Fluorescence Microscopy of the HIV-1 Envelope

Human immunodeficiency virus (HIV) infection constitutes a major health and social issue worldwide. HIV infects cells by fusing its envelope with the target cell plasma membrane. This process is mediated by the viral Env glycoprotein and depends on the envelope lipid composition. Fluorescent microsc...

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Detalles Bibliográficos
Autores: Carravilla Palomanes, Pablo, Nieva Escandón, José Luis, Eggeling, Christian
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/42877
Acceso en línea:http://hdl.handle.net/10810/42877
Access Level:acceso abierto
Palabra clave:microscopy
HIV
envelope
lipid
membrane
Env
fluorescence
super-resolution
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spelling Fluorescence Microscopy of the HIV-1 EnvelopeCarravilla Palomanes, PabloNieva Escandón, José LuisEggeling, ChristianmicroscopyHIVenvelopelipidmembraneEnvfluorescencesuper-resolutionHuman immunodeficiency virus (HIV) infection constitutes a major health and social issue worldwide. HIV infects cells by fusing its envelope with the target cell plasma membrane. This process is mediated by the viral Env glycoprotein and depends on the envelope lipid composition. Fluorescent microscopy has been employed to investigate the envelope properties, and the processes of viral assembly and fusion, but the application of this technique to the study of HIV is still limited by a number of factors, such as the small size of HIV virions or the difficulty to label the envelope components. Here, we review fluorescence imaging studies of the envelope lipids and proteins, focusing on labelling strategies and model systems.PC was awarded a contract from the Basque Government Postdoctoral Programme (POS_2019_2_0022). JLN acknowledges financial support by the Spanish Government (BIO2015-64421-R (MINECO/AEI/FEDER UE) and RTI2018-095624-B-C21 (MCIU/AEI/FEDER UE) Grants) and the Basque Government (IT1196-19 Grant). CE acknowledges support from the Wolfson Imaging Centre Oxford (Christoffer Lagerholm), the Wolfson Foundation (18272), the Medical Research Council (MC_UU_12010/unit programmes G0902418 and MC_UU_12025), MRC/BBSRC/ESPRC (grant number MR/K01577X/1), the Wellcome Trust (104924/14/Z/14, 100262Z/12/Z, 098274/Z/12/Z and Strategic Award 091911 (107457/Z/15/Z, Advanced Micron Bioimaging Unit)), the Deutsche Forschungsgemeinschaft (Research unit 1905, Jena Excellence Cluster “Balance of the Microverse”, project no. 3162113987 – SFB 1278 (project C05), the Newton-Katip Celebi Institutional Links grant (352333122), and internal University of Oxford funding (EPA Cephalosporin Fund and John Fell Fund).MDPI2020202020202020info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/42877reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MINECO/BIO2015-64421-R/info:eu-repo/grantAgreement/MCIU/RTI2018-095624-B-C21/https://www.mdpi.com/1999-4915/12/3/348info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).oai:addi.ehu.eus:10810/428772026-06-18T09:23:17Z
dc.title.none.fl_str_mv Fluorescence Microscopy of the HIV-1 Envelope
title Fluorescence Microscopy of the HIV-1 Envelope
spellingShingle Fluorescence Microscopy of the HIV-1 Envelope
Carravilla Palomanes, Pablo
microscopy
HIV
envelope
lipid
membrane
Env
fluorescence
super-resolution
title_short Fluorescence Microscopy of the HIV-1 Envelope
title_full Fluorescence Microscopy of the HIV-1 Envelope
title_fullStr Fluorescence Microscopy of the HIV-1 Envelope
title_full_unstemmed Fluorescence Microscopy of the HIV-1 Envelope
title_sort Fluorescence Microscopy of the HIV-1 Envelope
dc.creator.none.fl_str_mv Carravilla Palomanes, Pablo
Nieva Escandón, José Luis
Eggeling, Christian
author Carravilla Palomanes, Pablo
author_facet Carravilla Palomanes, Pablo
Nieva Escandón, José Luis
Eggeling, Christian
author_role author
author2 Nieva Escandón, José Luis
Eggeling, Christian
author2_role author
author
dc.subject.none.fl_str_mv microscopy
HIV
envelope
lipid
membrane
Env
fluorescence
super-resolution
topic microscopy
HIV
envelope
lipid
membrane
Env
fluorescence
super-resolution
description Human immunodeficiency virus (HIV) infection constitutes a major health and social issue worldwide. HIV infects cells by fusing its envelope with the target cell plasma membrane. This process is mediated by the viral Env glycoprotein and depends on the envelope lipid composition. Fluorescent microscopy has been employed to investigate the envelope properties, and the processes of viral assembly and fusion, but the application of this technique to the study of HIV is still limited by a number of factors, such as the small size of HIV virions or the difficulty to label the envelope components. Here, we review fluorescence imaging studies of the envelope lipids and proteins, focusing on labelling strategies and model systems.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/42877
url http://hdl.handle.net/10810/42877
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/BIO2015-64421-R/
info:eu-repo/grantAgreement/MCIU/RTI2018-095624-B-C21/
https://www.mdpi.com/1999-4915/12/3/348
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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