Local assessment of the immunohistochemical expression of Foxp3+regulatory T lymphocytes in the different pathological forms associated with bovine paratuberculosis

11 páginas, 3 figuras, 2 tablas.

Detalles Bibliográficos
Autores: Zapico, David, Espinosa Cerrato, José, Fernández Fernández, Miguel, Criado, M., Arteche-Villasol, Noive, Pérez Pérez, Valentín
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/279451
Acceso en línea:http://hdl.handle.net/10261/279451
Access Level:acceso abierto
Palabra clave:Mycobacterium avium subsp
Paratuberculosis
Foxp3+
Intestinal tissue
Type of lesion
Regulatory T lymphocytes
Cattle
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dc.title.none.fl_str_mv Local assessment of the immunohistochemical expression of Foxp3+regulatory T lymphocytes in the different pathological forms associated with bovine paratuberculosis
title Local assessment of the immunohistochemical expression of Foxp3+regulatory T lymphocytes in the different pathological forms associated with bovine paratuberculosis
spellingShingle Local assessment of the immunohistochemical expression of Foxp3+regulatory T lymphocytes in the different pathological forms associated with bovine paratuberculosis
Zapico, David
Mycobacterium avium subsp
Paratuberculosis
Foxp3+
Intestinal tissue
Type of lesion
Regulatory T lymphocytes
Cattle
title_short Local assessment of the immunohistochemical expression of Foxp3+regulatory T lymphocytes in the different pathological forms associated with bovine paratuberculosis
title_full Local assessment of the immunohistochemical expression of Foxp3+regulatory T lymphocytes in the different pathological forms associated with bovine paratuberculosis
title_fullStr Local assessment of the immunohistochemical expression of Foxp3+regulatory T lymphocytes in the different pathological forms associated with bovine paratuberculosis
title_full_unstemmed Local assessment of the immunohistochemical expression of Foxp3+regulatory T lymphocytes in the different pathological forms associated with bovine paratuberculosis
title_sort Local assessment of the immunohistochemical expression of Foxp3+regulatory T lymphocytes in the different pathological forms associated with bovine paratuberculosis
dc.creator.none.fl_str_mv Zapico, David
Espinosa Cerrato, José
Fernández Fernández, Miguel
Criado, M.
Arteche-Villasol, Noive
Pérez Pérez, Valentín
author Zapico, David
author_facet Zapico, David
Espinosa Cerrato, José
Fernández Fernández, Miguel
Criado, M.
Arteche-Villasol, Noive
Pérez Pérez, Valentín
author_role author
author2 Espinosa Cerrato, José
Fernández Fernández, Miguel
Criado, M.
Arteche-Villasol, Noive
Pérez Pérez, Valentín
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Junta de Castilla y León
Universidad de León
Espinosa Cerrato, José [0000-0002-9036-1402]
Arteche Villasol, Noive [0000-0001-5793-9578]
Pérez Pérez, Valentín [0000-0003-0075-1587]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Mycobacterium avium subsp
Paratuberculosis
Foxp3+
Intestinal tissue
Type of lesion
Regulatory T lymphocytes
Cattle
topic Mycobacterium avium subsp
Paratuberculosis
Foxp3+
Intestinal tissue
Type of lesion
Regulatory T lymphocytes
Cattle
description 11 páginas, 3 figuras, 2 tablas.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/279451
url http://hdl.handle.net/10261/279451
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099496-B-I00
info:eu-repo/grantAgreement/MICINN//FJC2019-042422-I
http://dx.doi.org/10.1186/s12917-022-03399-x
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Local assessment of the immunohistochemical expression of Foxp3+regulatory T lymphocytes in the different pathological forms associated with bovine paratuberculosisZapico, DavidEspinosa Cerrato, JoséFernández Fernández, MiguelCriado, M.Arteche-Villasol, NoivePérez Pérez, ValentínMycobacterium avium subspParatuberculosisFoxp3+Intestinal tissueType of lesionRegulatory T lymphocytesCattle11 páginas, 3 figuras, 2 tablas.Background: Mycobacterium avium subsp. paratuberculosis infected animals show a variety of granulomatous lesions, from focal forms with well-demarcated granulomas restricted to the gut-associated lymphoid tissue (GALT), that are seen in the initial phases or latency stages, to a difuse granulomatous enteritis, with abundant (multibacillary) or scant (paucibacillary) bacteria, seen in clinical stages. Factors that determine the response to the infection, respon‑ sible for the occurrence of the diferent types of lesion, are still not fully determined. It has been seen that regulatory T cells (Treg) play an important role in various diseases where they act on the limitation of the immunopathology asso‑ ciated with the immune response. In the case of paratuberculosis (PTB) the role of Treg lymphocytes in the immunity against Map is far away to be completely understood; therefore, several studies addressing this subject have appeared recently. The aim of this work was to assess, by immunohistochemical methods, the presence of Foxp3+ T lympho‑ cytes in intestinal samples with diferent types of lesions seen in cows with PTB. Methods: Intestinal samples of twenty cows showing the diferent pathological forms of PTB were evaluated: uninfected controls (n = 5), focal lesions (n = 5), difuse paucibacillary (n = 5) and difuse multibacillary (n = 5) forms. Foxp3+ lymphocyte distribution was assessed by diferential cell count in intestinal lamina propria (LP), gut-associated lymphoid tissue (GALT) and mesenteric lymph node (MLN). Results: A signifcant increase in the number of Foxp3+ T cells was observed in infected animals with respect to con‑ trol group, regardless of the type of lesion. However, when the diferent categories of lesion were analyzed indepen‑ dently, all individuals with PTB lesions showed an increase in the amount of Foxp3+ T lymphocytes compared to the control group but this increase was only signifcant in cows with focal lesions and, to a lesser extent, in animals with difuse paucibacillary forms. The former showed the highest numbers, signifcantly diferent from those found in cows with difuse lesions, where no diferences were noted between the two forms. No specifc distribution pattern was observed within the granulomatous lesions in any of the groups.This research was funded by grants RTI2018-099496-B-I00 from the Spanish Ministry of Science and Innovation and LE259/P18 from “Junta de Castilla y León”. D. Zapico, M. Criado and N. Arteche-Villasol are supported by a predoc‑ toral contract from the University of León (D. Zapico) or Spanish Ministry of Science and Innovation. J. Espinosa is recipient of a postdoctoral contract of “Juan de la Cierva-Formación (FJC2019-042422-I)” of the Spanish Ministry of Science and Innovation.Peer reviewedBioMed CentralMinisterio de Ciencia e Innovación (España)Junta de Castilla y LeónUniversidad de LeónEspinosa Cerrato, José [0000-0002-9036-1402]Arteche Villasol, Noive [0000-0001-5793-9578]Pérez Pérez, Valentín [0000-0003-0075-1587]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/279451reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099496-B-I00info:eu-repo/grantAgreement/MICINN//FJC2019-042422-Ihttp://dx.doi.org/10.1186/s12917-022-03399-xSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2794512026-05-22T06:33:51Z
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