Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture

The aim of this work was to study the effect of Se(+VI) on viability, cell morphology, and selenomethionine accumulation of the green alga Chlorella sorokiniana grown in batch cultures. Culture exposed to sublethal Se concentrations of 40 mg⋅L−1 (212 μ M) decreased growth rates for about 25% compare...

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Authors: Gojkovic, Živan, Vílchez Lobato, Carlos, Torronteras Santiago, Rafael, Vigara Fernández, Javier, Gómez Jacinto, Verónica, Janzer, Nora, Gómez Ariza, José Luis, Márová, Ivana, Garbayo Nores, Inés
Format: article
Publication Date:2014
Country:España
Institution:Universidad de Huelva (UHU)
Repository:Arias Montano. Repositorio Institucional de la Universidad de Huelva
Language:English
OAI Identifier:oai:ariasmontano.uhu.es:10272/8383
Online Access:http://hdl.handle.net/10272/8383
Access Level:Open access
Keyword:Selenate
Chlorella sorokiniana
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spelling Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch CultureGojkovic, ŽivanVílchez Lobato, CarlosTorronteras Santiago, RafaelVigara Fernández, JavierGómez Jacinto, VerónicaJanzer, NoraGómez Ariza, José LuisMárová, IvanaGarbayo Nores, InésSelenateChlorella sorokinianaThe aim of this work was to study the effect of Se(+VI) on viability, cell morphology, and selenomethionine accumulation of the green alga Chlorella sorokiniana grown in batch cultures. Culture exposed to sublethal Se concentrations of 40 mg⋅L−1 (212 μ M) decreased growth rates for about 25% compared to control. A selenate EC50 value of 45mg⋅L−1 (238.2 μ M) was determined. Results showed that chlorophyll and carotenoids contents were not affected by Se exposure, while oxygen evolution decreased by half. Ultrastructural studies revealed granular stroma, fingerprint-like appearance of thylakoids which did not compromise cell activity. Unlike control cultures, SDS PAGE electrophoresis of crude extracts from selenate-exposed cell cultures revealed appearance of a protein band identified as 53 kDa Rubisco large subunit of Chlorella sorokiniana, suggesting that selenate affects expression of the corresponding chloroplast gene as this subunit is encoded in the chloroplast DNA. Results revealed that the microalga was able to accumulate up to 140mg⋅kg−1 of SeMet in 120h of cultivation.This paper shows that Chlorella sorokiniana biomass can be enriched in the high value aminoacid SeMet in batch cultures, while keeping photochemical viability and carbon dioxide fixation activity intact, if exposed to suitable sublethal concentrations of Se.Hindawi Publishing Corporation20142014-01-0120142014-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10272/8383reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelvainstname:Universidad de Huelva (UHU)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:ariasmontano.uhu.es:10272/83832026-06-02T14:58:11Z
dc.title.none.fl_str_mv Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture
title Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture
spellingShingle Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture
Gojkovic, Živan
Selenate
Chlorella sorokiniana
title_short Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture
title_full Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture
title_fullStr Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture
title_full_unstemmed Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture
title_sort Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture
dc.creator.none.fl_str_mv Gojkovic, Živan
Vílchez Lobato, Carlos
Torronteras Santiago, Rafael
Vigara Fernández, Javier
Gómez Jacinto, Verónica
Janzer, Nora
Gómez Ariza, José Luis
Márová, Ivana
Garbayo Nores, Inés
author Gojkovic, Živan
author_facet Gojkovic, Živan
Vílchez Lobato, Carlos
Torronteras Santiago, Rafael
Vigara Fernández, Javier
Gómez Jacinto, Verónica
Janzer, Nora
Gómez Ariza, José Luis
Márová, Ivana
Garbayo Nores, Inés
author_role author
author2 Vílchez Lobato, Carlos
Torronteras Santiago, Rafael
Vigara Fernández, Javier
Gómez Jacinto, Verónica
Janzer, Nora
Gómez Ariza, José Luis
Márová, Ivana
Garbayo Nores, Inés
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Selenate
Chlorella sorokiniana
topic Selenate
Chlorella sorokiniana
description The aim of this work was to study the effect of Se(+VI) on viability, cell morphology, and selenomethionine accumulation of the green alga Chlorella sorokiniana grown in batch cultures. Culture exposed to sublethal Se concentrations of 40 mg⋅L−1 (212 μ M) decreased growth rates for about 25% compared to control. A selenate EC50 value of 45mg⋅L−1 (238.2 μ M) was determined. Results showed that chlorophyll and carotenoids contents were not affected by Se exposure, while oxygen evolution decreased by half. Ultrastructural studies revealed granular stroma, fingerprint-like appearance of thylakoids which did not compromise cell activity. Unlike control cultures, SDS PAGE electrophoresis of crude extracts from selenate-exposed cell cultures revealed appearance of a protein band identified as 53 kDa Rubisco large subunit of Chlorella sorokiniana, suggesting that selenate affects expression of the corresponding chloroplast gene as this subunit is encoded in the chloroplast DNA. Results revealed that the microalga was able to accumulate up to 140mg⋅kg−1 of SeMet in 120h of cultivation.This paper shows that Chlorella sorokiniana biomass can be enriched in the high value aminoacid SeMet in batch cultures, while keeping photochemical viability and carbon dioxide fixation activity intact, if exposed to suitable sublethal concentrations of Se.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01
2014
2014-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10272/8383
url http://hdl.handle.net/10272/8383
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelva
instname:Universidad de Huelva (UHU)
instname_str Universidad de Huelva (UHU)
reponame_str Arias Montano. Repositorio Institucional de la Universidad de Huelva
collection Arias Montano. Repositorio Institucional de la Universidad de Huelva
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410304500170752
score 15,811543

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