Response of rat cerebral somatostatinergic system to a high ammonia diet
It has been reported that ingestion of an ammonium-containing diet produces hyperammonemia without encephalopathy, thus permitting the study of the specific effects of ammonia toxicity. The present study investigated the rat cerebral somatostatinergic system using this experimental model of hyperamm...
| Autores: | , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 1996 |
| País: | España |
| Institución: | Universidad de Alcalá (UAH) |
| Repositorio: | e_Buah Biblioteca Digital Universidad de Alcalá |
| Idioma: | inglés |
| OAI Identifier: | oai:ebuah.uah.es:10017/2274 |
| Acceso en línea: | http://hdl.handle.net/10017/2274 https://dx.doi.org/10.1016/0197-0186(96)00021-6 |
| Access Level: | acceso abierto |
| Palabra clave: | Encephalopathy Wistar rats Ammonium acetate Somatostatin Frontoparietal cortex Hippocampus Bioquímica Ciencia Biochemistry Science |
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Response of rat cerebral somatostatinergic system to a high ammonia dietBoyano Adánez, María del Carmen|||0000-0003-2799-6613Bodega Magro, Guillermo|||0000-0003-1469-3242Barrios Sabador, VicenteEncephalopathyWistar ratsAmmonium acetateSomatostatinFrontoparietal cortexHippocampusSomatostatinBioquímicaCienciaBiochemistryScienceIt has been reported that ingestion of an ammonium-containing diet produces hyperammonemia without encephalopathy, thus permitting the study of the specific effects of ammonia toxicity. The present study investigated the rat cerebral somatostatinergic system using this experimental model of hyperammonemia. Wistar rats were fed a high ammonia diet prepared by mixing a standard diet with ammonium acetate (20% w/w); in addition, 5 mM of ammonium acetate was added to their water supply. Control rats were fed with a standard diet. The animals were sacrificed at 3, 7 and 15 days of ammonia ingestion. Ammonia levels in blood had increased ¿3-fold at 7 days of ammonia ingestion. These changes were associated with a significant decrease in the specific binding of somatostatin (SS) to putative receptors sites in the frontoparietal cortex and hippocampus at 7 and 15 days after starting the high ammonia diet. Scatchard analysis shows that the decrease in SS binding resulted from a decrease in the number of available SS receptors rather than a change in receptor affinity. No changes in the somatostatin-like immunoreactivity content (SSLI) were detected in either brain area at the three study times. These results suggest that hyperammonemia alone can affect the rat brain somatostatinergic system. However, the animal model of hyperammonemia used here is insufficient to produce encephalopathy despite the significant increase in serum ammonia.Universidad de AlcaláInstituto de Salud Carlos IIIMinisterio de Educación y CulturaPergamon19961996-01-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/2274https://dx.doi.org/10.1016/0197-0186(96)00021-6reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengUAH Not available 001%2F96 REGULACION DEL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA POR EL NEUROPEPTIDO Y (NPY) EN EL CEREBRO DE LA RATAUAH Not available 008%2F96 MODULACION DEL SISTEMA SOMATOSTATINÉRGICO CEREBRAL DE LA RATA POR EL ÓXIDO NÍTRICOMinisterio de Educación y Ciencia Not available PM95-0041 EFECTO DEL OXIDO NITRICO SOBRE EL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA EN EL CEREBRO HUMANO Y DE RATANot available Not available Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/22742026-06-18T11:13:07Z |
| dc.title.none.fl_str_mv |
Response of rat cerebral somatostatinergic system to a high ammonia diet |
| title |
Response of rat cerebral somatostatinergic system to a high ammonia diet |
| spellingShingle |
Response of rat cerebral somatostatinergic system to a high ammonia diet Boyano Adánez, María del Carmen|||0000-0003-2799-6613 Encephalopathy Wistar rats Ammonium acetate Somatostatin Frontoparietal cortex Hippocampus Somatostatin Bioquímica Ciencia Biochemistry Science |
| title_short |
Response of rat cerebral somatostatinergic system to a high ammonia diet |
| title_full |
