Synergistic antipseudomonal effects of synthetic peptide AMP38 and carbapenems

The aim was to explore the antimicrobial activity of a synthetic peptide (AMP38) and its synergy with imipenem against imipenem-resistant Pseudomonas aeruginosa. The main mechanism of imipenem resistance is the loss or alteration of protein OprD. Time-kill and minimal biofilm eradication concentrati...

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Detalles Bibliográficos
Autores: Rudilla Mateo, Héctor, Fusté i Domínguez, Ester, Cajal Visa, Yolanda, Rabanal Anglada, Francesc, Vinuesa Aumedes, Teresa, Viñas, Miquel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/103103
Acceso en línea:https://hdl.handle.net/2445/103103
Access Level:acceso abierto
Palabra clave:Pseudomonas
Síntesi de pèptids
Pèptids
Biofilms
Antibiòtics
Peptide synthesis
Peptides
Antibiotics
Descripción
Sumario:The aim was to explore the antimicrobial activity of a synthetic peptide (AMP38) and its synergy with imipenem against imipenem-resistant Pseudomonas aeruginosa. The main mechanism of imipenem resistance is the loss or alteration of protein OprD. Time-kill and minimal biofilm eradication concentration (MBEC) determinations were carried out by using clinical imipenem-resistant strains. AMP38 was markedly synergistic with imipenem when determined in imipenem-resistant P. aeruginosa. MBEC obtained for the combination of AMP38 and imipenem was of 62.5 μg/mL, whereas the MBEC of each antimicrobial separately was 500 μg/mL. AMP38 should be regarded as a promising antimicrobial to fight MDR P. aeruginosa infections. Moreover, killing effect and antibiofilm activity of AMP38 plus imipenem was much higher than that of colistin plus imipenem.