Comparative toxicity and endocrine disruption potential of urban and rural atmospheric organic PM1 in JEG-3 human placental cells

Outdoor ambient air particulate matter and air pollution are related to adverse effects on human health. The present study assesses the cytotoxicity and ability to disrupt aromatase activity of organic PM1 extracts from rural and urban areas at equivalent air volumes from 2 to 30 m3, in human placen...

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Detalles Bibliográficos
Autores: van Drooge, Barend L., Marqueño, Anna, Grimalt, Joan O., Fernández Ramón, M. Pilar, Porte, Cinta
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/344927
Acceso en línea:http://hdl.handle.net/10261/344927
https://api.elsevier.com/content/abstract/scopus_id/85021403983
Access Level:acceso abierto
Palabra clave:Organic air pollution
PM(1)
Biomass burning
Cytotoxicity
Endocrine disruption
Human placental JEG 3 cells
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Descripción
Sumario:Outdoor ambient air particulate matter and air pollution are related to adverse effects on human health. The present study assesses the cytotoxicity and ability to disrupt aromatase activity of organic PM1 extracts from rural and urban areas at equivalent air volumes from 2 to 30 m3, in human placental JEG-3 cells. Samples were chemically analyzed for particle bounded organic compounds with endocrine disrupting potential, i.e. PAH, O-PAH, phthalate esters, but also for organic molecular tracer compounds for the emission source identification. Rural samples collected in winter were cytotoxic at the highest concentration tested and strongly inhibited aromatase activity in JEG-3 cells. No cytotoxicity was detected in summer samples from the rural site and the urban samples, while aromatase activity was moderately inhibited in these samples. In the urban area, the street site samples, collected close to intensive traffic, showed stronger inhibition of aromatase activity than the samples simultaneously collected at a roof site, 50 m above ground level. The cytotoxicity and endocrine disruption potential of the samples were linked to combustion products, i.e. PAH and O-PAH, especially from biomass burning in the rural site in winter.