The CBL-interacting protein kinase CIPK23 regulates HAK5-mediated high-affinity K+ uptake in arabidopsis roots

11 páginas.-- 7 figuras.-- 66 referencias.-- Supplemental Data The following supplemental materials are available. Supplemental Figure S1. Fresh weight of shoots and roots of the Arabidopsis wild type and mutant lines grown in a range of K+ concentrations. Supplemental Figure S2. K+ concentrations o...

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Autores: Ragel, Paula, Ródenas, Reyes, García-Martín, Elena, Andrés, Zaida, Villalta, Irene, Nieves-Cordones, Manuel, Rivero, Rosa M., Martínez, Vicente, Pardo, José M., Quintero, Francisco J., Rubio, Francisco
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/127860
Acceso en línea:http://hdl.handle.net/10261/127860
Access Level:acceso abierto
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spelling The CBL-interacting protein kinase CIPK23 regulates HAK5-mediated high-affinity K+ uptake in arabidopsis rootsRagel, PaulaRódenas, ReyesGarcía-Martín, ElenaAndrés, ZaidaVillalta, IreneNieves-Cordones, ManuelRivero, Rosa M.Martínez, VicentePardo, José M.Quintero, Francisco J.Rubio, Francisco11 páginas.-- 7 figuras.-- 66 referencias.-- Supplemental Data The following supplemental materials are available. Supplemental Figure S1. Fresh weight of shoots and roots of the Arabidopsis wild type and mutant lines grown in a range of K+ concentrations. Supplemental Figure S2. K+ concentrations of shoots of Arabidopsis plants grown in a range of K+ concentrations. Supplemental Table S1. Primers used in this work.Plant growth and development requires efficient acquisition of essential elements. Potassium (K+) is an important macronutrient present in the soil solution at a wide range of concentrations. Regulation of the K+ uptake systems in the roots is essential to secure K+ supply. It has been shown in Arabidopsis (Arabidopsis thaliana) that when the externalK+ concentration is very low (,10 mM), K+ nutrition depends exclusively on the high-affinity K+ transporter5 (HAK5). Low-K+-induced transcriptional activation of the gene encoding HAK5 has been previously reported. Here, we show the posttranscriptional regulation of HAK5 transport activity by phosphorylation. Expression in a heterologous system showed that the Ca2+ sensors calcineurin B-like (CBL1), CBL8, CBL9, and CBL10, together with CBL-interacting protein kinase23 (CIPK23), activated HAK5 in vivo. This activation produced an increase in the affinity and the Vmax of K+ transport. In vitro experiments show that the N terminus of HAK5 is phosphorylated by CIPK23. This supports the idea that phosphorylation of HAK5 induces a conformational change that increases its affinity for K+. Experiments of K+ (Rb+) uptake and growth measurements in low-K+ medium with Arabidopsis single mutants hak5, akt1, and cipk23, double mutants hak5 akt1, hak5 cipk23, and akt1 cipk23, and the triple mutant hak5 akt1 cipk23 confirmed the regulatory role of CIPK23 in planta.This work was supported by the Ministerio de Enonomía y Competitividad, Spain (grant nos. BFU2012–35060 to J.M.P., BIO2012– 36533 to F.J.Q., and AGL2012–33504 to F.R., which were cofinanced by the European Regional Development Fund) and an Formación Profesorado Universitario predoctoral contract from the Ministerio de Educación, Cultura y Deporte (to R.R.).Peer reviewedAmerican Society of Plant BiologistsMinisterio de Economía y Competitividad (España)European CommissionMinisterio de Educación, Cultura y Deporte (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/127860reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1104/pp.15.01401Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1278602026-05-22T06:33:51Z
dc.title.none.fl_str_mv The CBL-interacting protein kinase CIPK23 regulates HAK5-mediated high-affinity K+ uptake in arabidopsis roots
title The CBL-interacting protein kinase CIPK23 regulates HAK5-mediated high-affinity K+ uptake in arabidopsis roots
spellingShingle The CBL-interacting protein kinase CIPK23 regulates HAK5-mediated high-affinity K+ uptake in arabidopsis roots
Ragel, Paula
title_short The CBL-interacting protein kinase CIPK23 regulates HAK5-mediated high-affinity K+ uptake in arabidopsis roots
title_full The CBL-interacting protein kinase CIPK23 regulates HAK5-mediated high-affinity K+ uptake in arabidopsis roots
title_fullStr The CBL-interacting protein kinase CIPK23 regulates HAK5-mediated high-affinity K+ uptake in arabidopsis roots
title_full_unstemmed The CBL-interacting protein kinase CIPK23 regulates HAK5-mediated high-affinity K+ uptake in arabidopsis roots
title_sort The CBL-interacting protein kinase CIPK23 regulates HAK5-mediated high-affinity K+ uptake in arabidopsis roots
dc.creator.none.fl_str_mv Ragel, Paula
Ródenas, Reyes
García-Martín, Elena
Andrés, Zaida
Villalta, Irene
Nieves-Cordones, Manuel
Rivero, Rosa M.
Martínez, Vicente
Pardo, José M.
Quintero, Francisco J.
Rubio, Francisco
author Ragel, Paula
author_facet Ragel, Paula
Ródenas, Reyes
García-Martín, Elena
Andrés, Zaida
Villalta, Irene
Nieves-Cordones, Manuel
Rivero, Rosa M.
Martínez, Vicente
Pardo, José M.
Quintero, Francisco J.
Rubio, Francisco
author_role author
author2 Ródenas, Reyes
García-Martín, Elena
Andrés, Zaida
Villalta, Irene
Nieves-Cordones, Manuel
Rivero, Rosa M.
Martínez, Vicente
Pardo, José M.
Quintero, Francisco J.
Rubio, Francisco
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Ministerio de Educación, Cultura y Deporte (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description 11 páginas.-- 7 figuras.-- 66 referencias.-- Supplemental Data The following supplemental materials are available. Supplemental Figure S1. Fresh weight of shoots and roots of the Arabidopsis wild type and mutant lines grown in a range of K+ concentrations. Supplemental Figure S2. K+ concentrations of shoots of Arabidopsis plants grown in a range of K+ concentrations. Supplemental Table S1. Primers used in this work.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/127860
url http://hdl.handle.net/10261/127860
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1104/pp.15.01401
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society of Plant Biologists
publisher.none.fl_str_mv American Society of Plant Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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