A Combination of Proteomic Approaches Identifies A Panel of Circulating Extracellular Vesicle Proteins Related to the Risk of Suffering Cardiovascular Disease in Obese Patients

Plasma-derived extracellular vesicles (EVs) have been extensively described as putative biomarkers in different diseases. Interestingly, increased levels of EVs subpopulations are well known to associate with obesity. The goal of this study is to identify EVs-derived biomarkers in plasma from obese...

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Autores: Barrachina, Maria N., Sueiro, Aurelio M., Casas, Vanessa, Izquierdo, Irene, Hermida-Nogueira, Lidia, Guitián, Esteban, Casanueva, Felipe F., Abián, Joaquín, Carrascal, Montserrat, Pardo, María, García, Ángel
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/201232
Acceso en línea:http://hdl.handle.net/10261/201232
Access Level:acceso abierto
Palabra clave:Biomarkers
Extracellular vesicles
Label-free quantitation
Obesity
Plasma
2D-DIGE
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spelling A Combination of Proteomic Approaches Identifies A Panel of Circulating Extracellular Vesicle Proteins Related to the Risk of Suffering Cardiovascular Disease in Obese PatientsBarrachina, Maria N.Sueiro, Aurelio M.Casas, VanessaIzquierdo, IreneHermida-Nogueira, LidiaGuitián, EstebanCasanueva, Felipe F.Abián, JoaquínCarrascal, MontserratPardo, MaríaGarcía, ÁngelBiomarkersExtracellular vesiclesLabel-free quantitationObesityPlasma2D-DIGEPlasma-derived extracellular vesicles (EVs) have been extensively described as putative biomarkers in different diseases. Interestingly, increased levels of EVs subpopulations are well known to associate with obesity. The goal of this study is to identify EVs-derived biomarkers in plasma from obese patients in order to predict the development of pathological events associated with obesity. Samples are obtained from 22 obese patients and their lean-matched controls are divided into two cohorts: one for a 2D fluorescence difference gel electrophoresis (2D-DIGE)-based study, and the other one for a label free LC–MS/MS-based approach. EVs are isolated following a serial ultracentrifugation protocol. Twenty-two and 23 differentially regulated features are detected from 2D-DIGE and label free LC–MS/MS, respectively; most of them involve in the coagulation and complement cascades. Remarkably, there is an upregulation of complement C4, complement C3, and fibrinogen in obese patients following both approaches, the latter two also validated by 2D-western-blotting in an independent cohort. These results correlate with a proinflammatory and prothrombotic state of those individuals. On the other hand, a downregulation of adiponectin leading to an increased risk of suffering cardiovascular diseases has been shown. The results suggest the relevance of plasma-derived-EVs proteins as a source of potential biomarkers for the development of atherothrombotic events in obesity.This work was supported by the Spanish Ministry of Economy and Competitiveness; grant No. SAF2016-79662-R), co-funded by the European Regional Development Fund. Financial support from the Conseller ́ıa de Cultura, Educaci ́on e Ordenaci ́on Universitaria, Xunta de Galicia (Centro Singular de investigaci ́on de Galicia accreditation 2016–2019,ED431G/05), and the European Regional Development Fund is also grate-fully acknowledged. The Proteomics Laboratory CSIC/UAB is a member ofProteored, PRB2-ISCIII and is supported by Grant PT13/0001, of the PEI+D+i 2013–2016, funded by ISCIII and FEDER.John Wiley & SonsMinisterio de Economía y Competitividad (España)European CommissionXunta de GaliciaInstituto de Salud Carlos IIIConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/201232reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-79662-Rhttp://dx.doi.org/10.1002/pmic.201800248Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2012322026-05-22T06:33:51Z
dc.title.none.fl_str_mv A Combination of Proteomic Approaches Identifies A Panel of Circulating Extracellular Vesicle Proteins Related to the Risk of Suffering Cardiovascular Disease in Obese Patients
title A Combination of Proteomic Approaches Identifies A Panel of Circulating Extracellular Vesicle Proteins Related to the Risk of Suffering Cardiovascular Disease in Obese Patients
spellingShingle A Combination of Proteomic Approaches Identifies A Panel of Circulating Extracellular Vesicle Proteins Related to the Risk of Suffering Cardiovascular Disease in Obese Patients
Barrachina, Maria N.
