DNA injury induced by 5-aminouracil and caffeine in G2 checkpoints path of higher plant cells

8 p.-5 fig.-3 tab.

Detalles Bibliográficos
Autores: del Campo, Antonio, Bracho, M., Marcano, L., Guíñez, J., Torre, Consuelo de la
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2005
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/103178
Acceso en línea:http://hdl.handle.net/10261/103178
Access Level:acceso abierto
Palabra clave:5-aminouracil
G2 checkpoints
Caffeine
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spelling DNA injury induced by 5-aminouracil and caffeine in G2 checkpoints path of higher plant cellsdel Campo, AntonioBracho, M.Marcano, L.Guíñez, J.Torre, Consuelo de la5-aminouracilG2 checkpointsCaffeine8 p.-5 fig.-3 tab.ABSTRACT: This work evaluated the qualitative and quantitative cellular changes induced by treatment with 5-aminouracil (5-AU) and a combination of 5-AU and caffeine in plant cells in relation to DNA damage, repaired damage, and residual damage. As biological material, Allium cepa L. root tips were used, grown in filtered water, in darkness, with aeration at constant temperature of 25 °C ± 0.5. Cell populations were synchronized using 5 mM caffeine in order to study the effects of 5-AU and caffeine/5-AU combined treatment on the DNA content and their incidence in the entrance to mitosis. The results showed a delay in the G2 period due to induced DNA damage by the 5-AU and caffeine/5-AU combined treatment, shown by aberrant metaphases, anaphases and telophases. The effect of caffeine in the combined treatment was heightened in spite of lengthening the checkpoints route that retains the cells in G2. The existence of G2 checkpoints was shown in the cell population studied, inducing lesions in the DNA, chromosomic aberrations and cellular instability.This work was financed by the Fondo Nacional para la Ciencia y la Tecnología de Venezuela (FONACIT).Peer reviewedUniversidad Nacional de CuyoConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2005info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/103178reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1031782026-05-22T06:33:51Z
dc.title.none.fl_str_mv DNA injury induced by 5-aminouracil and caffeine in G2 checkpoints path of higher plant cells
title DNA injury induced by 5-aminouracil and caffeine in G2 checkpoints path of higher plant cells
spellingShingle DNA injury induced by 5-aminouracil and caffeine in G2 checkpoints path of higher plant cells
del Campo, Antonio
5-aminouracil
G2 checkpoints
Caffeine
title_short DNA injury induced by 5-aminouracil and caffeine in G2 checkpoints path of higher plant cells
title_full DNA injury induced by 5-aminouracil and caffeine in G2 checkpoints path of higher plant cells
title_fullStr DNA injury induced by 5-aminouracil and caffeine in G2 checkpoints path of higher plant cells
title_full_unstemmed DNA injury induced by 5-aminouracil and caffeine in G2 checkpoints path of higher plant cells
title_sort DNA injury induced by 5-aminouracil and caffeine in G2 checkpoints path of higher plant cells
dc.creator.none.fl_str_mv del Campo, Antonio
Bracho, M.
Marcano, L.
Guíñez, J.
Torre, Consuelo de la
author del Campo, Antonio
author_facet del Campo, Antonio
Bracho, M.
Marcano, L.
Guíñez, J.
Torre, Consuelo de la
author_role author
author2 Bracho, M.
Marcano, L.
Guíñez, J.
Torre, Consuelo de la
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv 5-aminouracil
G2 checkpoints
Caffeine
topic 5-aminouracil
G2 checkpoints
Caffeine
description 8 p.-5 fig.-3 tab.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/103178
url http://hdl.handle.net/10261/103178
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidad Nacional de Cuyo
publisher.none.fl_str_mv Universidad Nacional de Cuyo
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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