Green space exposure and blood DNA methylation at birth and in childhood - A multi-cohort study

Green space exposure has been associated with improved mental, physical and general health. However, the underlying biological mechanisms remain largely unknown. The aim of this study was to investigate the association between green space exposure and cord and child blood DNA methylation. Data from...

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Autores: Aguilar Lacasaña, Sofía, Castro, Montserrat de, Dadvand, Payam, Escribà Montagut, Xavier, Fossati, Serena, González, Juan Ramón, Nieuwenhuijsen, Mark J., Vrijheid, Martine, Bustamante Pineda, Mariona
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/60713
Acceso en línea:http://hdl.handle.net/10230/60713
http://dx.doi.org/10.1016/j.envint.2024.108684
Access Level:acceso abierto
Palabra clave:Child blood
Cord blood
DMP
DMR
DNA methylation
Green space
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network_name_str España
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dc.title.none.fl_str_mv Green space exposure and blood DNA methylation at birth and in childhood - A multi-cohort study
title Green space exposure and blood DNA methylation at birth and in childhood - A multi-cohort study
spellingShingle Green space exposure and blood DNA methylation at birth and in childhood - A multi-cohort study
Aguilar Lacasaña, Sofía
Child blood
Cord blood
DMP
DMR
DNA methylation
Green space
title_short Green space exposure and blood DNA methylation at birth and in childhood - A multi-cohort study
title_full Green space exposure and blood DNA methylation at birth and in childhood - A multi-cohort study
title_fullStr Green space exposure and blood DNA methylation at birth and in childhood - A multi-cohort study
title_full_unstemmed Green space exposure and blood DNA methylation at birth and in childhood - A multi-cohort study
title_sort Green space exposure and blood DNA methylation at birth and in childhood - A multi-cohort study
dc.creator.none.fl_str_mv Aguilar Lacasaña, Sofía
Castro, Montserrat de
Dadvand, Payam
Escribà Montagut, Xavier
Fossati, Serena
González, Juan Ramón
Nieuwenhuijsen, Mark J.
Vrijheid, Martine
Bustamante Pineda, Mariona
author Aguilar Lacasaña, Sofía
author_facet Aguilar Lacasaña, Sofía
Castro, Montserrat de
Dadvand, Payam
Escribà Montagut, Xavier
Fossati, Serena
González, Juan Ramón
Nieuwenhuijsen, Mark J.
Vrijheid, Martine
Bustamante Pineda, Mariona
author_role author
author2 Castro, Montserrat de
Dadvand, Payam
Escribà Montagut, Xavier
Fossati, Serena
González, Juan Ramón
Nieuwenhuijsen, Mark J.
Vrijheid, Martine
Bustamante Pineda, Mariona
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Child blood
Cord blood
DMP
DMR
DNA methylation
Green space
topic Child blood
Cord blood
DMP
DMR
DNA methylation
Green space
description Green space exposure has been associated with improved mental, physical and general health. However, the underlying biological mechanisms remain largely unknown. The aim of this study was to investigate the association between green space exposure and cord and child blood DNA methylation. Data from eight European birth cohorts with a total of 2,988 newborns and 1,849 children were used. Two indicators of residential green space exposure were assessed: (i) surrounding greenness (satellite-based Normalized Difference Vegetation Index (NDVI) in buffers of 100 m and 300 m) and (ii) proximity to green space (having a green space ≥ 5,000 m2 within a distance of 300 m). For these indicators we assessed two exposure windows: (i) pregnancy, and (ii) the period from pregnancy to child blood DNA methylation assessment, named as cumulative exposure. DNA methylation was measured with the Illumina 450K or EPIC arrays. To identify differentially methylated positions (DMPs) we fitted robust linear regression models between pregnancy green space exposure and cord blood DNA methylation and between cumulative green space exposure and child blood DNA methylation. Two sensitivity analyses were conducted: (i) without adjusting for cellular composition, and (ii) adjusting for air pollution. Cohort results were combined through fixed-effect inverse variance weighted meta-analyses. Differentially methylated regions (DMRs) were identified from meta-analysed results using the Enmix-combp and DMRcate methods. There was no statistical evidence of pregnancy or cumulative exposures associating with any DMP (False Discovery Rate, FDR, p-value < 0.05). However, surrounding greenness exposure was inversely associated with four DMRs (three in cord blood and one in child blood) annotated to ADAMTS2, KCNQ1DN, SLC6A12 and SDK1 genes. Results did not change substantially in the sensitivity analyses. Overall, we found little evidence of the association between green space exposure and blood DNA methylation. Although we identified associations between surrounding greenness exposure with four DMRs, these findings require replication.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/60713
