Lupin-based high-protein extrudates: Physicochemical, nutritional and techno-functional properties

[EN] Lupin is an underutilized legume with high protein content (30-40 %) and strong potential for sustainable cultivation, but its use in the food industry is limited by the presence of anti-nutritional (saponins, alkaloids, polyphenols) and anti-technological factors, as well as suboptimal techno-...

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Bibliographic Details
Authors: Navarro-Vozmediano, Paola|||0000-0003-1909-6121, Garcia-Perez, J.V.|||0000-0002-9993-4246, Quiles Chuliá, Mª Desamparados|||0000-0003-4991-6629, Benedito Fort, José Javier|||0000-0003-0163-4593, Bou, Ricard, Jorba-Martín, Rosa
Format: article
Publication Date:2026
Country:España
Institution:Universitat Politècnica de València (UPV)
Repository:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Language:English
OAI Identifier:oai:dnet:riunet______::0d8cd5ee07352f2fa18e07424c09f08d
Online Access:https://riunet.upv.es/handle/10251/235933
Access Level:Open access
Keyword:Extrusion
Lupin protein
Anti-nutritional factors
Anti-technological factors
Techno-functional properties
Texture
Description
Summary:[EN] Lupin is an underutilized legume with high protein content (30-40 %) and strong potential for sustainable cultivation, but its use in the food industry is limited by the presence of anti-nutritional (saponins, alkaloids, polyphenols) and anti-technological factors, as well as suboptimal techno-functional properties. Low-moisture extrusion (LME) offers a promising strategy to overcome these limitations while producing high-protein texturized ingredients. In this study, five formulations combining lupin flour with laboratory or commercial lupin protein isolates were subjected to LME to evaluate effects on anti-nutritional compounds, techno-functional properties, and textural attributes. Extrusion reduced polyphenols (avg. 42 %), antioxidant activity (avg. 17 %) and saponins (avg. 47 %), while alkaloids remained stable. Water and fat absorption capacities were largely maintained (avg. 1.26 g water/g dm and 1.66 g oil/g dm, respectively), compared to powder formulations (avg. 1.31 g water/g dm and 2.25 g oil/g dm, respectively). Microstructural observations, along with bulk density (avg. 1.09 g/cm3) and texture analysis (average hardness of 346 N) confirmed the formation of compact highprotein extrudates (up to 88.6 % protein) and showed the effects of protein incorporation on matrix organization and textural properties, supporting their suitability as functional ingredients. Overall, this work demonstrates that LME can enhance the properties of lupin flour and isolates, producing high-protein extrudates with improved nutritional, functional, and structural qualities.