Biomarkers
Extracellular vesicles
Label-free quantitation
Obesity
Plasma
2D-DIGE
title_short A Combination of Proteomic Approaches Identifies A Panel of Circulating Extracellular Vesicle Proteins Related to the Risk of Suffering Cardiovascular Disease in Obese Patients
title_full A Combination of Proteomic Approaches Identifies A Panel of Circulating Extracellular Vesicle Proteins Related to the Risk of Suffering Cardiovascular Disease in Obese Patients
title_fullStr A Combination of Proteomic Approaches Identifies A Panel of Circulating Extracellular Vesicle Proteins Related to the Risk of Suffering Cardiovascular Disease in Obese Patients
title_full_unstemmed A Combination of Proteomic Approaches Identifies A Panel of Circulating Extracellular Vesicle Proteins Related to the Risk of Suffering Cardiovascular Disease in Obese Patients
title_sort A Combination of Proteomic Approaches Identifies A Panel of Circulating Extracellular Vesicle Proteins Related to the Risk of Suffering Cardiovascular Disease in Obese Patients
dc.creator.none.fl_str_mv Barrachina, Maria N.
Sueiro, Aurelio M.
Casas, Vanessa
Izquierdo, Irene
Hermida-Nogueira, Lidia
Guitián, Esteban
Casanueva, Felipe F.
Abián, Joaquín
Carrascal, Montserrat
Pardo, María
García, Ángel
author Barrachina, Maria N.
author_facet Barrachina, Maria N.
Sueiro, Aurelio M.
Casas, Vanessa
Izquierdo, Irene
Hermida-Nogueira, Lidia
Guitián, Esteban
Casanueva, Felipe F.
Abián, Joaquín
Carrascal, Montserrat
Pardo, María
García, Ángel
author_role author
author2 Sueiro, Aurelio M.
Casas, Vanessa
Izquierdo, Irene
Hermida-Nogueira, Lidia
Guitián, Esteban
Casanueva, Felipe F.
Abián, Joaquín
Carrascal, Montserrat
Pardo, María
García, Ángel
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Xunta de Galicia
Instituto de Salud Carlos III
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biomarkers
Extracellular vesicles
Label-free quantitation
Obesity
Plasma
2D-DIGE
topic Biomarkers
Extracellular vesicles
Label-free quantitation
Obesity
Plasma
2D-DIGE
description Plasma-derived extracellular vesicles (EVs) have been extensively described as putative biomarkers in different diseases. Interestingly, increased levels of EVs subpopulations are well known to associate with obesity. The goal of this study is to identify EVs-derived biomarkers in plasma from obese patients in order to predict the development of pathological events associated with obesity. Samples are obtained from 22 obese patients and their lean-matched controls are divided into two cohorts: one for a 2D fluorescence difference gel electrophoresis (2D-DIGE)-based study, and the other one for a label free LC–MS/MS-based approach. EVs are isolated following a serial ultracentrifugation protocol. Twenty-two and 23 differentially regulated features are detected from 2D-DIGE and label free LC–MS/MS, respectively; most of them involve in the coagulation and complement cascades. Remarkably, there is an upregulation of complement C4, complement C3, and fibrinogen in obese patients following both approaches, the latter two also validated by 2D-western-blotting in an independent cohort. These results correlate with a proinflammatory and prothrombotic state of those individuals. On the other hand, a downregulation of adiponectin leading to an increased risk of suffering cardiovascular diseases has been shown. The results suggest the relevance of plasma-derived-EVs proteins as a source of potential biomarkers for the development of atherothrombotic events in obesity.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/201232
url http://hdl.handle.net/10261/201232
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-79662-R
http://dx.doi.org/10.1002/pmic.201800248
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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