http://dx.doi.org/10.1016/j.envint.2024.108684
url http://hdl.handle.net/10230/60713
http://dx.doi.org/10.1016/j.envint.2024.108684
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Environ Int. 2024 Jun;188:108684
info:eu-repo/grantAgreement/EC/H2020/874583
info:eu-repo/grantAgreement/EC/H2020/733206
info:eu-repo/grantAgreement/EC/H2020/874739
info:eu-repo/grantAgreement/EC/FP7/308333
info:eu-repo/grantAgreement/ES/2PE/CEX2018-000806-S
info:eu-repo/grantAgreement/ES/3PE/PID2021-122855OB-I00
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
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spelling Green space exposure and blood DNA methylation at birth and in childhood - A multi-cohort studyAguilar Lacasaña, SofíaCastro, Montserrat deDadvand, PayamEscribà Montagut, XavierFossati, SerenaGonzález, Juan RamónNieuwenhuijsen, Mark J.Vrijheid, MartineBustamante Pineda, MarionaChild bloodCord bloodDMPDMRDNA methylationGreen spaceGreen space exposure has been associated with improved mental, physical and general health. However, the underlying biological mechanisms remain largely unknown. The aim of this study was to investigate the association between green space exposure and cord and child blood DNA methylation. Data from eight European birth cohorts with a total of 2,988 newborns and 1,849 children were used. Two indicators of residential green space exposure were assessed: (i) surrounding greenness (satellite-based Normalized Difference Vegetation Index (NDVI) in buffers of 100 m and 300 m) and (ii) proximity to green space (having a green space ≥ 5,000 m2 within a distance of 300 m). For these indicators we assessed two exposure windows: (i) pregnancy, and (ii) the period from pregnancy to child blood DNA methylation assessment, named as cumulative exposure. DNA methylation was measured with the Illumina 450K or EPIC arrays. To identify differentially methylated positions (DMPs) we fitted robust linear regression models between pregnancy green space exposure and cord blood DNA methylation and between cumulative green space exposure and child blood DNA methylation. Two sensitivity analyses were conducted: (i) without adjusting for cellular composition, and (ii) adjusting for air pollution. Cohort results were combined through fixed-effect inverse variance weighted meta-analyses. Differentially methylated regions (DMRs) were identified from meta-analysed results using the Enmix-combp and DMRcate methods. There was no statistical evidence of pregnancy or cumulative exposures associating with any DMP (False Discovery Rate, FDR, p-value < 0.05). However, surrounding greenness exposure was inversely associated with four DMRs (three in cord blood and one in child blood) annotated to ADAMTS2, KCNQ1DN, SLC6A12 and SDK1 genes. Results did not change substantially in the sensitivity analyses. Overall, we found little evidence of the association between green space exposure and blood DNA methylation. Although we identified associations between surrounding greenness exposure with four DMRs, these findings require replication.This work was supported by the European Union’s Horizon 2020 research and innovation program − ATHLETE project [grant number 874583], LIFECYCLE project [grant number 733206], LongITools project [grant number 874739] and HELIX project [grant number 308333]. Sofía Aguilar-Lacasaña was funded by the FI-AGAUR Predoctoral contract [2023 FI-2 00797]. ISGlobal acknowledges support from the Spanish Ministry of Science and Innovation and State Research Agency through the “Centro de Excelencia Severo Ochoa 2019–2023” Program [CEX2018-000806-S] and Fondo Europeo de Desarrollo Regional, UE [PID2021-122855OB-I00], and support from the Generalitat de Catalunya through the CERCA Program and Ministery of Research and Universities [2021 SGR 01563]. Details of funding for each study and for contributions of individual studies can be found in Appendix B: Supplementary methods. No funders listed here or in Appendix B influenced the study aim, design, analysis or interpretation of results. The results expressed here are those of the authors and not necessarily any listed funder.Elsevier202420242024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/60713http://dx.doi.org/10.1016/j.envint.2024.108684reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésEnviron Int. 2024 Jun;188:108684info:eu-repo/grantAgreement/EC/H2020/874583info:eu-repo/grantAgreement/EC/H2020/733206info:eu-repo/grantAgreement/EC/H2020/874739info:eu-repo/grantAgreement/EC/FP7/308333info:eu-repo/grantAgreement/ES/2PE/CEX2018-000806-Sinfo:eu-repo/grantAgreement/ES/3PE/PID2021-122855OB-I00© 2024 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).http://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/607132026-06-12T07:21:37Z
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