PIF transcriptional regulators are required for rhythmic stomatal movements

Stomata govern the gaseous exchange between the leaf and the external atmosphere, and their function is essential for photosynthesis and the global carbon and oxygen cycles. Rhythmic stomata movements in daily dark/light cycles prevent water loss at night and allow CO2 uptake during the day. How the...

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Authors: Rovira Freixa, Arnau, Veciana, Nil, Basté-Miquel, Aina, Quevedo, Martí, Locascio, Antonella, Yenush, Lynne, Toledo-Ortiz, Gabriela, Leivar, Pablo, Monte, Elena
Format: article
Status:Published version
Publication Date:2024
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/372390
Online Access:http://hdl.handle.net/10261/372390
https://api.elsevier.com/content/abstract/scopus_id/85194901264
Access Level:Open access
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dc.title.none.fl_str_mv PIF transcriptional regulators are required for rhythmic stomatal movements
title PIF transcriptional regulators are required for rhythmic stomatal movements
spellingShingle PIF transcriptional regulators are required for rhythmic stomatal movements
Rovira Freixa, Arnau
title_short PIF transcriptional regulators are required for rhythmic stomatal movements
title_full PIF transcriptional regulators are required for rhythmic stomatal movements
title_fullStr PIF transcriptional regulators are required for rhythmic stomatal movements
title_full_unstemmed PIF transcriptional regulators are required for rhythmic stomatal movements
title_sort PIF transcriptional regulators are required for rhythmic stomatal movements
dc.creator.none.fl_str_mv Rovira Freixa, Arnau
Veciana, Nil
Basté-Miquel, Aina
Quevedo, Martí
Locascio, Antonella
Yenush, Lynne
Toledo-Ortiz, Gabriela
Leivar, Pablo
Monte, Elena
author Rovira Freixa, Arnau
author_facet Rovira Freixa, Arnau
Veciana, Nil
Basté-Miquel, Aina
Quevedo, Martí
Locascio, Antonella
Yenush, Lynne
Toledo-Ortiz, Gabriela
Leivar, Pablo
Monte, Elena
author_role author
author2 Veciana, Nil
Basté-Miquel, Aina
Quevedo, Martí
Locascio, Antonella
Yenush, Lynne
Toledo-Ortiz, Gabriela
Leivar, Pablo
Monte, Elena
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Generalitat de Catalunya
Daiwa Anglo-Japanese Foundation
Royal Society (UK)
Ministerio de Economía y Competitividad (España)
Rovira Freixa, Arnau [0000-0002-8583-1828]
Veciana, Nil [0000-0002-1761-6616]
Quevedo, Martí [0000-0001-7747-7710]
Locascio, Antonella [0000-0001-8026-1937]
Yenush, Lynne [0000-0001-8589-7002]
Leivar, Pablo [0000-0003-4878-3684]
Monte, Elena [0000-0002-7340-9355]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Stomata govern the gaseous exchange between the leaf and the external atmosphere, and their function is essential for photosynthesis and the global carbon and oxygen cycles. Rhythmic stomata movements in daily dark/light cycles prevent water loss at night and allow CO2 uptake during the day. How the actors involved are transcriptionally regulated and how this might contribute to rhythmicity is largely unknown. Here, we show that morning stomata opening depends on the previous night period. The transcription factors PHYTOCHROME-INTERACTING FACTORS (PIFs) accumulate at the end of the night and directly induce the guard cell-specific K+ channel KAT1. Remarkably, PIFs and KAT1 are required for blue light-induced stomata opening. Together, our data establish a molecular framework for daily rhythmic stomatal movements under well-watered conditions, whereby PIFs are required for accumulation of KAT1 at night, which upon activation by blue light in the morning leads to the K+ intake driving stomata opening.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
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dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/372390
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url http://hdl.handle.net/10261/372390
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Nature Communications
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI http://dx.doi.org/10.1038/s41467-024-48669-4
http://dx.doi.org/10.1038/s41467-024-48669-4
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spelling PIF transcriptional regulators are required for rhythmic stomatal movementsRovira Freixa, ArnauVeciana, NilBasté-Miquel, AinaQuevedo, MartíLocascio, AntonellaYenush, LynneToledo-Ortiz, GabrielaLeivar, PabloMonte, ElenaStomata govern the gaseous exchange between the leaf and the external atmosphere, and their function is essential for photosynthesis and the global carbon and oxygen cycles. Rhythmic stomata movements in daily dark/light cycles prevent water loss at night and allow CO2 uptake during the day. How the actors involved are transcriptionally regulated and how this might contribute to rhythmicity is largely unknown. Here, we show that morning stomata opening depends on the previous night period. The transcription factors PHYTOCHROME-INTERACTING FACTORS (PIFs) accumulate at the end of the night and directly induce the guard cell-specific K+ channel KAT1. Remarkably, PIFs and KAT1 are required for blue light-induced stomata opening. Together, our data establish a molecular framework for daily rhythmic stomatal movements under well-watered conditions, whereby PIFs are required for accumulation of KAT1 at night, which upon activation by blue light in the morning leads to the K+ intake driving stomata opening.We are grateful to ABRC/NASC for kat1 and aba2 seeds, and to Luis Oñate-Sánchez for Y1H materials, protocols, and advice. This work was supported by grants from FEDER/Ministerio de Ciencia, Innovación y Universidades – Agencia Estatal de Investigación (Project References BIO2015-68460-P, PGC2018-099987-B-I00, and PID2021-122288NB-I00 to E.M.; PID2019-10454GB-I00 to L.Y.), from the CERCA Programme/Generalitat de Catalunya (Project Reference 2017SGR-718) to E.M, and from the Daiwa Anglo Japanese Foundation (1019/13721) and the Royal Society (IEC\R1\180047) to G.T-O. We acknowledge financial support from the Spanish Ministry of Economy and Competitiveness, through the ‘Severo Ochoa Programme for Centres of Excellence in R&D’ 2016–2019 (SEV-2015-0533) and CEX2019-000902-S funded by MCIN/AEI/10.13039/501100011033. A.B.-M. was supported by the predoctoral program AGAUR-FI ajuts (2023 FI-1 00910) Joan Oró of the Secretariat of Universities and Research of the Department of Research and Universities of the Generalitat of Catalonia and the European Social Plus Fund, and M.Q. received postdoctoral funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 945043.With funding from the Spanish goverment through the "Severo Ochoa Centre of Excellence" accreditation (SEV-2015-0533 and CEX2019-000902-S)Peer reviewedSpringer NatureEuropean CommissionMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Generalitat de CatalunyaDaiwa Anglo-Japanese FoundationRoyal Society (UK)Ministerio de Economía y Competitividad (España)Rovira Freixa, Arnau [0000-0002-8583-1828]Veciana, Nil [0000-0002-1761-6616]Quevedo, Martí [0000-0001-7747-7710]Locascio, Antonella [0000-0001-8026-1937]Yenush, Lynne [0000-0001-8589-7002]Leivar, Pablo [0000-0003-4878-3684]Monte, Elena [0000-0002-7340-9355]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/372390https://api.elsevier.com/content/abstract/scopus_id/85194901264reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//BIO2015-68460-Pinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-099987-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122288NB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104544GB-I00info:eu-repo/grantAgreement/MINECO//SEV-2015-0533info:eu-repo/grantAgreement/AEI//CEX2019-000902-SNature CommunicationsThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI http://dx.doi.org/10.1038/s41467-024-48669-4http://dx.doi.org/10.1038/s41467-024-48669-4Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3723902026-05-22T06:33:51Z
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