The tapetal tissue is essential for the maintenance of redox homeostasis during microgametogenesis in tomato
[EN] The tapetum is a specialized layer of cells within the anther, adjacent to the sporogenous tissue. During its short life, it provides nutrients, molecules and materials to the pollen mother cells and microsporocytes, being essential during callose degradation and pollen wall formation. The inte...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/193001 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/193001 |
| Access Level: | acceso abierto |
| Palabra clave: | Solanum lycopersicum Tapetum Pollen ROS TPD1 Anther Male sterility |
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The tapetal tissue is essential for the maintenance of redox homeostasis during microgametogenesis in tomatoSalazar-Sarasúa, BlancaRoque Mesa, Edelin MartaHamza, Rim|||0000-0002-9914-106XCañas Clemente, Luís Antonio|||0000-0003-4177-4777BELTRAN PORTER, JOSE PIOGómez Mena, María Concepción|||0000-0002-1272-3631López-Martín, María JesúsSolanum lycopersicumTapetumPollenROSTPD1AntherMale sterility[EN] The tapetum is a specialized layer of cells within the anther, adjacent to the sporogenous tissue. During its short life, it provides nutrients, molecules and materials to the pollen mother cells and microsporocytes, being essential during callose degradation and pollen wall formation. The interaction between the tapetum and sporogenous cells in Solanum lycopersicum (tomato) plants, despite its importance for breeding purposes, is poorly understood. To investigate this process, gene editing was used to generate loss-of-function mutants that showed the complete and specific absence of tapetal cells. These plants were obtained targeting the previously uncharacterized Solyc03g097530 (SlTPD1) gene, essential for tapetum specification in tomato plants. In the absence of tapetum, sporogenous cells developed and callose deposition was observed. However, sporocytes failed to undergo the process of meiosis and finally degenerated, leading to male sterility. Transcriptomic analysis conducted in mutant anthers lacking tapetum revealed the downregulation of a set of genes related to redox homeostasis. Indeed, mutant anthers showed a reduction in the accumulation of reactive oxygen species (ROS) at early stages and altered activity of ROS-scavenging enzymes. The results obtained highlight the importance of the tapetal tissue in maintaining redox homeostasis during male gametogenesis in tomato plants.This work was supported by grant RTI2018-094280-B-I00 funded by Ministerio de Ciencia e Innovacion (MCIN)/Agencia Estatal de Investigacion (AEI)/10.13039/501100011033 and by Fondo Europeo de Desarrollo Regional (FEDER) `A way of making Europe'. We thank Aureliano Bombarely for his help in the conversion of gene IDs into Solyc identifiers, Diego Orzaez for providing GoldenBraid parts and Maricruz Rochina for expert technical assistance during the project.Blackwell PublishingInstituto Universitario Mixto de Biología Molecular y Celular de PlantasEuropean Regional Development FundMinisterio de Ciencia, Innovación y UniversidadesRepositorio Institucional de la Universitat Politècnica de València Riunet20222022-12-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/193001reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 RTI2018-094280-B-100 Edición de genes de tomate implicados en la gametogénesis masculina: disección del potencial biotecnológico de la androesterilidad en la obtención de frutos sin semillas.open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1930012026-06-13T07:49:27Z |
| dc.title.none.fl_str_mv |
The tapetal tissue is essential for the maintenance of redox homeostasis during microgametogenesis in tomato |
| title |
The tapetal tissue is essential for the maintenance of redox homeostasis during microgametogenesis in tomato |
| spellingShingle |
The tapetal tissue is essential for the maintenance of redox homeostasis during microgametogenesis in tomato Salazar-Sarasúa, Blanca Solanum lycopersicum Tapetum Pollen ROS TPD1 Anther Male sterility |
| title_short |
The tapetal tissue is essential for the maintenance of redox homeostasis during microgametogenesis in tomato |
