Synthetic RNAs mimicking structural domains in the foot-and-mouth disease virus genome elicit a broad innate immune response in porcine cells triggered by RIG-I and TLR activation

The innate immune system is the first line of defense against viral infections. Exploiting innate responses for antiviral, therapeutic and vaccine adjuvation strategies is being extensively explored. We have previously described, the ability of small in vitro RNA transcripts, mimicking the sequence...

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Detalles Bibliográficos
Autores: Borrego, Belén, Rodríguez Pulido, Miguel Ramón, Revilla Calvo, Concepción, Álvarez, Belén, Sobrino Castelló, Francisco, Domínguez, Javier, Sáiz, Margarita
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/149836
Acceso en línea:http://hdl.handle.net/10261/149836
Access Level:acceso abierto
Palabra clave:Viral sensors
Antivirals
Non-coding RNA
Innate immunity
Foot-and-mouth
Disease virus
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spelling Synthetic RNAs mimicking structural domains in the foot-and-mouth disease virus genome elicit a broad innate immune response in porcine cells triggered by RIG-I and TLR activationBorrego, BelénRodríguez Pulido, Miguel RamónRevilla Calvo, ConcepciónÁlvarez, BelénSobrino Castelló, FranciscoDomínguez, JavierSáiz, MargaritaViral sensorsAntiviralsNon-coding RNAInnate immunityFoot-and-mouthDisease virusThe innate immune system is the first line of defense against viral infections. Exploiting innate responses for antiviral, therapeutic and vaccine adjuvation strategies is being extensively explored. We have previously described, the ability of small in vitro RNA transcripts, mimicking the sequence and structure of different domains in the non-coding regions of the foot-and-mouth disease virus (FMDV) genome (ncRNAs), to trigger a potent and rapid innate immune response. These synthetic non-infectious molecules have proved to have a broad-range antiviral activity and to enhance the immunogenicity of an FMD inactivated vaccine in mice. Here, we have studied the involvement of pattern-recognition receptors (PRRs) in the ncRNA-induced innate response and analyzed the antiviral and cytokine profiles elicited in swine cultured cells, as well as peripheral blood mononuclear cells (PBMCs)This study was supported by grants AGL2011-24509 and AGL2014-58675 from the Spanish Ministerio de Economía y Competitividad (MINECO) and P2013/ABI-2906 from the Autonomous Community of Madrid. MRP was the holder of a “Juan de la Cierva” program fellowship from MINECO.Peer ReviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía, Industria y Competitividad (España)Comunidad de MadridConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720152017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/149836reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-58675-RP2013/ABI-2906/PLATESAhttps://doi.org/10.3390/v7072807Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1498362026-05-22T06:33:51Z
dc.title.none.fl_str_mv Synthetic RNAs mimicking structural domains in the foot-and-mouth disease virus genome elicit a broad innate immune response in porcine cells triggered by RIG-I and TLR activation
title Synthetic RNAs mimicking structural domains in the foot-and-mouth disease virus genome elicit a broad innate immune response in porcine cells triggered by RIG-I and TLR activation
spellingShingle Synthetic RNAs mimicking structural domains in the foot-and-mouth disease virus genome elicit a broad innate immune response in porcine cells triggered by RIG-I and TLR activation
Borrego, Belén
Viral sensors
Antivirals
Non-coding RNA
Innate immunity
Foot-and-mouth
Disease virus
title_short Synthetic RNAs mimicking structural domains in the foot-and-mouth disease virus genome elicit a broad innate immune response in porcine cells triggered by RIG-I and TLR activation
title_full Synthetic RNAs mimicking structural domains in the foot-and-mouth disease virus genome elicit a broad innate immune response in porcine cells triggered by RIG-I and TLR activation
title_fullStr Synthetic RNAs mimicking structural domains in the foot-and-mouth disease virus genome elicit a broad innate immune response in porcine cells triggered by RIG-I and TLR activation
title_full_unstemmed Synthetic RNAs mimicking structural domains in the foot-and-mouth disease virus genome elicit a broad innate immune response in porcine cells triggered by RIG-I and TLR activation
title_sort Synthetic RNAs mimicking structural domains in the foot-and-mouth disease virus genome elicit a broad innate immune response in porcine cells triggered by RIG-I and TLR activation
dc.creator.none.fl_str_mv Borrego, Belén
Rodríguez Pulido, Miguel Ramón
Revilla Calvo, Concepción
Álvarez, Belén
Sobrino Castelló, Francisco
Domínguez, Javier
Sáiz, Margarita
author Borrego, Belén
author_facet Borrego, Belén
Rodríguez Pulido, Miguel Ramón
Revilla Calvo, Concepción
Álvarez, Belén
Sobrino Castelló, Francisco
Domínguez, Javier
Sáiz, Margarita
author_role author
author2 Rodríguez Pulido, Miguel Ramón
Revilla Calvo, Concepción
Álvarez, Belén
Sobrino Castelló, Francisco
Domínguez, Javier
Sáiz, Margarita
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía, Industria y Competitividad (España)
Comunidad de Madrid
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Viral sensors
Antivirals
Non-coding RNA
Innate immunity
Foot-and-mouth
Disease virus
topic Viral sensors
Antivirals
Non-coding RNA
Innate immunity
Foot-and-mouth
Disease virus
description The innate immune system is the first line of defense against viral infections. Exploiting innate responses for antiviral, therapeutic and vaccine adjuvation strategies is being extensively explored. We have previously described, the ability of small in vitro RNA transcripts, mimicking the sequence and structure of different domains in the non-coding regions of the foot-and-mouth disease virus (FMDV) genome (ncRNAs), to trigger a potent and rapid innate immune response. These synthetic non-infectious molecules have proved to have a broad-range antiviral activity and to enhance the immunogenicity of an FMD inactivated vaccine in mice. Here, we have studied the involvement of pattern-recognition receptors (PRRs) in the ncRNA-induced innate response and analyzed the antiviral and cytokine profiles elicited in swine cultured cells, as well as peripheral blood mononuclear cells (PBMCs)
publishDate 2015
dc.date.none.fl_str_mv 2015
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/149836
url http://hdl.handle.net/10261/149836
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-58675-R
P2013/ABI-2906/PLATESA
https://doi.org/10.3390/v7072807
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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