Establishment and Validation of a New Co-Culture for the Evaluation of the Permeability through the Blood–Brain Barrier in Patients with Glioblastoma

Currently, the mechanisms involved in drug access to the central nervous system (CNS) are not completely elucidated, and research efforts to understand the behaviour of the therapeutic agents to access the blood–brain barrier continue with the utmost importance. The aim of this work was the creation...

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Detalhes bibliográficos
Autores: Sánchez-Dengra, Bárbara, García-Montoya, Elena, González Álvarez, Isabel, Bermejo, Marival, González Álvarez, Marta
Formato: artículo
Fecha de publicación:2023
País:España
Recursos:Universidad Miguel Hernández de Elche
Repositorio:REDIUMH. Depósito Digital de la UMH
OAI Identifier:oai:dspace.umh.es:11000/34645
Acesso em linha:https://hdl.handle.net/11000/34645
Access Level:acceso abierto
Palavra-chave:blood–brain barrier (BBB)
cell culture
glioblastoma
permeability
in vitro–in vivo correlations (IVIVC)
transepithelial electrical resistance (TEER)
CDU::6 - Ciencias aplicadas::62 - Ingeniería. Tecnología
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spelling Establishment and Validation of a New Co-Culture for the Evaluation of the Permeability through the Blood–Brain Barrier in Patients with GlioblastomaSánchez-Dengra, BárbaraGarcía-Montoya, ElenaGonzález Álvarez, IsabelBermejo, MarivalGonzález Álvarez, Martablood–brain barrier (BBB)cell cultureglioblastomapermeabilityin vitro–in vivo correlations (IVIVC)transepithelial electrical resistance (TEER)CDU::6 - Ciencias aplicadas::62 - Ingeniería. TecnologíaCurrently, the mechanisms involved in drug access to the central nervous system (CNS) are not completely elucidated, and research efforts to understand the behaviour of the therapeutic agents to access the blood–brain barrier continue with the utmost importance. The aim of this work was the creation and validation of a new in vitro model capable of predicting the in vivo permeability across the blood–brain barrier in the presence of glioblastoma. The selected in vitro method was a cell co-culture model of epithelial cell lines (MDCK and MDCK-MDR1) with a glioblastoma cell line (U87-MG). Several drugs were tested (letrozole, gemcitabine, methotrexate and ganciclovir). Comparison of the proposed in vitro model, MDCK and MDCK-MDR1 co-cultured with U87-MG, and in vivo studies showed a great predictability for each cell line, with R2 values of 0.8917 and 0.8296, respectively. Therefore, both cells lines (MDCK and MDCK-MDR1) are valid for predicting the access of drugs to the CNS in the presence of glioblastoma.MDPIDepartamentos de la UMH::Ingeniería202520252023info:eu-repo/semantics/articleapplication/pdf12application/pdfhttps://hdl.handle.net/11000/34645reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheIngléshttps://doi.org/10.3390/pharmaceutics15051431info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:dspace.umh.es:11000/346452026-05-27T13:36:21Z
dc.title.none.fl_str_mv Establishment and Validation of a New Co-Culture for the Evaluation of the Permeability through the Blood–Brain Barrier in Patients with Glioblastoma
title Establishment and Validation of a New Co-Culture for the Evaluation of the Permeability through the Blood–Brain Barrier in Patients with Glioblastoma
spellingShingle Establishment and Validation of a New Co-Culture for the Evaluation of the Permeability through the Blood–Brain Barrier in Patients with Glioblastoma
Sánchez-Dengra, Bárbara
blood–brain barrier (BBB)
cell culture
glioblastoma
permeability
in vitro–in vivo correlations (IVIVC)
transepithelial electrical resistance (TEER)
CDU::6 - Ciencias aplicadas::62 - Ingeniería. Tecnología
title_short Establishment and Validation of a New Co-Culture for the Evaluation of the Permeability through the Blood–Brain Barrier in Patients with Glioblastoma
title_full Establishment and Validation of a New Co-Culture for the Evaluation of the Permeability through the Blood–Brain Barrier in Patients with Glioblastoma
title_fullStr Establishment and Validation of a New Co-Culture for the Evaluation of the Permeability through the Blood–Brain Barrier in Patients with Glioblastoma
title_full_unstemmed Establishment and Validation of a New Co-Culture for the Evaluation of the Permeability through the Blood–Brain Barrier in Patients with Glioblastoma
title_sort Establishment and Validation of a New Co-Culture for the Evaluation of the Permeability through the Blood–Brain Barrier in Patients with Glioblastoma
dc.creator.none.fl_str_mv Sánchez-Dengra, Bárbara
García-Montoya, Elena
González Álvarez, Isabel
Bermejo, Marival
González Álvarez, Marta
author Sánchez-Dengra, Bárbara
author_facet Sánchez-Dengra, Bárbara
García-Montoya, Elena
González Álvarez, Isabel
Bermejo, Marival
González Álvarez, Marta
author_role author
author2 García-Montoya, Elena
González Álvarez, Isabel
Bermejo, Marival
González Álvarez, Marta
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Departamentos de la UMH::Ingeniería
dc.subject.none.fl_str_mv blood–brain barrier (BBB)
cell culture
glioblastoma
permeability
in vitro–in vivo correlations (IVIVC)
transepithelial electrical resistance (TEER)
CDU::6 - Ciencias aplicadas::62 - Ingeniería. Tecnología
topic blood–brain barrier (BBB)
cell culture
glioblastoma
permeability
in vitro–in vivo correlations (IVIVC)
transepithelial electrical resistance (TEER)
CDU::6 - Ciencias aplicadas::62 - Ingeniería. Tecnología
description Currently, the mechanisms involved in drug access to the central nervous system (CNS) are not completely elucidated, and research efforts to understand the behaviour of the therapeutic agents to access the blood–brain barrier continue with the utmost importance. The aim of this work was the creation and validation of a new in vitro model capable of predicting the in vivo permeability across the blood–brain barrier in the presence of glioblastoma. The selected in vitro method was a cell co-culture model of epithelial cell lines (MDCK and MDCK-MDR1) with a glioblastoma cell line (U87-MG). Several drugs were tested (letrozole, gemcitabine, methotrexate and ganciclovir). Comparison of the proposed in vitro model, MDCK and MDCK-MDR1 co-cultured with U87-MG, and in vivo studies showed a great predictability for each cell line, with R2 values of 0.8917 and 0.8296, respectively. Therefore, both cells lines (MDCK and MDCK-MDR1) are valid for predicting the access of drugs to the CNS in the presence of glioblastoma.
publishDate 2023
dc.date.none.fl_str_mv 2023
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/11000/34645
url https://hdl.handle.net/11000/34645
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/pharmaceutics15051431
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
12
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:REDIUMH. Depósito Digital de la UMH
instname:Universidad Miguel Hernández de Elche
instname_str Universidad Miguel Hernández de Elche
reponame_str REDIUMH. Depósito Digital de la UMH
collection REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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