Enzyme controlled transient phospholipid vesicles for regulated cargo release

Metabolism in biological systems involves the continuous formation and breakdown of chemical and structural components, driven by chemical energy. In specific, metabolic processes on cellular membranes result in in situ formation and degradation of the constituent phospholipid molecules, by consumin...

Descripción completa

Detalles Bibliográficos
Autores: Venugopal, Akhil, Ghosh, Subhadip, Calò, Annalisa, Tuveri, Gian Marco, Battaglia, Giuseppe, Kumar, Mohit
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/222125
Acceso en línea:https://hdl.handle.net/2445/222125
Access Level:acceso abierto
Palabra clave:Nanomedicina
Membranes (Biologia)
Fosfolípids
Nanomedicine
Membranes (Biology)
Phospholipids
id ES_6d4d4053800a1a63abcfb176ada6c1ad
oai_identifier_str oai:recercat.cat:2445/222125
network_acronym_str ES
network_name_str España
repository_id_str
spelling Enzyme controlled transient phospholipid vesicles for regulated cargo releaseVenugopal, AkhilGhosh, SubhadipCalò, AnnalisaTuveri, Gian MarcoBattaglia, GiuseppeKumar, MohitNanomedicinaMembranes (Biologia)FosfolípidsNanomedicineMembranes (Biology)PhospholipidsMetabolism in biological systems involves the continuous formation and breakdown of chemical and structural components, driven by chemical energy. In specific, metabolic processes on cellular membranes result in in situ formation and degradation of the constituent phospholipid molecules, by consuming fuel, to dynamically regulate the properties. Synthetic analogs of such chemically fueled phospholipid vesicles have been challenging. Here we report a bio-inspired approach for the in situ formation of phospholipids, from water soluble precursors, and their fuel driven self-assembly into vesicles. We show that the kinetic competition between anabolic and catabolic-like reactions leads to the formation and enzymatic degradation of the double-tailed, vesicle-forming phospholipid. Spectroscopic and microscopic analysis demonstrate the formation of transient vesicles whose lifetime can be easily tuned from minutes to hours. Importantly, our design results in the formation of uniform sized (65 nm) vesicles simply by mixing the precursors, thus avoiding the traditional complex methods. Finally, our sub-100 nm vesicles are of the right size for application in drug delivery. We have demonstrated that the release kinetics of the incorporated cargo molecules can be dynamically regulated for potential applications in adaptive nanomedicine.Wiley-VCH2025202520252025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion7 p.application/pdfhttps://hdl.handle.net/2445/222125Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1002/anie.202500824Angewandte Chemie-International Edition, 2025, vol. 64, num.20https://doi.org/10.1002/anie.202500824cc-by-nc (c) Venugopal et al., 2025http://creativecommons.org/licenses/by-nc/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2221252026-05-29T05:05:01Z
dc.title.none.fl_str_mv Enzyme controlled transient phospholipid vesicles for regulated cargo release
title Enzyme controlled transient phospholipid vesicles for regulated cargo release
spellingShingle Enzyme controlled transient phospholipid vesicles for regulated cargo release
Venugopal, Akhil
Nanomedicina
Membranes (Biologia)
Fosfolípids
Nanomedicine
Membranes (Biology)
Phospholipids
title_short Enzyme controlled transient phospholipid vesicles for regulated cargo release
title_full Enzyme controlled transient phospholipid vesicles for regulated cargo release
title_fullStr Enzyme controlled transient phospholipid vesicles for regulated cargo release
title_full_unstemmed Enzyme controlled transient phospholipid vesicles for regulated cargo release
title_sort Enzyme controlled transient phospholipid vesicles for regulated cargo release
dc.creator.none.fl_str_mv Venugopal, Akhil
Ghosh, Subhadip
Calò, Annalisa
Tuveri, Gian Marco
Battaglia, Giuseppe
Kumar, Mohit
author Venugopal, Akhil
author_facet Venugopal, Akhil
Ghosh, Subhadip
Calò, Annalisa
Tuveri, Gian Marco
Battaglia, Giuseppe
Kumar, Mohit
author_role author
author2 Ghosh, Subhadip
Calò, Annalisa
Tuveri, Gian Marco
Battaglia, Giuseppe
Kumar, Mohit
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Nanomedicina
Membranes (Biologia)
Fosfolípids
Nanomedicine
Membranes (Biology)
Phospholipids
topic Nanomedicina
Membranes (Biologia)
Fosfolípids
Nanomedicine
Membranes (Biology)
Phospholipids
description Metabolism in biological systems involves the continuous formation and breakdown of chemical and structural components, driven by chemical energy. In specific, metabolic processes on cellular membranes result in in situ formation and degradation of the constituent phospholipid molecules, by consuming fuel, to dynamically regulate the properties. Synthetic analogs of such chemically fueled phospholipid vesicles have been challenging. Here we report a bio-inspired approach for the in situ formation of phospholipids, from water soluble precursors, and their fuel driven self-assembly into vesicles. We show that the kinetic competition between anabolic and catabolic-like reactions leads to the formation and enzymatic degradation of the double-tailed, vesicle-forming phospholipid. Spectroscopic and microscopic analysis demonstrate the formation of transient vesicles whose lifetime can be easily tuned from minutes to hours. Importantly, our design results in the formation of uniform sized (65 nm) vesicles simply by mixing the precursors, thus avoiding the traditional complex methods. Finally, our sub-100 nm vesicles are of the right size for application in drug delivery. We have demonstrated that the release kinetics of the incorporated cargo molecules can be dynamically regulated for potential applications in adaptive nanomedicine.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/222125
url https://hdl.handle.net/2445/222125
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1002/anie.202500824
Angewandte Chemie-International Edition, 2025, vol. 64, num.20
https://doi.org/10.1002/anie.202500824
dc.rights.none.fl_str_mv cc-by-nc (c) Venugopal et al., 2025
http://creativecommons.org/licenses/by-nc/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc (c) Venugopal et al., 2025
http://creativecommons.org/licenses/by-nc/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7 p.
application/pdf
dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410342072745984
score 15,81155

Similares en LA Referencia