, Javier de
Jiménez Quintana, Esther Antonia
Chico-Calero, Isabel
Fresno, Manuel
Fernández Álvarez, Leonides
Rodríguez Gómez, Juan Miguel
author_role author
author2 Jiménez Quintana, Esther Antonia
Chico-Calero, Isabel
Fresno, Manuel
Fernández Álvarez, Leonides
Rodríguez Gómez, Juan Miguel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv human milk
translocation
Lactobacillus salivarius
lux
bioluminescence
pregnancy
lactation
Ginecología y obstetricia
3201.08 Ginecología
topic human milk
translocation
Lactobacillus salivarius
lux
bioluminescence
pregnancy
lactation
Ginecología y obstetricia
3201.08 Ginecología
description The human milk microbiota is a complex and diverse ecosystem that seems to play a relevant role in the mother-to-infant transmission of microorganisms during early life. Bacteria present in human milk may arise from different sources, and recent studies suggest that at least some of them may be originally present in the maternal digestive tract and may reach the mammary gland through an endogenous route during pregnancy and lactation. The objective of this work was to elucidate whether some lactic acid bacteria are able to translocate and colonize the mammary gland and milk. For this purpose, two lactic acid bacteria strains (Lactococcus lactis MG1614 and Lactobacillus salivarius PS2) were transformed with a plasmid containing the lux genes; subsequently, the transformed strains were orally administered to pregnant mice. The murine model allowed the visualization, isolation, and Polymerase Chain Reaction (PCR)-detection of the transformed bacteria in different body locations, including mammary tissue and milk, reinforcing the hypothesis that physiological translocation of maternal bacteria during pregnancy and lactation may contribute to the composition of the mammary and milk microbiota.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-12-23
2017
2017-12-23
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/19146
url https://hdl.handle.net/20.500.14352/19146
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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