| title_full |
The tapetal tissue is essential for the maintenance of redox homeostasis during microgametogenesis in tomato |
| title_fullStr |
The tapetal tissue is essential for the maintenance of redox homeostasis during microgametogenesis in tomato |
| title_full_unstemmed |
The tapetal tissue is essential for the maintenance of redox homeostasis during microgametogenesis in tomato |
| title_sort |
The tapetal tissue is essential for the maintenance of redox homeostasis during microgametogenesis in tomato |
| dc.creator.none.fl_str_mv |
Salazar-Sarasúa, Blanca Roque Mesa, Edelin Marta Hamza, Rim|||0000-0002-9914-106X Cañas Clemente, Luís Antonio|||0000-0003-4177-4777 BELTRAN PORTER, JOSE PIO Gómez Mena, María Concepción|||0000-0002-1272-3631 López-Martín, María Jesús |
| author |
Salazar-Sarasúa, Blanca |
| author_facet |
Salazar-Sarasúa, Blanca Roque Mesa, Edelin Marta Hamza, Rim|||0000-0002-9914-106X Cañas Clemente, Luís Antonio|||0000-0003-4177-4777 BELTRAN PORTER, JOSE PIO Gómez Mena, María Concepción|||0000-0002-1272-3631 López-Martín, María Jesús |
| author_role |
author |
| author2 |
Roque Mesa, Edelin Marta Hamza, Rim|||0000-0002-9914-106X Cañas Clemente, Luís Antonio|||0000-0003-4177-4777 BELTRAN PORTER, JOSE PIO Gómez Mena, María Concepción|||0000-0002-1272-3631 López-Martín, María Jesús |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto Universitario Mixto de Biología Molecular y Celular de Plantas European Regional Development Fund Ministerio de Ciencia, Innovación y Universidades Repositorio Institucional de la Universitat Politècnica de València Riunet |
| dc.subject.none.fl_str_mv |
Solanum lycopersicum Tapetum Pollen ROS TPD1 Anther Male sterility |
| topic |
Solanum lycopersicum Tapetum Pollen ROS TPD1 Anther Male sterility |
| description |
[EN] The tapetum is a specialized layer of cells within the anther, adjacent to the sporogenous tissue. During its short life, it provides nutrients, molecules and materials to the pollen mother cells and microsporocytes, being essential during callose degradation and pollen wall formation. The interaction between the tapetum and sporogenous cells in Solanum lycopersicum (tomato) plants, despite its importance for breeding purposes, is poorly understood. To investigate this process, gene editing was used to generate loss-of-function mutants that showed the complete and specific absence of tapetal cells. These plants were obtained targeting the previously uncharacterized Solyc03g097530 (SlTPD1) gene, essential for tapetum specification in tomato plants. In the absence of tapetum, sporogenous cells developed and callose deposition was observed. However, sporocytes failed to undergo the process of meiosis and finally degenerated, leading to male sterility. Transcriptomic analysis conducted in mutant anthers lacking tapetum revealed the downregulation of a set of genes related to redox homeostasis. Indeed, mutant anthers showed a reduction in the accumulation of reactive oxygen species (ROS) at early stages and altered activity of ROS-scavenging enzymes. The results obtained highlight the importance of the tapetal tissue in maintaining redox homeostasis during male gametogenesis in tomato plants. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2022-12-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://riunet.upv.es/handle/10251/193001 |
| url |
https://riunet.upv.es/handle/10251/193001 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 RTI2018-094280-B-100 Edición de genes de tomate implicados en la gametogénesis masculina: disección del potencial biotecnológico de la androesterilidad en la obtención de frutos sin semillas. |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reconocimiento (by) http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reconocimiento (by) http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Blackwell Publishing |
| publisher.none.fl_str_mv |
Blackwell Publishing |
| dc.source.none.fl_str_mv |
reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV) |
| instname_str |
Universitat Politècnica de València (UPV) |
| reponame_str |
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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15,301603 |