Response of rat cerebral somatostatinergic system to a high ammonia diet |
| title_fullStr |
Response of rat cerebral somatostatinergic system to a high ammonia diet |
| title_full_unstemmed |
Response of rat cerebral somatostatinergic system to a high ammonia diet |
| title_sort |
Response of rat cerebral somatostatinergic system to a high ammonia diet |
| dc.creator.none.fl_str_mv |
Boyano Adánez, María del Carmen|||0000-0003-2799-6613 Bodega Magro, Guillermo|||0000-0003-1469-3242 Barrios Sabador, Vicente |
| author |
Boyano Adánez, María del Carmen|||0000-0003-2799-6613 |
| author_facet |
Boyano Adánez, María del Carmen|||0000-0003-2799-6613 Bodega Magro, Guillermo|||0000-0003-1469-3242 Barrios Sabador, Vicente |
| author_role |
author |
| author2 |
Bodega Magro, Guillermo|||0000-0003-1469-3242 Barrios Sabador, Vicente |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
Encephalopathy Wistar rats Ammonium acetate Somatostatin Frontoparietal cortex Hippocampus Somatostatin Bioquímica Ciencia Biochemistry Science |
| topic |
Encephalopathy Wistar rats Ammonium acetate Somatostatin Frontoparietal cortex Hippocampus Somatostatin Bioquímica Ciencia Biochemistry Science |
| description |
It has been reported that ingestion of an ammonium-containing diet produces hyperammonemia without encephalopathy, thus permitting the study of the specific effects of ammonia toxicity. The present study investigated the rat cerebral somatostatinergic system using this experimental model of hyperammonemia. Wistar rats were fed a high ammonia diet prepared by mixing a standard diet with ammonium acetate (20% w/w); in addition, 5 mM of ammonium acetate was added to their water supply. Control rats were fed with a standard diet. The animals were sacrificed at 3, 7 and 15 days of ammonia ingestion. Ammonia levels in blood had increased ¿3-fold at 7 days of ammonia ingestion. These changes were associated with a significant decrease in the specific binding of somatostatin (SS) to putative receptors sites in the frontoparietal cortex and hippocampus at 7 and 15 days after starting the high ammonia diet. Scatchard analysis shows that the decrease in SS binding resulted from a decrease in the number of available SS receptors rather than a change in receptor affinity. No changes in the somatostatin-like immunoreactivity content (SSLI) were detected in either brain area at the three study times. These results suggest that hyperammonemia alone can affect the rat brain somatostatinergic system. However, the animal model of hyperammonemia used here is insufficient to produce encephalopathy despite the significant increase in serum ammonia. |
| publishDate |
1996 |
| dc.date.none.fl_str_mv |
1996 1996-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 NA http://purl.org/coar/version/c_be7fb7dd8ff6fe43 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10017/2274 https://dx.doi.org/10.1016/0197-0186(96)00021-6 |
| url |
http://hdl.handle.net/10017/2274 https://dx.doi.org/10.1016/0197-0186(96)00021-6 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
UAH Not available 001%2F96 REGULACION DEL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA POR EL NEUROPEPTIDO Y (NPY) EN EL CEREBRO DE LA RATA UAH Not available 008%2F96 MODULACION DEL SISTEMA SOMATOSTATINÉRGICO CEREBRAL DE LA RATA POR EL ÓXIDO NÍTRICO Ministerio de Educación y Ciencia Not available PM95-0041 EFECTO DEL OXIDO NITRICO SOBRE EL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA EN EL CEREBRO HUMANO Y DE RATA Not available Not available Not available |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Pergamon |
| publisher.none.fl_str_mv |
Pergamon |
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reponame:e_Buah Biblioteca Digital Universidad de Alcalá instname:Universidad de Alcalá (UAH) |
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Universidad de Alcalá (UAH) |
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e_Buah Biblioteca Digital Universidad de Alcalá |
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e_Buah Biblioteca Digital Universidad de Alcalá |
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1869410307234856960 |
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15,300724 |